Pub Date : 2021-08-30DOI: 10.9734/bpi/imb/v1/3872f
S. Ibekwe, G. Okpokwasili
{"title":"Bacterial Treatment of Drill Cuttings: Experimental Investigation","authors":"S. Ibekwe, G. Okpokwasili","doi":"10.9734/bpi/imb/v1/3872f","DOIUrl":"https://doi.org/10.9734/bpi/imb/v1/3872f","url":null,"abstract":"","PeriodicalId":13595,"journal":{"name":"Innovations in Microbiology and Biotechnology Vol. 1","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88528873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-30DOI: 10.9734/bpi/imb/v1/10253d
Y. Danso
A survey study was conducted in six major okra growing Districts of Ghana between August and December 2014. It was to assess the prevalence, incidence, and severity of root-knot disease in okra incited by root-knot nematodes. Fifty-four sole okra fields were purposively selected from 18 localities for the study. On an acre okra farm, 25 representative plants were sampled in an N-shaped pattern and assessed for incidence and degree of knotting following a rating scale of 0 to 10. Seventy-six percent of the okra fields assessed were found to be positive for root-knot nematodes infection. Seventy-eight, 67, 67, 89, 100, and 56% root-knot nematodes infection prevalence were recorded in the Kintampo North, Atebubu Amantin, Wenchi, Ejura Sekyedumase, Offinso North, and Atwima Nwabiagya Districts, respectively. The highest infection incidence (95%) was recorded at Sentiatia in the Offinso North District while the lowest (22%), at Amponsakrom in the Wenchi District. Galling indices ranged between 5 and 8 across the Districts. The root-knot nematodes infestation menace in okra was common across the study area. Sustainable management strategies must be designed by crop protectionists and disseminated among okra farmers against the pest to boost okra production in Ghana and beyond.
{"title":"Root-knot Nematode Infections in Okra in the Middle Belt of Ghana","authors":"Y. Danso","doi":"10.9734/bpi/imb/v1/10253d","DOIUrl":"https://doi.org/10.9734/bpi/imb/v1/10253d","url":null,"abstract":"A survey study was conducted in six major okra growing Districts of Ghana between August and December 2014. It was to assess the prevalence, incidence, and severity of root-knot disease in okra incited by root-knot nematodes. Fifty-four sole okra fields were purposively selected from 18 localities for the study. On an acre okra farm, 25 representative plants were sampled in an N-shaped pattern and assessed for incidence and degree of knotting following a rating scale of 0 to 10. Seventy-six percent of the okra fields assessed were found to be positive for root-knot nematodes infection. Seventy-eight, 67, 67, 89, 100, and 56% root-knot nematodes infection prevalence were recorded in the Kintampo North, Atebubu Amantin, Wenchi, Ejura Sekyedumase, Offinso North, and Atwima Nwabiagya Districts, respectively. The highest infection incidence (95%) was recorded at Sentiatia in the Offinso North District while the lowest (22%), at Amponsakrom in the Wenchi District. Galling indices ranged between 5 and 8 across the Districts. The root-knot nematodes infestation menace in okra was common across the study area. Sustainable management strategies must be designed by crop protectionists and disseminated among okra farmers against the pest to boost okra production in Ghana and beyond.","PeriodicalId":13595,"journal":{"name":"Innovations in Microbiology and Biotechnology Vol. 1","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80749783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-30DOI: 10.9734/bpi/imb/v1/11427d
Le Quang Khoi, T. T. Ngon, C. N. Diệp
Poly-phosphate accumulating bacteria (PAB) is an important bacterial group that can take up large amounts of phosphate and accumulate as intracellular poly-phosphate, contributing to biological phosphorus removal in waste-water treatment. The study was conducted to analyze a genetic diversity of PAB community isolated from samples of water and sludge of intensive catfish ponds and effluent water and sludge obtained from piggery waste-water treated by bio-digesters in the Mekong Delta, Vietnam. In a total of 439 isolates, there are 48 isolates that have the potential of accumulating intracellular poly-phosphate shown main characters included shaped like a rods or short rod-shaped; motile, twitching movements or non-motile; expression of poly-phosphate electron dense granules within the cells; accumulated poly-phosphate from poly-phosphate kinase gene 1; the content of intracellular poly-phosphate varied from 10-9 to 10-12 mg/cell. Based on phylogenetic tree for partial 16S rRNA genes, there are 22 strains isolated from intensive catfish ponds included in four classes: Bacilli, Actinobacteria, Beta-proteobacteria, Gamma-proteobacteria, and 26 strains isolated from