Chemotactic Motility and Growth of Pseudomonas fluorescens Towards Glucose Concentration

Endah Rosa, U. M. Batubara, S. Suparjo
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Abstract

Pseudomonas fluorescens is plant growth promoting rhizobacteria (PGPR) often inoculated on plants as natural biocontrol agent capable of protecting the plants from soil-borne pathogens. Chemotactic motility allows populations of P. fluorescens to rapidly search for nutrients and is an important factor determining their competitive success to colonize plant root. Therefore, we investigated various glucose concentrations from 0% to 1% (w/v) to enhance chemotactic motility and growth of this rhizobia. Chemotactic motility was evaluated using swim plate assay and bacterial growth was measured using UV-Vis Spectrophotometer in LB and M9 medium. Glucose with low concentration (0.05%) showed to have optimum response in P. fluorescens chemotactic motility with colony diameter 38.3 mm in LB medium and 12.8 mm in M9 medium. Highest growth of P. fluorescens was 7 -1 seen in control condition of LB medium reaching a peak at 0.0246 OD (~±1.44x10 CFU mL ) while growth in 600 7 -1 M9 medium supplemented with 1% glucose was just slightly lower with 0.0227 OD (~±1.32x10 CFU mL ). 600 Glucose in high concentration showed to repress chemotactic motility and first growth phase of P. fluorescens in LB medium due to catabolite repression.
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荧光假单胞菌对葡萄糖浓度的趋化运动和生长
荧光假单胞菌(Pseudomonas fluorescens)是一种促进植物生长的根瘤菌,常作为天然生物防治剂接种于植物上,具有保护植物免受土传病原体侵害的作用。趋化运动使荧光假单胞菌群体能够快速寻找营养物质,是决定其在植物根部定殖竞争成功的重要因素。因此,我们研究了0%至1% (w/v)葡萄糖浓度对该根瘤菌趋化活性和生长的影响。在LB和M9培养基中采用游板法评价趋化运动性,用紫外可见分光光度计测定细菌生长。低浓度的葡萄糖(0.05%)对荧光p.h ncens趋化运动的响应最佳,在LB培养基中菌落直径为38.3 mm,在M9培养基中菌落直径为12.8 mm。在LB培养基的对照条件下,荧光假单胞菌的生长最高,达到7 -1,在0.0246 OD(~±1.44 × 10 CFU mL)时达到峰值,而在添加1%葡萄糖的600 7 -1 M9培养基中,生长略低,为0.0227 OD(~±1.32 × 10 CFU mL)。在LB培养基中,高浓度葡萄糖表现出由于分解代谢抑制而抑制荧光假单胞菌的趋化运动和第一生长期。
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