Antimicrobial Resistance Pattern of Clinical Isolates of Pseudomonas aeruginosa from Urinary Tract Infections in Wukari, Taraba state, Nigeria

M. Daji, T. Ade, Hyelnaya Shamdama Cletus, Aishatu Muhammad Bello, Panshak Joseph
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Abstract

Pseudomonas aeruginosa is a potent nosocomial pathogen of immunocompromised individuals, causing several infections while also resisting chemotherapy with conventional antimicrobial agents. Hence, this study was carried out to determine the antimicrobial resistance pattern of P. aeruginosa associated with urinary tract infections (UTIs) in Wukari, Taraba State. Thirty (30) voided midstream urine were collected from clinically diagnosed UTI patients attending Wukari general hospital and cultured aerobically on MacConkey agar and cysteine-lactose-electrolyte-deficient (CLED) agar. Bacterial isolates were identified by Gram staining and conventional biochemical tests. Antimicrobial sensitivity testing was done using the modified Kirby-Bauer method of the disc diffusion test. A total of 46 uropathogens were isolated of which 8 (17.39%) were identified as P. aeruginosa. Of these 8 isolates, 6 (75%) were isolated from male patients while 2 (25%) were isolated from female patients. All isolates of P. aeruginosa were susceptible to imipenem, ofloxacin, gentamicin, and levofloxacin. The resistances included resistance to amoxicillin-clavulanate (100%), cefepime (87.5%), cefotaxime (87.5%), ampiclox (75%), ceftriaxone (62.5%), cefuroxime (62.5%), and nalidixic acid (37.5%). High resistance rates against penicillins and cephalosporins are an indication of intrinsic resistance in P. aeruginosa. Hence, chemotherapy with imipenem, ofloxacin, gentamicin, and levofloxacin should be regularly monitored to prevent the development of resistant strains.
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尼日利亚塔拉巴州乌卡里市尿路感染铜绿假单胞菌临床分离株的耐药模式
铜绿假单胞菌是免疫功能低下个体的一种强有力的医院病原体,引起几种感染,同时也抵抗常规抗菌药物的化疗。因此,本研究旨在确定塔拉巴州乌卡里与尿路感染(uti)相关的铜绿假单胞菌的抗微生物药物耐药性模式。从乌卡里总医院临床诊断为UTI的患者收集30例中游尿,并在MacConkey琼脂和半胱氨酸-乳糖-电解质缺乏(半胱氨酸-乳糖-电解质缺乏)琼脂上进行有氧培养。采用革兰氏染色法和常规生化试验对分离菌进行鉴定。药敏试验采用改良的Kirby-Bauer纸片扩散试验法。共分离尿路病原菌46株,其中铜绿假单胞菌8株(17.39%)。8株分离株中,男性分离株6株(75%),女性分离株2株(25%)。铜绿假单胞菌对亚胺培南、氧氟沙星、庆大霉素和左氧氟沙星均敏感。耐药率为:阿莫西林-克拉维酸(100%)、头孢吡肟(87.5%)、头孢噻肟(87.5%)、氨苄氯(75%)、头孢曲松(62.5%)、头孢呋辛(62.5%)、萘啶酸(37.5%)。对青霉素和头孢菌素的高耐药率是铜绿假单胞菌内在耐药的指示。因此,化疗时应定期监测亚胺培南、氧氟沙星、庆大霉素和左氧氟沙星,以防止耐药菌株的产生。
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