Perbandingan Daya Hambat Ekstrak Metanol Daun dan Akar Tanaman Ona cina (Lantana camara) Terhadap Pertumbuhan Staphylococcus aureus dan Pseudomonas aeruginosa

Abstract

Ona cina ( Lantana camara) is a plant that has the ability as an anti-bacterial. The bioactive compounds of the ona cina plant as anti-bacterial are flavonols and lantanilic acid. This study aimed to compared the methanol extract of the leaves and roots of the ona cina plant in inhibiting the growth of Staphylococcus aureus and Pseudomonas aeruginosa . This research is a laboratory experimental study using the well diffusion method with 24 treatments. The treatment was repeated 2 times with various concentrations of 0.5%, 5%, 10%, 25%, and 50%, and erythromycin as a positive control and aquades as a negative control. The methanol extract of the leaves and roots of the ona cina plant was obtained from the maceration process with methanol solvent, then the growth inhibition test was carried out on the test bacteria. The parameters observed were the clear zone formed around the well.The diameter of the inhibition zone for the growth of the test bacteria at all concentrations of the leaf methanol extract of the ona cina plant was 0 mm while for the methanol extract of the ona cina plant root, the diameter of the inhibition zone on the growth of Staphylococcus aureus at a concentration of 2.5% was 17 mm and the highest is found at a concentration of 50% of 25 mm. While the lowest inhibition zone of root methanol extract on the growth of Pseudomonas aeruginosa was at a concentration of 2.5% at 22 mm and the highest was at a concentration of 50% at 30 mm. The methanol extract of the leaves of the ona cina plant did not have the ability to inhibit the growth of the test bacteria. However, the methanol extract of the ona cina root has the ability to inhibit the growth of Staphylococcus aureus and Pseudomonas aeruginosa .