{"title":"Occurrence, Identification and Preliminary Biological Control of Bulb Rot of Onion (Allium cepa)","authors":"K. Srimai, A. Akarapisan","doi":"10.12982/cmjs.2023.031","DOIUrl":null,"url":null,"abstract":"Onion bulb rot was observed in low-temperature warehouses in Mae Wang District, Chiang Mai, Thailand in 2022. The pathogens were isolated and identifi ed as Pantoea agglomerans and Burkholderia gladioli based on morphological and biochemical characterization, pathogenicity tests, and 16S rRNA gene sequences. Biological control is regarded as a safer and more sustainable strategy than chemical control and has elicited great attention for disease control. Bacillus sp. isolate BB22 was isolated from rhizosphere soil and was evaluated for its preliminary potential to inhibit the growth of onion bulb rot bacterial pathogens by the dual culture technique. The results showed inhibition zones of P. agglomerans of 30.1 mm and B. gladioli of 13.0 mm. Molecular identifi cation of Bacillus sp. isolate BB22 was performed by using 16S rRNA and gyrA gene sequences and confi rmed as Bacillus velezensis. PCR amplifi cation of the bmyB, fenD, ituA, and srfAA genes coding for bacillomycin, fengycin D, iturin A, and surfactin, respectively, showed the potential for the production of these antibiotics. This study revealed the potential application of B. velezensis as a biocontrol agent against bulb rot-causing bacterial pathogens of onions.","PeriodicalId":9884,"journal":{"name":"Chiang Mai Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.6000,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chiang Mai Journal of Science","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.12982/cmjs.2023.031","RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Onion bulb rot was observed in low-temperature warehouses in Mae Wang District, Chiang Mai, Thailand in 2022. The pathogens were isolated and identifi ed as Pantoea agglomerans and Burkholderia gladioli based on morphological and biochemical characterization, pathogenicity tests, and 16S rRNA gene sequences. Biological control is regarded as a safer and more sustainable strategy than chemical control and has elicited great attention for disease control. Bacillus sp. isolate BB22 was isolated from rhizosphere soil and was evaluated for its preliminary potential to inhibit the growth of onion bulb rot bacterial pathogens by the dual culture technique. The results showed inhibition zones of P. agglomerans of 30.1 mm and B. gladioli of 13.0 mm. Molecular identifi cation of Bacillus sp. isolate BB22 was performed by using 16S rRNA and gyrA gene sequences and confi rmed as Bacillus velezensis. PCR amplifi cation of the bmyB, fenD, ituA, and srfAA genes coding for bacillomycin, fengycin D, iturin A, and surfactin, respectively, showed the potential for the production of these antibiotics. This study revealed the potential application of B. velezensis as a biocontrol agent against bulb rot-causing bacterial pathogens of onions.
期刊介绍:
The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so.