Factors Influencing the Phagocytosis - Stimulated Glucose Oxidation in Porcine Leukocytes

Abstract

The stimulation of the D-(1-¹⁴C)glucose and D-(6-¹⁴C)glucose metabolism in pig leucocytes during the phagocytosis of bacteria and inert particles was studied. The following results were obtained:

1. The magnitude of phagocytosis-stimulated glucose oxidation is directly related to the nature and number of particles added.

2. Live bacteria stimulate the glucose metabolism to a greater extent than do a similar number of heat-killed organisms. As to the extent of the stimulation the species of bacteria offered for phagocytosis is crucial.

3. After in vitro addition of a lipopolysaccharide a stimulating effect is observed, a depression has been shown after the addition of hydrocortisone.