FOUR SOLVENT EXTRACTION OF CINNAMOMUM CAMPHORA XYLEM AND ANALYSIS OF THE ANTI-FUNGAL ACTIVITY OF THE EXTRACTIVES

Lili Quan, Ning Ji, Zhanguang Wang, Cuixiang Lu, Lin Hui
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Abstract

Four solvents including distilled water, acetone, ethyl acetate and petroleum ether were used to extract xylem of C. camphora. The differences in chemical compounds of xylem of C. camphorawere analyzed by gas chromatography/mass spectrometry(GC/MS)and the anti-fungal activityof C. camphoraextractives on Coriolus versicolor(CV), Trametes versicolor(TV),Poria vaporaria (PP) and Gloeophyllum trabeum(GT) were tested. The result showed that the chemical composition and relative content of the four different solvent extracts were different.A large number of chemical compounds in the C. camphoraextractives had a variety of biological activity and certain application value. The growth inhibitory rates of ethyl acetate extracts of C. camphora on PP, CV, TV and GTwere 52.24%, 52.51%, 43.26%, and 54.63%, respectively. According to the concentration for 50% of maximal effect, the inhibitory order on test fungus were GT > PP > CV > TV.
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樟木质部的四种溶剂提取及其抗真菌活性分析
采用蒸馏水、丙酮、乙酸乙酯和石油醚四种溶剂提取樟树木质部。采用气相色谱/质谱联用技术(GC/MS)分析了樟树木质部化学成分的差异,并测定了樟树提取物对花斑Coriolus versicolor(CV)、花斑Trametes versicolor(TV)、Poria vaporaria (PP)和Gloeophyllum trabeum(GT)的抑菌活性。结果表明,四种不同溶剂提取物的化学成分和相对含量不同。樟脑提取物中大量化合物具有多种生物活性,具有一定的应用价值。樟树乙酸乙酯提取物对PP、CV、TV和gt4种细菌的生长抑制率分别为52.24%、52.51%、43.26%和54.63%。以达到最大抑菌效果的50%浓度为顺序,对受试真菌的抑菌效果依次为GT > PP > CV > TV。
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