piggery waste-water treated by bio-digesters included in four classes: Bacilli, Actinobacteria, Alpha-proteobacteria, Gamma-proteobacteria. The process of analysis and comparison of genetic diversity of 48 isolates showed 16S rRNA sequences have nucleotide regions of high variability interspersed with nucleotide regions of low variability. The variation of o and Pi index formed the different types of haplotype in population of PAB, there were 25 haplotypes (genotypes) from 48 sequences. The difference of the structure of haplotypes formed a high diversity between them (Hd=0.91). The levels of haplotype diversity created many genotypes for the genetic variation and the ability to adapt to the environment in the evolutionary process of bacterial strains capable ofhigh accumulating poly-P. Comparison of genetic diversity between populations of bacteria isolated from two sampling places, the results showed that the nucleotide and haplotype diversity of the strains isolated in piggery waste-water treated biodigesters (Pi=0.16, h=14) were lower and the genetic conservation was higher than isolated strains in intensive catfish ponds (Pi=0.18, h=16). This is scientific basis for the selection of the sample sources for the isolation and selection of poly-phosphate accumulating strains.
{"title":"Study on Genetic Diversity of Poly-phosphate Accumulating Bacteria Isolated from Both Wastewater of Piggery and Catfish Ponds in the Mekong Delta","authors":"Le Quang Khoi, T. T. Ngon, C. N. Diệp","doi":"10.9734/bpi/imb/v1/11427d","DOIUrl":"https://doi.org/10.9734/bpi/imb/v1/11427d","url":null,"abstract":"Poly-phosphate accumulating bacteria (PAB) is an important bacterial group that can take up large amounts of phosphate and accumulate as intracellular poly-phosphate, contributing to biological phosphorus removal in waste-water treatment. The study was conducted to analyze a genetic diversity of PAB community isolated from samples of water and sludge of intensive catfish ponds and effluent water and sludge obtained from piggery waste-water treated by bio-digesters in the Mekong Delta, Vietnam. In a total of 439 isolates, there are 48 isolates that have the potential of accumulating intracellular poly-phosphate shown main characters included shaped like a rods or short rod-shaped; motile, twitching movements or non-motile; expression of poly-phosphate electron dense granules within the cells; accumulated poly-phosphate from poly-phosphate kinase gene 1; the content of intracellular poly-phosphate varied from 10-9 to 10-12 mg/cell. Based on phylogenetic tree for partial 16S rRNA genes, there are 22 strains isolated from intensive catfish ponds included in four classes: Bacilli, Actinobacteria, Beta-proteobacteria, Gamma-proteobacteria, and 26 strains isolated from piggery waste-water treated by bio-digesters included in four classes: Bacilli, Actinobacteria, Alpha-proteobacteria, Gamma-proteobacteria. The process of analysis and comparison of genetic diversity of 48 isolates showed 16S rRNA sequences have nucleotide regions of high variability interspersed with nucleotide regions of low variability. The variation of o and Pi index formed the different types of haplotype in population of PAB, there were 25 haplotypes (genotypes) from 48 sequences. The difference of the structure of haplotypes formed a high diversity between them (Hd=0.91). The levels of haplotype diversity created many genotypes for the genetic variation and the ability to adapt to the environment in the evolutionary process of bacterial strains capable ofhigh accumulating poly-P. Comparison of genetic diversity between populations of bacteria isolated from two sampling places, the results showed that the nucleotide and haplotype diversity of the strains isolated in piggery waste-water treated biodigesters (Pi=0.16, h=14) were lower and the genetic conservation was higher than isolated strains in intensive catfish ponds (Pi=0.18, h=16). This is scientific basis for the selection of the sample sources for the isolation and selection of poly-phosphate accumulating strains.","PeriodicalId":13595,"journal":{"name":"Innovations in Microbiology and Biotechnology Vol. 1","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83131832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-30DOI: 10.9734/bpi/imb/v1/4005f
T. B. Naik, V. Mane, A. Upadhya
