Hyperdry Human Amniotic Membrane (HD-AM) is Supporting AciclovirIncluded Device of Poly-N-p-Vinyl-Benzyl-D-Lactonamide (PVLA) Spherefor Treatment of HSV-1 Infected Rabbit Keratitis Model

M. Okabe, Toshiko Yoshida, Michiko Suzuki, M. Goto, M. Omori, M. Taguchi, Ayaka Toda, Takuma Suzuki, K. Nakagawa, Fumitaka Hiramoto, T. Ushijima, Hiroki Waki, Etsuko Furuichi, K. Arai, Kaixuan Zhou, Moustafa Fathy Omar, Makoto Nakamura, Y. Nomura, T. Kasama, Kiyoshi Katou, S. Saito, T. Nikaido
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引用次数: 15

Abstract

The human amniotic membrane (AM) has been widely used in ophthalmic surgery, including in ocular surface reconstruction. However, whether AM is effective for the release of impregnated drugs is unknown. Hyperdry-AM (HD-AM), glutaraldehyde cross-linked HD-AM (Glu HD-AM) and a solvent of poly-N-p-vinylbenzyl- D-lactonamide (PVLA) did not cause irritation to the rabbit eye. Although the concentration of residual glutaraldehyde in Glu HD-AM was approximately 40 ppm (dry-weight basis), no eye irritation was observed. Acyclovir (ACV) containing PVLA-bearing spheres (ACV/PVLA) was loaded into HD-AM (ACV/PVLA HD-AM), and then the therapeutic efficacy of ACV/PVLA HD-AM was compared with that of ACV/PVLA solution in a herpes simplex virus-1 (HSV-1) eye infection model. The former was more effective against ocular infection, and its efficacy was dose- and volume-dependent. Thus, ACV/PVLA HD-AM sheets were very effective as a curative treatment for eye infection with HSV-1. HD-AM can be function as drug delivery system. In the future, it may be possible to combine HD-AM and PVLA in a device that can control the release of hydrophobic medicines.
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超干燥人羊膜(HD-AM)支持含aciclovir的聚n -p-乙烯基苄基- d -内酯酰胺(PVLA)球治疗HSV-1感染兔角膜炎模型
人羊膜(AM)已广泛应用于眼科手术,包括眼表重建。然而,AM是否对浸渍药物的释放有效尚不清楚。超干am (HD-AM)、戊二醛交联的HD-AM (Glu HD-AM)和聚n -p-乙烯基苄基- d -内酯酰胺(PVLA)溶剂对兔眼没有刺激作用。虽然Glu HD-AM中残留戊二醛的浓度约为40 ppm(干重基础),但未观察到眼睛刺激。在单纯疱疹病毒1型(HSV-1)眼部感染模型中,将含含PVLA球(ACV/PVLA)的阿昔洛韦(ACV)装入HD-AM (ACV/PVLA HD-AM)中,比较ACV/PVLA HD-AM与ACV/PVLA溶液的治疗效果。前者对眼部感染更有效,其疗效与剂量和容量有关。因此,ACV/PVLA HD-AM片是非常有效的治疗眼部感染HSV-1的治疗方法。HD-AM可作为给药系统。在未来,有可能将HD-AM和PVLA结合在一个可以控制疏水药物释放的装置中。
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