Defining Reference Ranges for Serum Anti-Müllerian Hormone on a Large Cohort of Normozoospermic Adult Men Highlights New Potential Physiological Functions of AMH on FSH Secretion and Sperm Motility.
Hamza Benderradji, Anne-Laure Barbotin, Maryse Leroy-Billiard, Julie Prasivoravong, François Marcelli, Christine Decanter, Geoffroy Robin, Valérie Mitchell, Jean-Marc Rigot, Antonino Bongiovanni, Florent Sauve, Luc Buée, Claude-Alain Maurage, Maryse Cartigny, Arnauld Villers, Vincent Prevot, Sophie Catteau-Jonard, Nicolas Sergeant, Paolo Giacobini, Pascal Pigny, Clara Leroy
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Abstract
Background: Few studies to date have attempted to measure serum anti-Müllerian hormone (AMH) levels in adult men, and solid references ranges have not yet been defined in a large cohort.
Objective: In this study, we aimed, first, to establish the reference ranges for serum AMH and AMH-to-total testosterone ratio (AMH/tT) in adult males. Second, we investigated the relationship between serum AMH and both reproductive hormones and semen parameters.
Methods: This single-center retrospective study included 578 normozoospermic adult men. Serum AMH concentrations were determined with an automated sandwich chemiluminescent immunoassay.
Results: The median serum AMH was 43.5 pmol/L. The 2.5th and 97.5th percentile values for serum AMH and AMH/tT were 16.4 and 90.3 pmol/L and 0.45 and 3.43, respectively. AMH was positively correlated with inhibin B and sperm concentration and negatively correlated with age, follicle-stimulating hormone (FSH), and progressive sperm motility. Interestingly, using immunofluorescence, we documented for the first time that AMH type II receptor (AMH-R2) is expressed in ejaculated human spermatozoa and gonadotrophic cells in the postmortem pituitary gland.
Conclusions: We establish a new age-specific reference range for serum AMH and AMH/tT. Moreover, AMH-R2 expression in human spermatozoa and gonadotrophic cells, together with the relationship between serum AMH levels and sperm motility or mean FSH levels, highlight new potential functions of AMH in regulating sperm motility or FSH secretion in adult men.
期刊介绍：
The upsurge of academic and political interest in regional and federal questions since the 1980s has been stimulated by the salience of regions in EU policy-making and the Structural Funds but also by regionalization and federalization processes in many Western states. The most striking example is the devolution occurring in the UK, but the process is at work all over Europe and in other parts of the world. These developments have led to many important research programmes and projects. Regional and Federal Studies is a refereed social science journal which provides an academic forum for the publication of international research on these issues. It is essential reading for both academics and practitioners in politics, administration and the business world.
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