Pub Date : 1976-09-01DOI: 10.1016/S0300-872X(76)80070-4
Emmett J. Johnson , Gerald J. Domingue, Gloria D. Klein
Cell walls, membrane vesicles and the soluble cytoplasmic components have been isolated from Escherichia coli 0: 14 and Salmonella typhimurium and examined by electron microscopy as well as hemagglutination inhibition with heterologous antisera for enterobacterial common antigen (ECA). The outer and cytoplasmic membranes also were isolated and examined for common antigen. Although common antigen was present in the isolated surface structures it was not localized exclusively in these fractions. Additional experiments revealed that, whatever the nature of the integration of ECA of Kunin into the cell envelope, it is a much more stable association in E. coli 0: 14 than in S. typhimurium.
{"title":"Anatomical Locus of the Common Enterobacterial Antigen1","authors":"Emmett J. Johnson , Gerald J. Domingue, Gloria D. Klein","doi":"10.1016/S0300-872X(76)80070-4","DOIUrl":"10.1016/S0300-872X(76)80070-4","url":null,"abstract":"<div><p>Cell walls, membrane vesicles and the soluble cytoplasmic components have been isolated from Escherichia coli 0: 14 and Salmonella typhimurium and examined by electron microscopy as well as hemagglutination inhibition with heterologous antisera for enterobacterial common antigen (ECA). The outer and cytoplasmic membranes also were isolated and examined for common antigen. Although common antigen was present in the isolated surface structures it was not localized exclusively in these fractions. Additional experiments revealed that, whatever the nature of the integration of ECA of Kunin into the cell envelope, it is a much more stable association in E. coli 0: 14 than in S. typhimurium.</p></div>","PeriodicalId":101289,"journal":{"name":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","volume":"152 1","pages":"Pages 15-26"},"PeriodicalIF":0.0,"publicationDate":"1976-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80070-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78797396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1976-09-01DOI: 10.1016/S0300-872X(76)80068-6
H. Buschmann , B. Frohnwieser
The stimulation of the D-(1-14C)glucose and D-(6-14C)glucose metabolism in pig leucocytes during the phagocytosis of bacteria and inert particles was studied. The following results were obtained:
1. The magnitude of phagocytosis-stimulated glucose oxidation is directly related to the nature and number of particles added.
2. Live bacteria stimulate the glucose metabolism to a greater extent than do a similar number of heat-killed organisms. As to the extent of the stimulation the species of bacteria offered for phagocytosis is crucial.
3. After in vitro addition of a lipopolysaccharide a stimulating effect is observed, a depression has been shown after the addition of hydrocortisone.
{"title":"Factors Influencing the Phagocytosis - Stimulated Glucose Oxidation in Porcine Leukocytes","authors":"H. Buschmann , B. Frohnwieser","doi":"10.1016/S0300-872X(76)80068-6","DOIUrl":"10.1016/S0300-872X(76)80068-6","url":null,"abstract":"<div><p>The stimulation of the D-(1-<sup>14</sup>C)glucose and D-(6-<sup>14</sup>C)glucose metabolism in pig leucocytes during the phagocytosis of bacteria and inert particles was studied. The following results were obtained:</p><p>1. The magnitude of phagocytosis-stimulated glucose oxidation is directly related to the nature and number of particles added.</p><p>2. Live bacteria stimulate the glucose metabolism to a greater extent than do a similar number of heat-killed organisms. As to the extent of the stimulation the species of bacteria offered for phagocytosis is crucial.</p><p>3. After in vitro addition of a lipopolysaccharide a stimulating effect is observed, a depression has been shown after the addition of hydrocortisone.</p></div>","PeriodicalId":101289,"journal":{"name":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","volume":"152 1","pages":"Pages 1-9"},"PeriodicalIF":0.0,"publicationDate":"1976-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80068-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82118218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1976-09-01DOI: 10.1016/S0300-872X(76)80069-8
E. Paluska , Z. Hrkal, B. Turková
The rabbit antisera against human and canine hemoglobin and globin were produced and the immunological relatedness of human and canine hemoglobin and human and canine globin was proved.