Background: Enterococci were previously thought to be less virulent in healthy people, but they have recently emerged as important opportunistic pathogens, particularly in hospitalized patients, due to their ability to colonize the gastrointestinal tract for long periods, which is a key factor in the development of drug resistance and has become a major treatment roadblock. Objectives: To determine the species of Enterococci isolated from different clinical samples. 2. To determine the antimicrobial susceptibility pattern of Enterococci species. Methods and Materials: A cross-sectional study was carried out at a private tertiary care hospital in the Shivamogga district of Karnataka, using secondary data of Enterococcal species isolated from various clinical samples such as urine, blood, pus, sputum, and sterile body fluids that were maintained in the Microbiology laboratory registers for a period of one year from January 2014 to December 2014. Results: Out of 66 enterococcal isolates from various clinical samples majority i.e. 32 (48.48%) were found in urine, followed by pus 22 (33.33%). The majority of Enterococcus was seen in females 34 (51.51%). Enterococcus faecalis was the most common isolate, accounting for 56 (84.84%) of the total. Sensitivity to Linezolid and Vancomycin was found in all of the isolates. Maximum resistance was seen against Penicillin i.e. 26 (76.47%). Conclusion: Given the general trend of rising drug resistance and the occurrence of a wide range of Enterococcus species, ongoing surveillance of antimicrobial resistance in enterococci is required to propose suitable therapy.
{"title":"An Analysis of Data of Enterococci Species and Its Antibiogram at a Tertiary Care Hospital in Karnataka","authors":"T. B. Naik, V. Mane, A. Upadhya","doi":"10.9734/bpi/imb/v1/4005f","DOIUrl":"https://doi.org/10.9734/bpi/imb/v1/4005f","url":null,"abstract":"Background: Enterococci were previously thought to be less virulent in healthy people, but they have recently emerged as important opportunistic pathogens, particularly in hospitalized patients, due to their ability to colonize the gastrointestinal tract for long periods, which is a key factor in the development of drug resistance and has become a major treatment roadblock. \u0000Objectives: To determine the species of Enterococci isolated from different clinical samples. 2. To determine the antimicrobial susceptibility pattern of Enterococci species. \u0000Methods and Materials: A cross-sectional study was carried out at a private tertiary care hospital in the Shivamogga district of Karnataka, using secondary data of Enterococcal species isolated from various clinical samples such as urine, blood, pus, sputum, and sterile body fluids that were maintained in the Microbiology laboratory registers for a period of one year from January 2014 to December 2014. \u0000Results: Out of 66 enterococcal isolates from various clinical samples majority i.e. 32 (48.48%) were found in urine, followed by pus 22 (33.33%). The majority of Enterococcus was seen in females 34 (51.51%). Enterococcus faecalis was the most common isolate, accounting for 56 (84.84%) of the total. Sensitivity to Linezolid and Vancomycin was found in all of the isolates. Maximum resistance was seen against Penicillin i.e. 26 (76.47%). \u0000Conclusion: Given the general trend of rising drug resistance and the occurrence of a wide range of Enterococcus species, ongoing surveillance of antimicrobial resistance in enterococci is required to propose suitable therapy.","PeriodicalId":13595,"journal":{"name":"Innovations in Microbiology and Biotechnology Vol. 1","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89466597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-30DOI: 10.9734/bpi/imb/v1/2243e
W. Coulibaly, F. Camara, K. Bouatenin, A. Kouamé, Y. Bi
The present study was aimed to select strains of Saccharomyces cerevisiae and Candida tropicalis based on their ability to tolerate ethanol toxicity and to evaluate the expression level of he hydrophillins encoding genes TIF 11 and YJL144W to express encoding for hydrophilins. Freeze-drying is a popular dehydration method for preserving microorganisms. . In order to produce freeze-dried yeast starter culture for the brewing purpose of African traditional sorghum beer, we tested tolerance to ethanol stress and evaluated the relative expression level of genes TIF 11 and YJL144W encoding the hydrophilins. Among the strains tested, the best viability rate to ethanol stress (7.5% ethanol (v/v)) was found with Saccharomyces cerevisiae F12–7 and Candida tropicalis C0–7 respectively with 95% and 80%. For Saccharomyces cerevisiae strains, the strain F12-7, which had distinguished itself from other strains in previous tests, the TIF11 and YJL144W genes were the least expressed. For C. tropicalis strains, the statistical analyzes of the relative expression levels from the Tukey test revealed no difference between the strains for the 2 genes (P> 0.05). It is concluded that before freeze-drying starter production other investigations are needed in order to evaluate yeast performance. Also, the pathogenicity of Candida tropicalis strains will be studied for the using potentially non-pathogen strains.
