Antioxidant and inhibitory activity of Roselle Extract (Hibiscus sabdariffa L.) against Methicillin-Resistant Staphylococcus aureus (MRSA)

Berkala Ilmiah Kimia Farmasi Pub Date : 2021-11-08 DOI:10.20473/bikfar.v8i1.31209

N. Fikriyah, I. Isnaeni, Asri Darmawati

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Abstract

Background: Hibiscus sabdariffa known as roselle, which belongs to the family Malvaceae, grows in sub-tropical and tropical region including Indonesia. Several studies have reported the benefits of roselle for health. Roselle extract has been shown to have antibacterial effect as a support for antibacterial therapy, especially for case of antibiotic resistance, and antioxidant effect which can neutralize free radicals. Purpose: The aim of this study was obtaining the minimum inhibitory concentration (MIC) and inhibition concentration (IC50) of roselle extract. Methods: Antibacterial effect of roselle extract against Methicillin-Resistant Staphylococcus aureus (MRSA) was tested in nutrient agar media using diffusion method. Antioxidant activity of roselle extract was performed by DPPH. The violet color of DPPH solutions that was reduced by roselle extract were measured using visible spectrophotometer at the wavelength of 516 nm. Result: The result of this study obtained the (MIC) and IC50 of roselle extract were 2,5% and 1251±202,32 ppm, respectively. Conclusion: It can be concluded that roselle extract have antibacterial effect against MRSA and antioxidant effect.

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芙蓉提取物对耐甲氧西林金黄色葡萄球菌(MRSA)的抗氧化和抑制作用

背景:木槿(Hibiscus sabdariffa)，俗称玫瑰，属于锦葵科，生长在包括印度尼西亚在内的亚热带和热带地区。一些研究报告了玫瑰茄对健康的益处。玫瑰提取物已被证明具有抗菌作用，作为抗菌治疗的支持，特别是在抗生素耐药的情况下，具有抗氧化作用，可以中和自由基。目的:研究玫瑰茄提取物的最低抑菌浓度(MIC)和抑菌浓度(IC50)。方法:采用扩散法在营养琼脂培养基上检测玫瑰叶提取物对耐甲氧西林金黄色葡萄球菌(MRSA)的抑菌作用。采用DPPH法测定玫瑰茄提取物的抗氧化活性。用可见分光光度计在516 nm波长处测定玫瑰茄提取物还原DPPH溶液的紫色。结果:本研究得到的玫瑰提取物(MIC)为2.5%，IC50为1251±202,32 ppm。结论:玫瑰茄提取物对MRSA具有抗菌作用和抗氧化作用。

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