制备了兔抗人、犬血红蛋白和珠蛋白的血清，证实了人、犬血红蛋白和人、犬珠蛋白的免疫学相关性。
{"title":"Antigenic Relationship between Human and Canine Hemoglobin and Globin","authors":"E. Paluska , Z. Hrkal, B. Turková","doi":"10.1016/S0300-872X(76)80069-8","DOIUrl":"10.1016/S0300-872X(76)80069-8","url":null,"abstract":"<div><p>The rabbit antisera against human and canine hemoglobin and globin were produced and the immunological relatedness of human and canine hemoglobin and human and canine globin was proved.</p></div>","PeriodicalId":101289,"journal":{"name":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","volume":"152 1","pages":"Pages 10-14"},"PeriodicalIF":0.0,"publicationDate":"1976-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80069-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83538452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1976-09-01DOI: 10.1016/S0300-872X(76)80071-6
P. Emmerling , H. Finger, M. Müller
The simultaneous injection of either 108 or 5 × 105 sheep erythrocytes (SE) and an allogeneic anti-SE serum into mice produced not only a suppression of the primary immune response, but, moreover, the secondary immune reaction elicited, either 4, 8, 12, 16 or 30 weeks after the primary antigenic stimulation, was found to be impaired. This was mainly demonstrated by the significantly reduced numbers of 7S antibody-synthesizing spleen cells. The suppression of the secondary immune responses is hardly compatible with the conception that the antibody-mediated immunosuppression is solely due to an inactivation of the antigenic determinants by the passively administered specific antibody in the periphery of the immune system. This objection against the so-called «peripheric theory» is supported by a further finding. When mice primarily immunized by a simultaneous injection of 108 SE and anti-SE-serum were treated with 2 × 107 SE 24 hours before boostering with 108 SE, in order to eliminate a possibly existing residual activity of the passively administered specific antibodies given together with the primary antigenic stimulus, the secondary 7S response was likewise found to be significantly suppressed. On the basis of these findings it is suggested that besides the «peripheric mechanism» a «central» effect plays a significant role in the phenomenon of antibodymediated immunosuppression, this being due to the reversible or irreversible inactivation of immunocompetent precursor cells by the attachment of antigen-antibody-complexes which results in an inhibition of their differentiation into antibody-producing cells.
{"title":"Experimentelle Untersuchungen über Wirkungsweise und Dauer der Antikörper-bedingten Immunsuppression1","authors":"P. Emmerling , H. Finger, M. Müller","doi":"10.1016/S0300-872X(76)80071-6","DOIUrl":"10.1016/S0300-872X(76)80071-6","url":null,"abstract":"<div><p>The simultaneous injection of either 10<sup>8</sup> or 5 × 10<sup>5</sup> sheep erythrocytes (SE) and an allogeneic anti-SE serum into mice produced not only a suppression of the primary immune response, but, moreover, the secondary immune reaction elicited, either 4, 8, 12, 16 or 30 weeks after the primary antigenic stimulation, was found to be impaired. This was mainly demonstrated by the significantly reduced numbers of 7S antibody-synthesizing spleen cells. The suppression of the secondary immune responses is hardly compatible with the conception that the antibody-mediated immunosuppression is solely due to an inactivation of the antigenic determinants by the passively administered specific antibody in the periphery of the immune system. This objection against the so-called «peripheric theory» is supported by a further finding. When mice primarily immunized by a simultaneous injection of 10<sup>8</sup> SE and anti-SE-serum were treated with 2 × 10<sup>7</sup> SE 24 hours before boostering with 10<sup>8</sup> SE, in order to eliminate a possibly existing residual activity of the passively administered specific antibodies given together with the primary antigenic stimulus, the secondary 7S response was likewise found to be significantly suppressed. On the basis of these findings it is suggested that besides the «peripheric mechanism» a «central» effect plays a significant role in the phenomenon of antibodymediated immunosuppression, this being due to the reversible or irreversible inactivation of immunocompetent precursor cells by the attachment of antigen-antibody-complexes which results in an inhibition of their differentiation into antibody-producing cells.</p></div>","PeriodicalId":101289,"journal":{"name":"Zeitschrift für Immunitaetsforschung, Experimentelle und Klinische Immunologie","volume":"152 1","pages":"Pages 27-41"},"PeriodicalIF":0.0,"publicationDate":"1976-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0300-872X(76)80071-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79356212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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