{"title":"Determining the Potentialities of Yeast Strains to be Used as Freeze- Drying Starters for the Production of Traditional Sorghum Beer in Côte d’Ivoire","authors":"W. Coulibaly, F. Camara, K. Bouatenin, A. Kouamé, Y. Bi","doi":"10.9734/bpi/imb/v1/2243e","DOIUrl":"https://doi.org/10.9734/bpi/imb/v1/2243e","url":null,"abstract":"The present study was aimed to select strains of Saccharomyces cerevisiae and Candida tropicalis based on their ability to tolerate ethanol toxicity and to evaluate the expression level of he hydrophillins encoding genes TIF 11 and YJL144W to express encoding for hydrophilins. \u0000Freeze-drying is a popular dehydration method for preserving microorganisms. . In order to produce freeze-dried yeast starter culture for the brewing purpose of African traditional sorghum beer, we tested tolerance to ethanol stress and evaluated the relative expression level of genes TIF 11 and YJL144W encoding the hydrophilins. Among the strains tested, the best viability rate to ethanol stress (7.5% ethanol (v/v)) was found with Saccharomyces cerevisiae F12–7 and Candida tropicalis C0–7 respectively with 95% and 80%. For Saccharomyces cerevisiae strains, the strain F12-7, which had distinguished itself from other strains in previous tests, the TIF11 and YJL144W genes were the least expressed. For C. tropicalis strains, the statistical analyzes of the relative expression levels from the Tukey test revealed no difference between the strains for the 2 genes (P> 0.05). It is concluded that before freeze-drying starter production other investigations are needed in order to evaluate yeast performance. Also, the pathogenicity of Candida tropicalis strains will be studied for the using potentially non-pathogen strains.","PeriodicalId":13595,"journal":{"name":"Innovations in Microbiology and Biotechnology Vol. 1","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77769264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-30DOI: 10.9734/bpi/imb/v1/3959f
Dr. Mohiuddin Kabir
Petroleum based plastics and their derivatives that are non-biodegradable cannot be ignored at all because they have been using in every aspect of our daily life such as applications in packaging, textile, agriculture, electronics, medical, building construction, injection and moulding. Consumption of these materials are growing day-by-day that are ultimately increasing environmental pollution, global warming, and waste management cost as well as threatening the biodiversity and life on earth. Since our earth is the only planet that contains life among the countless planets in outer space, this world needs to be protected by reducing the pollution and implementing other regulatory measures. Therefore, biodegradable plastics should be used as alternative to non-biodegradable plastics. Poly-3-hydroxybutyrate (PHB) has been extensively studied and is the best-characterized biodegradable plastic within the poly-hydroxyalkanoates family. It is used to make a wide range of household and packaging products, as well as medical products. Although biodegradable PHB is environmentally friendly and does not require fossil resources, it has traditionally been exceedingly expensive to produce utilising bacterial fermentation processes involving recombinant E. coli. For efficient PHB synthesis, recombinant diatoms and transgenic plants have also been investigated. But, increasing PHB yield at the theoretical maximum level has been proved extremely difficult that prohibits its industrial scale production. To address these problems, the objective of this chapter is to focus the importance on the metabolic pathway manipulations in recombinant E. coli. The main advantage of using genetically engineered E. coli is that PHB granules are not degraded once synthesized since they lack PHB degradation pathways unlike native producers. Other benefits of employing recombinant E. coli include their capacity to (i) use a wide range of inexpensive carbon sources, (ii) accumulate huge amounts of polymers with better productivity, (iii) maintain high-cell density fermentation, and (iv) recover the PHB very easily.Since no single strategy has been proved to be sufficient enough to produce PHB industrially until today, this chapter has also shed light on developing the advanced and integrated approaches for efficient PHB production in order to compete with non-biodegradable petrochemical plastics.
