Expression of GPX1-mRNA and apoptosis related signal molecular in Keshan disease patients

Q3 Medicine 中华地方病学杂志 Pub Date : 2010-07-20 DOI:10.3760/CMA.J.ISSN.1000-4955.2010.04.002

Song Rui-xia, Xiong Yong-min, Zou Xiu-zhen, Duan Xiao-hong, Sun Wen-yan

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引用次数: 1

Abstract

Objective To investigate the meaning of expression of apoptosis related molecules NFKBp65 and p53 and GPX1-mRNA in patients with Keshan disease(KSD).Methods Sixteen chronic Keshan Disease patients were enrolled in KSD group according to electrocardiogram,chest X ray film and clinical examinations on 15,September in 2009,and 23 healthy people were included in control group from physical examination taken in The Second Affiliated Hospital of Xi'an Jiaotong University.Fresh blood(5 ml)was collected from antecubital vein of all subjects in the fasting state.Total mRNA and protein of blood sample were isolated using Trizol.GPX Assay Kit was used to detect GPX enzyme activity,and GPX1-mRNA expression was determined by SYBR Real-Time PCR.Meanwhile,expression of apoptosis related molecules NFKBp65 and p53 were determined by Western blot.Results GPX enzyme activity decreased significantly in KSD group[(108.61±14.10)U]compared with control group[(122.78±11.89)U,t=2.874,P<0.05],GPX1-mRNA level of KSD group(0.553±0.299)notably KSD group(0.802±0.057)compared with control group[(1.065±0.355),t=6.829,P<0.01].p53 increased in KSD group(1.604±0.191)compared with control group[(1.137±0.186),t=3.033,P<0.05].Conclusiom Decreased GPX1-mRNA expression may result in lower GPX enzyme activity of patients with KSD.Thus oxidative damage increases and cadioeyte apoptosis is activated by activating apoptosis signal pathway. Key words: Keshan disease; Apoptosis; Glutathion peroxidase; NFкBp65; p53

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克山病患者GPX1-mRNA及凋亡相关信号分子的表达

目的探讨细胞凋亡相关分子NFKBp65、p53及GPX1-mRNA在克山病(KSD)患者中的表达意义。方法根据2009年9月15日的心电图、胸片及临床检查将16例慢性克山病患者纳入KSD组，将在西安交通大学第二附属医院进行体检的健康人23例作为对照组。所有受试者在禁食状态下从肘前静脉采集新鲜血液(5ml)。用Trizol法分离总mRNA和总蛋白。采用GPX Assay Kit检测GPX酶活，SYBR Real-Time PCR检测GPX1-mRNA表达。Western blot检测细胞凋亡相关分子NFKBp65、p53的表达。结果KSD组GPX酶活性[(108.61±14.10)U]较对照组[(122.78±11.89)U,t=2.874,P<0.05]显著降低;KSD组GPX1-mRNA水平(0.553±0.299)显著降低(0.802±0.057)，较对照组[(1.065±0.355)，t=6.829,P<0.01]。KSD组p53水平(1.604±0.191)高于对照组(1.137±0.186)，t=3.033,P<0.05)。结论GPX1-mRNA表达降低可能导致KSD患者GPX酶活性降低。通过激活凋亡信号通路，增加氧化损伤，激活心肌细胞凋亡。关键词:克山病;细胞凋亡;谷胱甘肽过氧化物酶;NFкBp65;p53

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来源期刊

中华地方病学杂志我国对人类健康危害特别严重的地方性疾病：克山病、大骨节病、碘缺乏病、地方性氟中毒、地方性砷中毒、鼠疫、布鲁氏菌病、寄生虫、新冠肺炎等疾病，同时还报道多发性自然疫源性疾病。

CiteScore

1.60

自引率

0.00%

发文量

8714

期刊介绍： The Chinese Journal of Endemiology covers predominantly endemic diseases threatening health of the people in the areas affected by the diseases including Keshan disease, Kaschin-Beck Disease, iodine deficiency disorders, endemic fluorosis, endemic arsenism, plague, epidemic hemorrhagic fever, brucellosis, parasite diseases and the diseases related to local natural and socioeconomic conditions; and reports researches in the basic science, etiology, epidemiology, clinical practice, control as well as multidisciplinary studies on the diseases.