Biosynthesis and Characteristics of Polyhydroxyalkanoates. 1. Polyhydroxybutyrates of Azotobacter vinelandii N-15

IF 1 Q4 CHEMISTRY, MULTIDISCIPLINARY Chemistry & Chemical Technology Pub Date : 2020-12-15 DOI:10.23939/chcht14.04.463
I. Semeniuk, T. Pokynbroda, V. Kochubei, H. Midyana, O. Karpenko, V. Skorokhoda
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引用次数: 4

Abstract

The biosynthesis of cellular polymers of Azotobacter vinelandii N-15 strain using molasses as a carbon source has been optimized. The highest yield of polymer (25.8 % of cell mass) was obtained on a nutrient medium with a molasses concentration of 50 g/l. Using TL-chromatography and IR-spectroscopy the obtained product was identified as polyhydroxybutyrate (PHB), and its properties were investigated. The wetting contact angle was used to characterize the biopolymer film surface properties. According to the results of thermal and thermomechanical studies, it was found that the obtained РHB is characterized by a high thermal stability and heat resistance: the melting point is 462 K; deep destruction and thermooxidative processes begin at the temperatures above 567 K.
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聚羟基烷酸酯的生物合成及特性研究。固氮菌vinelandii N-15的聚羟基丁酸盐
优化了以糖蜜为碳源的固氮菌N-15细胞聚合物的生物合成工艺。在糖蜜浓度为50 g/l的营养培养基上,聚合物的产率最高(细胞质量的25.8%)。利用tl -色谱和ir -光谱对所得产物进行了鉴定,并对其性质进行了研究。用润湿接触角表征了生物聚合物膜的表面特性。根据热学和热力学研究结果,发现所得РHB具有较高的热稳定性和耐热性:熔点为462 K;深度破坏和热氧化过程开始于567 K以上的温度。
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来源期刊
Chemistry & Chemical Technology
Chemistry & Chemical Technology CHEMISTRY, MULTIDISCIPLINARY-
CiteScore
1.70
自引率
44.40%
发文量
60
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