{"title":"Determining the Impact of Genetic Engineering on Replacing Petroleum Based Non-Biodegradable Plastic","authors":"Dr. Mohiuddin Kabir","doi":"10.9734/bpi/imb/v1/3959f","DOIUrl":"https://doi.org/10.9734/bpi/imb/v1/3959f","url":null,"abstract":"Petroleum based plastics and their derivatives that are non-biodegradable cannot be ignored at all because they have been using in every aspect of our daily life such as applications in packaging, textile, agriculture, electronics, medical, building construction, injection and moulding. Consumption of these materials are growing day-by-day that are ultimately increasing environmental pollution, global warming, and waste management cost as well as threatening the biodiversity and life on earth. Since our earth is the only planet that contains life among the countless planets in outer space, this world needs to be protected by reducing the pollution and implementing other regulatory measures. Therefore, biodegradable plastics should be used as alternative to non-biodegradable plastics. Poly-3-hydroxybutyrate (PHB) has been extensively studied and is the best-characterized biodegradable plastic within the poly-hydroxyalkanoates family. It is used to make a wide range of household and packaging products, as well as medical products. Although biodegradable PHB is environmentally friendly and does not require fossil resources, it has traditionally been exceedingly expensive to produce utilising bacterial fermentation processes involving recombinant E. coli. For efficient PHB synthesis, recombinant diatoms and transgenic plants have also been investigated. But, increasing PHB yield at the theoretical maximum level has been proved extremely difficult that prohibits its industrial scale production. To address these problems, the objective of this chapter is to focus the importance on the metabolic pathway manipulations in recombinant E. coli. The main advantage of using genetically engineered E. coli is that PHB granules are not degraded once synthesized since they lack PHB degradation pathways unlike native producers. Other benefits of employing recombinant E. coli include their capacity to (i) use a wide range of inexpensive carbon sources, (ii) accumulate huge amounts of polymers with better productivity, (iii) maintain high-cell density fermentation, and (iv) recover the PHB very easily.Since no single strategy has been proved to be sufficient enough to produce PHB industrially until today, this chapter has also shed light on developing the advanced and integrated approaches for efficient PHB production in order to compete with non-biodegradable petrochemical plastics.","PeriodicalId":13595,"journal":{"name":"Innovations in Microbiology and Biotechnology Vol. 1","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82366920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-30DOI: 10.9734/bpi/imb/v1/4047f
S. De-Simone, Paloma Napoleão-Pêgo, L. R. Gomes, G. Lechuga, D. W. Provance-Jr
{"title":"Study on Mayaro Virus Disease: Symptoms and Treatments","authors":"S. De-Simone, Paloma Napoleão-Pêgo, L. R. Gomes, G. Lechuga, D. W. Provance-Jr","doi":"10.9734/bpi/imb/v1/4047f","DOIUrl":"https://doi.org/10.9734/bpi/imb/v1/4047f","url":null,"abstract":"","PeriodicalId":13595,"journal":{"name":"Innovations in Microbiology and Biotechnology Vol. 1","volume":"592 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77320737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-30DOI: 10.9734/bpi/imb/v1/12418d
Yu. D. Pakhomov, L. Blinkova, O. Dmítrieva, O. Berdyugina, L. Stoyanova
We studied formation of non-culturable forms of three bacteriocin (nisin) producing strains of Lactococcus lactis subsp. lactis: MSU, 729 and F-116 under carbohydrate starvation stress. Two different types of inoculum were applied: A) unwashed cells with culture liquid, B) cells washed twice with normal 0,9% saline. Resulting total numbers of cells were 0.6 1.0×108 cells/ml for both types of inoculum. Population obtained using type A inoculum demonstrated active growth phase within first 1-5 days of incubation (up to 2.4×109 cells/ml) while those obtained using type B inoculum did not grow within that period. Type B population of strain MSU showed phenotypic