Immunomodulatory Potential of New Classical Herbomineral Formulation in Experimental Animals: Impact of Biofield Energy Healing Treatment

Abstract

A new proprietary herbomineral formulation was formulated, consisting of essential ingredients viz. herbal root extract ashwagandha and minerals (zinc, magnesium, and selenium). The aim of the study was to evaluate the immunomodulatory potential of Consciousness Energy Healing Treatment on the herbomineral formulation in male Sprague Dawley rats. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from seven renowned Biofield Energy Healers. Additionally, one group of animals also received Biofield Energy Treatment per se (day -15) by Biofield Energy Healers under similar conditions. The test formulation was evaluated for immunological parameters viz. humoral immune analysis, paw volume, hematological study, biochemistry, body weight, feed and water intake, and histopathology analysis were performed. The humoral immune response showed a significant increased primary and secondary antibody titre values by 26% and 150.4%, respectively in Biofield Energy Treatment per se group (G6, day -15), while primary and secondary antibody titre values were increased by 100.0% and 110.4%, respectively in the Biofield Energy Treated test formulation (G7, day -15) group compared with the disease control group (G2). The results of delayed type hypersensitivity showed a significant increase in the paw volume by 94.44% in the Biofield Energy Treated test formulation (G4) group with respect to the G2 group. However, a significant increase in paw volume by 61.11% and 44.44% was found in G6 and G7 group, respectively with respect to the G2 group. Hematological parameters showed a significant (p<0.001) increased platelet count by 85.6% in the G4 group, while 39.15% and 35.22% increase in the G6 and G7 groups, respectively compared with the G2 group. In biochemical analysis, a significant increase in calcium and phosphorus level were found in the G4 group compared with the G2 group. Animal weight parameters suggests that there were no treatment-related changes in any group, organ to body weight ratio, feed and water intake. The data described that the Biofield Energy Treated test formulation was found to be safe without any side effect during the course of the experiment. These data suggests that the Biofield Energy Treated test formulation and Biofield Energy Treatment per se can be used for autoimmune and inflammatory diseases, enhancing stress management and prevention, and anti-aging by improving overall health.