Pellet Formulation of Trichoderma Isolated from Aceh with Potential for Biocontrol of Phytophthora Sp. on Cacao Sedlings

R. Sriwati, Tjut Chamzhurni, A. Alfizar, B. P. Soekarno, V. Maulidia, Irza Sahputra
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Abstract

Molleculler study was conducted to identify several species of Trichoderma isolate from several  plant (Pine, Cacao, Gliceria, Nutmeg, Bamboo, Coffee, Potato).  The growth of eight species Trichodermaafter pelleting formulation has been observed. Pellet Trichoderma harzianum have good ability to growth on PDA medium after 4 weeks storage. Base on their mycelium diameter growth on PDA, T. harzianum have selected as potential species on pellet formulation growth.  Several dose of pellet formulation have been applied for controlling Phythopthora disease. The application of T. harzianum pellets in the form of a 2 g / 100 ml (S1) suspension effective in inhibiting the development of Phytophthora sp in cacao seedlings, when the higher concentrations of T. harzianum pellets applied to cacao seeds,the disease severity increase. Pellet Trichoderma could be use as biological control agent of cacao seedling in certain dosage.
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亚齐木霉颗粒剂对可可幼苗疫霉防治潜力的研究
对从几种植物(松树、可可、甘油桃、肉豆蔻、竹子、咖啡、土豆)中分离得到的木霉进行了分子鉴定。观察了八种木霉在制粒后的生长情况。哈茨木霉颗粒在PDA培养基上保存4周后,具有良好的生长能力。根据其菌丝直径在PDA上的生长情况,选择了哈兹兰作为潜在的球团生长种。几种颗粒剂制剂已应用于防治疫霉病。以2 g / 100 ml (S1)悬浮液的形式施用哈兹兰微球,可有效抑制可可幼苗中疫霉的发育,当哈兹兰微球施用于可可种子时,病害严重程度增加。木霉颗粒剂在一定剂量下可作为可可幼苗的生物防治剂。
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