dissociation that resulted in appearance of micro colonies. After that, we observed active growth phase (up to 5.2×109 cells/ml). Type B cultures of strains 729 and F-116 did not grow during the whole experiment. It was shown that type A population shifted into non-culturability faster than type B. This is due to differences in metabolic strategies and stress sensitivity of these types of population. After 1 year of incubation (383 days) culturability decreased by 3 orders of magnitude for type B (5 orders for type B population of strain MSU) and by 6 orders of magnitude for type A population. We also observed considerable reduction of cell size for type A population of strains 729 and F-116. Studies of bacteriocin activity showed that in type B population cells were up to 78 times more productive compared to those of type A cultures. This phenomenon can be explained by differences in survival strategies of population that use antibacterial potential of bacteriocins for their benefit.
{"title":"Investigating of the Relationships between Non-Culturability and Nisin Production of Lactococcus lactis","authors":"Yu. D. Pakhomov, L. Blinkova, O. Dmítrieva, O. Berdyugina, L. Stoyanova","doi":"10.9734/bpi/imb/v1/12418d","DOIUrl":"https://doi.org/10.9734/bpi/imb/v1/12418d","url":null,"abstract":"We studied formation of non-culturable forms of three bacteriocin (nisin) producing strains of Lactococcus lactis subsp. lactis: MSU, 729 and F-116 under carbohydrate starvation stress. Two different types of inoculum were applied: A) unwashed cells with culture liquid, B) cells washed twice with normal 0,9% saline. Resulting total numbers of cells were 0.6 1.0×108 cells/ml for both types of inoculum. Population obtained using type A inoculum demonstrated active growth phase within first 1-5 days of incubation (up to 2.4×109 cells/ml) while those obtained using type B inoculum did not grow within that period. Type B population of strain MSU showed phenotypic dissociation that resulted in appearance of micro colonies. After that, we observed active growth phase (up to 5.2×109 cells/ml). Type B cultures of strains 729 and F-116 did not grow during the whole experiment. It was shown that type A population shifted into non-culturability faster than type B. This is due to differences in metabolic strategies and stress sensitivity of these types of population. After 1 year of incubation (383 days) culturability decreased by 3 orders of magnitude for type B (5 orders for type B population of strain MSU) and by 6 orders of magnitude for type A population. We also observed considerable reduction of cell size for type A population of strains 729 and F-116. Studies of bacteriocin activity showed that in type B population cells were up to 78 times more productive compared to those of type A cultures. This phenomenon can be explained by differences in survival strategies of population that use antibacterial potential of bacteriocins for their benefit.","PeriodicalId":13595,"journal":{"name":"Innovations in Microbiology and Biotechnology Vol. 1","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81343342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-30DOI: 10.9734/bpi/imb/v1/4268f
Shrestha Prasansah, Pokharel Nabaraj, Pant Anil Dev
Background: Bacteremia is the presence of bacteria in the blood stream. Because hemodialysis patients are immunocompromised, bacterial blood stream infection is the most common cause of death. In hemodialytic patients, blood stream infections are mainly due to Gram-positive cocci. Patients undergoing hemodialysis are especially vulnerable if the infection is caused by Staphylococcus aureus that accounts more than 8% mortality rate. Methods: A cross-sectional study was carried out at National Kidney Center of Nepal for duration of six months. Blood samples were collected from the individuals undergoing hemodialysis treatment. The collected blood samples were inoculated in brain heart infusion (BHI) biphasic medium in a blood culture bottle and incubated aerobically at 37°c for 18-24 hours (up to 1 week if necessary). It was then sub-cultured on blood agar plate where colony morphology and hemolytic reactions were observed. In order to identify the isolate organisms, a