Prevalence of blaTEM and blaVEB Genes in Multidrug-Resistant Acinetobacter baumannii Strains Isolated from Burn Wound Infection in Isfahan, Iran

Abstract

Article History Received: March 15 ,2020 Accepted: May 15 ,2020 Published: June 10 ,2020 [1] Hashim IA, Wdaah QH, Atya AA. Potential effect of ... [2] Peck MD. Epidemiology of burns throughout the world. Part I: Distribution and ... [3] Capoor MR., Sarabahi S, Tiwari VK, Narayanan RP. Fungal ... [4] Zubair M, Malik A, Ahmad J, Rizvi M, Farooqui KJ, Rizvi MW. A study of ... [5] Asadollahi P, Akbari M, Soroush S, Taherikalani M, Asadollahi K, Sayehmiri K, et al. Antimicrobial resistance patterns and their encoding genes ... [6] Rezaei A, Fazeli H, Moghadampour M, Halaji M, Faghri J. Determination of antibiotic resistance pattern and prevalence of OXA-type carbapenemases among Acinetobacter baumannii clinical isolates from inpatients in Isfahan, central ... [7] Rezaei A, Fazeli H, Halaji M, Moghadampour M, Faghri J. Prevalence of ... [8] Heidari H, Halaji M, Taji A, Kazemian H, Abadi MSS, Sisakht MT, et al. Molecular analysis of drug-resistant ... [9] García-Patiño MG, García-Contreras R, Licona-Limón P. The immune response against Acinetobacter baumannii, an ... [10] Kittinger C, Kirschner A, Lipp M, Baumert R, Mascher F, Farnleitner AH, et al. Antibiotic resistance of Acinetobacter spp. isolates from the river ... [11] JafariSales A, Bagherizadeh Y, Khalifehpour M, Abdoli-senejan M, Helali-Pargali R. Antibiotic resistance pattern and blaTEM gene expression in Acinetobacter baumannii isolated from clinical specimens ... [12] Ghaima KK, Abdulhassan AA, Mahdi ZH. Molecular study of ... [13] Torkar KG, Bedenić B. Antimicrobial susceptibility and characterization of metalloβ-lactamases, extended ... [14] Fazeli H, Taraghian A, Poursina F, Kamali R. Molecular ... [15] Sohail M, Rashid A, Aslam B, Waseem M, Shahid M, Akram M, et al. Antimicrobial ... [16] Rezai MS, Rafiei A, Ahangarkani F, Bagheri-Nesami M, Nikkhah A, Shafahi K, et al. Emergence ... [17] Burcu B, Acik L, Sultan L. Phenotypic and molecular ... [18] Vahidi Emami H, Khalilian M, Yadollahi Movahhed N. Antibiotic ... [19] Moosavian M, Sirous M, Shams N. Phenotypic and ... [20] Bali EB, Accedil L, Sultan N. Phenotypic ... [21] Salih TS, Shafeek RR. In silico detection ... [22] Abdar MH, Taheri-Kalani M, Taheri K, Emadi B, Hasanzadeh A, Sedighi A, et al. Prevalence of ... [23] Ahmadikiya F, Mosadegh A, Moradi M, Hossieni-Nave H. Antimicrobial ... [24] Azizi O, Shahcheraghi F. The frequency of blaPER, blaVEB, blaCTX-M, tetA and tetB genes among ... [25] Shahcheraghi F, Akbari Shahmirzadi N, Abbasalipour Bashash M, Jabbar H, Amirmozafari N. Detection ... [26] Safari M, Nejad ASM, Bahador A, Jafari R, Alikhani MY. Prevalence of ... Aims: Acinetobacter baumannii is an opportunistic pathogen that is resistant to many antibiotics including beta-lactams. Production of β-lactamases is the main mechanism of β-lactam resistance in A. baumannii strains. The aim of this study was to determine the frequency of blaTEM and blaVEB genes in clinical isolates of A. baumannii and the relationship between the antibiotic resistance and the presence of ESBL genes in strains isolated from burn wound infection in Isfahan. Materials & Methods: In this study, 123 MDR A. baumannii strains were isolated from burn wound infection. After antibiotic resistance evaluation using the Kirby-Bauer disc-diffusion method, all the isolates were evaluated with polymerase chain reaction (PCR) technique to detect ESBL genes, followed by statistical analysis by the end. Findings: Out of 123 A. baumannii isolates, 77 (62.60%) strains were ESBL positive according to the PCR results. The frequency of blaTEM and blaVEB genes was 52 (42.3%) and 67 (54.5%), respectively. There was a significant relationship between the antibiotic resistance and the presence of ESBL genes (blaTEM and blaVEB) in A. baumannii strains. Conclusion: The high prevalence of blaTEM and blaVEB genes in A. baumannii strains found in this study is the major concern about burn wound infections in Isfahan and Iran because of the complexity in treating infections caused by these strains. This study results highlighted the need for infection control measures to prevent the spread of resistant isolates and ESBL genes, especially in burn hospitals.