Time Dependent Changes in Progesterone Receptor Expression in Cumulus Cells During Meiotic Resumption of Porcine Oocytes

Journal of mammalian ova research Pub Date : 2003-10-01 DOI:10.1274/JMOR.20.113

M. Shimada, M. Nishibori

{"title":"Time Dependent Changes in Progesterone Receptor Expression in Cumulus Cells During Meiotic Resumption of Porcine Oocytes","authors":"M. Shimada, M. Nishibori","doi":"10.1274/JMOR.20.113","DOIUrl":null,"url":null,"abstract":"In this study, to investigate the time dependent changes in progesterone receptor (PR) expression in cumulus cells during meiotic resumption of porcine oocytes, each amount of PR-A and PR-B mRNA was analyzed by RT-PCR with primer sets for the PR-B region and the PR-A/B common region. The results showed that the levels of both PR-A/B and PR-B mRNA were very low in cumulus cells immediately recovered from their follicles. The cultivation with FSH and LH significantly increased the level of both PR-A/B and PR- B mRNA in cumulus cell of COCs, whereas the level of PR-B mRNA significantly decreased at 12-hr cultivation. Nevertheless, the higher level of PR-A/B mRNA was maintained up to 20-hr cultivation, suggesting that PR-A was mainly expressed in cumulus cells during cultivation from 12 hr to 20 hr. When COCs were cultured for 10 hr and then further cultured with RU486 for 10 hr, the proportion of oocytes undergoing GVBD significantly decreased in a dose dependent fashion. These results suggest that the high ratios of PR-A to PR-B in cumulus cells of COCs during 12-hr to 20-hr cultivation, are required for meiotic resumption of porcine cumulus-","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"30 1","pages":"113-117"},"PeriodicalIF":0.0000,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of mammalian ova research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1274/JMOR.20.113","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 2

Abstract

In this study, to investigate the time dependent changes in progesterone receptor (PR) expression in cumulus cells during meiotic resumption of porcine oocytes, each amount of PR-A and PR-B mRNA was analyzed by RT-PCR with primer sets for the PR-B region and the PR-A/B common region. The results showed that the levels of both PR-A/B and PR-B mRNA were very low in cumulus cells immediately recovered from their follicles. The cultivation with FSH and LH significantly increased the level of both PR-A/B and PR- B mRNA in cumulus cell of COCs, whereas the level of PR-B mRNA significantly decreased at 12-hr cultivation. Nevertheless, the higher level of PR-A/B mRNA was maintained up to 20-hr cultivation, suggesting that PR-A was mainly expressed in cumulus cells during cultivation from 12 hr to 20 hr. When COCs were cultured for 10 hr and then further cultured with RU486 for 10 hr, the proportion of oocytes undergoing GVBD significantly decreased in a dose dependent fashion. These results suggest that the high ratios of PR-A to PR-B in cumulus cells of COCs during 12-hr to 20-hr cultivation, are required for meiotic resumption of porcine cumulus-

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

猪卵母细胞减数分裂恢复过程中卵丘细胞孕酮受体表达的时间依赖性变化

为了研究猪卵母细胞减数分裂恢复过程中卵丘细胞中孕酮受体(PR)表达的时间依赖性变化，采用RT-PCR方法分析了PR-B区和PR- a /B共同区各引物的PR- a和PR-B mRNA表达量。结果表明，在刚从卵泡中恢复的积云细胞中，PR-A/B和PR-B mRNA的水平都很低。FSH和LH培养可显著提高COCs积云细胞PR- a /B和PR-B mRNA水平，培养12小时后PR-B mRNA水平显著降低。然而，PR-A/B mRNA在培养20小时后仍保持较高水平，表明PR-A主要在培养12 - 20小时的积云细胞中表达。COCs培养10小时后，再与RU486一起培养10小时，发生GVBD的卵母细胞比例呈剂量依赖性显著降低。这些结果表明，在培养12 ~ 20小时的COCs积云细胞中PR-A与PR-B的高比率是猪积云-恢复减数分裂所必需的

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Journal of mammalian ova research

自引率

0.00%

发文量