series of biochemical assays were performed. The modified Kirby Bauer technique was used to conduct the antimicrobial susceptibility test. The SPSS software was used to record and evaluate all the data collected. Results: The study comprised of a total of hundred patients. Gram-positive bacteria were found to have highest frequency (61.5%). Gram-positive bacteria such as Staphylococcus aureus (26.9%) and CoNS (88.4%) were isolated in high frequency, as were Gram-negative bacteria such as Escherichia coli (3.8%), Klebsiella pneumoniae (3.8%), and Pseudomonas aeruginosa (3.8%) were also isolated. Staphylococcus aureus was shown to be the most prevalent Gram-positive isolate among hemodialysis patients. Most of these S. aureus strains were reported to be amoxicillin resistant but cefoxitin sensitive Methicillin resistance was detected in 37.5% of the 16 S. aureus isolates. For most Gram-positive bacteria, amoxicillin was shown to be the least effective drug, whereas ciprofloxacin was found to the most effective. Conclusion: The purpose of the study is to understand the prevalence of bacteria in hemodialytic patients as well as the antibiogram of the isolates as it is the first to study about bloodstream infections in hemodialytic patients in Nepal. These results might provide a foundation to explore more about bacteriological infection study in hemodialytic patients and antibiogram.
{"title":"Study on Bacteremia in Hemodialytic Patients and Antibiotics Susceptibility Pattern","authors":"Shrestha Prasansah, Pokharel Nabaraj, Pant Anil Dev","doi":"10.9734/bpi/imb/v1/4268f","DOIUrl":"https://doi.org/10.9734/bpi/imb/v1/4268f","url":null,"abstract":"Background: Bacteremia is the presence of bacteria in the blood stream. Because hemodialysis patients are immunocompromised, bacterial blood stream infection is the most common cause of death. In hemodialytic patients, blood stream infections are mainly due to Gram-positive cocci. Patients undergoing hemodialysis are especially vulnerable if the infection is caused by Staphylococcus aureus that accounts more than 8% mortality rate. \u0000Methods: A cross-sectional study was carried out at National Kidney Center of Nepal for duration of six months. Blood samples were collected from the individuals undergoing hemodialysis treatment. The collected blood samples were inoculated in brain heart infusion (BHI) biphasic medium in a blood culture bottle and incubated aerobically at 37°c for 18-24 hours (up to 1 week if necessary). It was then sub-cultured on blood agar plate where colony morphology and hemolytic reactions were observed. In order to identify the isolate organisms, a series of biochemical assays were performed. The modified Kirby Bauer technique was used to conduct the antimicrobial susceptibility test. The SPSS software was used to record and evaluate all the data collected. \u0000Results: The study comprised of a total of hundred patients. Gram-positive bacteria were found to have highest frequency (61.5%). Gram-positive bacteria such as Staphylococcus aureus (26.9%) and CoNS (88.4%) were isolated in high frequency, as were Gram-negative bacteria such as Escherichia coli (3.8%), Klebsiella pneumoniae (3.8%), and Pseudomonas aeruginosa (3.8%) were also isolated. Staphylococcus aureus was shown to be the most prevalent Gram-positive isolate among hemodialysis patients. Most of these S. aureus strains were reported to be amoxicillin resistant but cefoxitin sensitive Methicillin resistance was detected in 37.5% of the 16 S. aureus isolates. For most Gram-positive bacteria, amoxicillin was shown to be the least effective drug, whereas ciprofloxacin was found to the most effective. \u0000Conclusion: The purpose of the study is to understand the prevalence of bacteria in hemodialytic patients as well as the antibiogram of the isolates as it is the first to study about bloodstream infections in hemodialytic patients in Nepal. These results might provide a foundation to explore more about bacteriological infection study in hemodialytic patients and antibiogram.","PeriodicalId":13595,"journal":{"name":"Innovations in Microbiology and Biotechnology Vol. 1","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87302531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-30DOI: 10.9734/bpi/imb/v1/3109f
