Transmembrane potential measurements on mammalian cells using the voltage sensitive dye annine-6

T. Berghofer, C. Eing, B. Flickinger, C. Gusbeth, R. Strassner, W. Frey, S. Schneider
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Abstract

Annine-6, a voltage sensitive dye, was used to evaluate the transmembrane potential sensitivity of different mammalian cell lines (KEK293, 22Rv1, and HeLa). Transmembrane potential was measured at different field strengths. To guarantee the comparability with theoretical charging models, the measurements have been performed well below the assumed critical value for incipient pore formation of approximately 1 kV/cm for a typical cell-diameter of 15 micrometer.A good agreement between theoretical predictions and data has been found considering the general shape of the measured curve, whereas discrepancies occurred considering the absolute amplitude.
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用电压敏感染料苯胺-6测量哺乳动物细胞的跨膜电位
采用电压敏感染料Annine-6评价不同哺乳动物细胞系(KEK293、22Rv1和HeLa)的跨膜电位敏感性。测定不同场强下的跨膜电位。为了保证与理论充电模型的可比性,在典型电池直径为15微米时,测量值远低于假设的初始孔隙形成临界值,约为1千伏/厘米。考虑到测量曲线的一般形状,理论预测和数据之间的一致性很好,而考虑到绝对振幅,则出现了差异。
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