{"title":"Yeast as a Versatile System for the Expression of Heterologous Proteins","authors":"R. Soni","doi":"10.9734/bpi/imb/v1/12303d","DOIUrl":null,"url":null,"abstract":"The yeast, Saccharomyces cerevisiae has been extensively used for heterologous protein expression due to its ease of genetic manipulation, faster generation time, availability of integrative and episomal vectors and promoters that can be easily turned on and off using simple chemical moieties. In addition, being a simplest eukaryote, it offers several advantages in the expression of mammalian proteins regarding post-translational modifications and expression of proteins that are membrane bound. This article describes development of a strain for the expression of membrane bound human drug metabolism enzymes.","PeriodicalId":13595,"journal":{"name":"Innovations in Microbiology and Biotechnology Vol. 1","volume":"4 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Innovations in Microbiology and Biotechnology Vol. 1","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.9734/bpi/imb/v1/12303d","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The yeast, Saccharomyces cerevisiae has been extensively used for heterologous protein expression due to its ease of genetic manipulation, faster generation time, availability of integrative and episomal vectors and promoters that can be easily turned on and off using simple chemical moieties. In addition, being a simplest eukaryote, it offers several advantages in the expression of mammalian proteins regarding post-translational modifications and expression of proteins that are membrane bound. This article describes development of a strain for the expression of membrane bound human drug metabolism enzymes.
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