Tejaswita Kajale, Abhay Pawar, Channapatana
The present study aims to investigate the production of biodiesel from neem oil with a view to determine its performance in Internal Combustion engine (I.C. engine). The physicochemical properties of the biodiesel produced were also studied. The world is getting modernized and industrialized day by day. As a result vehicles and engines are increasing. But energy sources used in these engines are limited and decreasing gradually. This condition necessitates the use of biodiesel as an alternate fuel for diesel engines. Biodiesel is a non-toxic, biodegradable, and renewable alternative fuel that may be used in diesel engines with very minor changes. Biodiesel could be produced from low-cost Neem seed oils. Biodiesel performance and testing is done in C.I. engine. Neem oil was extracted from neem seed by solvent extraction. Refractive index, density, viscosity, ash content, Saphonification value, iodine number was studied. Biodiesel has been prepared from NEEM oil by esterification and transesterification. It was examined for physical and chemical properties and chemical properties. HC, CO, NOx, SOx, and particulate matter was studied. The conversion of the biodiesel fuel's energy to work was equal to that from diesel fuel. The results also clearly indicate that the engine running with biodiesel and blends have higher NOx emission by up to 20%. However, the emissions of the CI engine running on neat biodiesel (B100) were reduced by up to 15%, 40% and 30% for CO, CO2 and THC emissions respectively, as compared to diesel fuel at various operating conditions. Biodiesel is linked to lower HC, CO, and particle emissions. This is likely owing to the fact that biodiesel includes around ten percent oxygen by weight, which aids in the oxidation of these combustion products in the cylinder.
{"title":"Determination of Engine Performance and Emission with Neem Oil (NOME) Based Bio-Diesel","authors":"Tejaswita Kajale, Abhay Pawar, Channapatana","doi":"10.9734/bpi/imb/v1/3109f","DOIUrl":"https://doi.org/10.9734/bpi/imb/v1/3109f","url":null,"abstract":"The present study aims to investigate the production of biodiesel from neem oil with a view to determine its performance in Internal Combustion engine (I.C. engine). The physicochemical properties of the biodiesel produced were also studied. The world is getting modernized and industrialized day by day. As a result vehicles and engines are increasing. But energy sources used in these engines are limited and decreasing gradually. This condition necessitates the use of biodiesel as an alternate fuel for diesel engines. Biodiesel is a non-toxic, biodegradable, and renewable alternative fuel that may be used in diesel engines with very minor changes. Biodiesel could be produced from low-cost Neem seed oils. Biodiesel performance and testing is done in C.I. engine. Neem oil was extracted from neem seed by solvent extraction. Refractive index, density, viscosity, ash content, Saphonification value, iodine number was studied. Biodiesel has been prepared from NEEM oil by esterification and transesterification. It was examined for physical and chemical properties and chemical properties. HC, CO, NOx, SOx, and particulate matter was studied. The conversion of the biodiesel fuel's energy to work was equal to that from diesel fuel. The results also clearly indicate that the engine running with biodiesel and blends have higher NOx emission by up to 20%. However, the emissions of the CI engine running on neat biodiesel (B100) were reduced by up to 15%, 40% and 30% for CO, CO2 and THC emissions respectively, as compared to diesel fuel at various operating conditions. Biodiesel is linked to lower HC, CO, and particle emissions. This is likely owing to the fact that biodiesel includes around ten percent oxygen by weight, which aids in the oxidation of these combustion products in the cylinder.","PeriodicalId":13595,"journal":{"name":"Innovations in Microbiology and Biotechnology Vol. 1","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90156767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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