Antibiotic Resistance Bacteria Associated in Waters Discharges of a Hospital in Patna

Abstract

The isolation and screening of antibiotic resistance strains from three samples sites present in and around hospital of Patna resulted in isolation of 300 bacterial isolates. Out of which maximum number of isolates were obtained from site B while site A gave least number of isolates. 89 bacterial strains showed antibiotic resistance. Among these 89 isolates Site B had maximum number of antibiotic resistant isolates (51) followed by site C with 33 isolates. Among the four groups of antibiotic selected for investigation, the isolates showed highest antibiotic resistance against fluoroquinolones with 1, 20 and 14 isolates from site A, site B and site C respectively showing antibiotic resistance. Site B isolates showed significant resistance against group III (aminoglycosides) antibiotic. Site C isolates also showed maximum resistance against Group IV antibiotics followed by Group II, III and Group I. Site A sample showed resistance against Ciprofloxacin, however it showed sensitivity against all other antibiotics. Study showed that there was a dynamic flux in the response of E. coli strains against antibiotic. Antibiotic resistant E. coli was present in wastewater. Key words: bacterial isolates, antibiotic resistance, E. coli.