{"title":"Comparison of Major Bioactive Components of Various Solvent Extracts from Algerian Bunium incrassatum Umbellules and Antioxidant Activity","authors":"F. Toul, Amina Djendar","doi":"10.18596/jotcsa.1184342","DOIUrl":null,"url":null,"abstract":"The present study was designed to examine the chemical composition and the antioxidant activity of Bunium incrassatum extracts prepared in five different solvents with increasing polarities. RP-HPLC analysis resulted in the identification of 12 phenolic compounds, including 8 phenolic acids (caffeic acid, ellagic acid, ferulic acid, gallic acid, rosmarinic acid, sinapic acid, syringic acid, and vanillic acid) and 4 flavonoids (catechin, hesperetin, luteolin, and quercetin). Two different test systems were used to assess the antioxidant activity: DPPH and β-carotene/linoleic acid assays. Methanol extract exhibited the highest DPPH scavenging potential and even low inhibitory effect against β-carotene bleaching, whereas chloroform extract showed the highest effect against β-carotene bleaching and a weak scavenging potential. As expected, methanol extract recorded the highest content of total phenolics (26.57±0.15 mg GAE/g E), while chloroform extract has been found to be the richest in flavonoids.","PeriodicalId":17299,"journal":{"name":"Journal of the Turkish Chemical Society Section A: Chemistry","volume":"15 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Turkish Chemical Society Section A: Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18596/jotcsa.1184342","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The present study was designed to examine the chemical composition and the antioxidant activity of Bunium incrassatum extracts prepared in five different solvents with increasing polarities. RP-HPLC analysis resulted in the identification of 12 phenolic compounds, including 8 phenolic acids (caffeic acid, ellagic acid, ferulic acid, gallic acid, rosmarinic acid, sinapic acid, syringic acid, and vanillic acid) and 4 flavonoids (catechin, hesperetin, luteolin, and quercetin). Two different test systems were used to assess the antioxidant activity: DPPH and β-carotene/linoleic acid assays. Methanol extract exhibited the highest DPPH scavenging potential and even low inhibitory effect against β-carotene bleaching, whereas chloroform extract showed the highest effect against β-carotene bleaching and a weak scavenging potential. As expected, methanol extract recorded the highest content of total phenolics (26.57±0.15 mg GAE/g E), while chloroform extract has been found to be the richest in flavonoids.