Screening of Cellulose and Pectin Degrading Actinomycetes and Evaluation of the Ability of Composite Flora to Degrade Corn Straw

IF 0.6 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Chiang Mai Journal of Science Pub Date : 2023-05-31 DOI:10.12982/cmjs.2023.021
Xiaofei Liu, J. Lian, Zhihui Zheng, Ye Qi, Guangjian Zhang, Wei Wang, Naipu Zhang
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Abstract

Corn straw is a type of high-yield agricultural waste in China, but it has not been fully utilized owing to the diffi culty of degrading it and its low utilization rate. To improve the rate of degradation of corn straw and ensure that the straw resources are utilized more fully, we obtained an actinomycete that could simultaneously degrade cellulose and pectin from the cold region and black glebe of northeast China. The strain was named DPA-3-18, and its biological morphology and the sequence of its 16S rRNA were identifi ed. These analyses determined that it is a species of Streptomyces. The strain was combined with the lab-stored GS-30 and Bacillus subtilis to construct composite fl ora and conduct a single factor experiment. Signifi cantly higher factors were selected by Plackett Burman, and its fermentation conditions were optimized using response surface methodology. The optimal cultivation conditions were 4% of inoculum, a culture temperature of 28.51°C, an initial pH value of the medium of 7.43, and the oscillation during fermentation took place at 199.62 rpm. Under these conditions, 40.38% of the corn straw was degraded. F rom the results of SEM, we found that the structure of corn straw before degradation was complete, and the internal structure of corn straw after degradation with c omposite fl ora was destructed. The FT-IR spectra shown that the absorption peaks in all places of corn straw were reduced after degradation by the composite fl ora, the absorption peaks at 1640 cm-1 of corn straw after degradation were lower than that of before degrad ation, which confi rmed the decrease in cellulose. It indicated that the corn straw was signifi cantly degraded by the composite fl ora. This study contributed to understanding the change in corn straw after fermentation by the composite fl ora, and providing a theoretical basis for comprehensive utilization of corn straw.
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纤维素和果胶降解放线菌的筛选及复合菌群降解玉米秸秆能力的评价
玉米秸秆在中国是一种高产农业废弃物,但由于其降解难度大,利用率低,尚未得到充分利用。为了提高玉米秸秆的降解速度,保证秸秆资源的充分利用,我们获得了一种能同时降解东北寒区和黑地纤维素和果胶的放线菌。该菌株被命名为DPA-3-18,对其生物学形态和16S rRNA序列进行了鉴定,确定其为链霉菌属。将该菌株与实验室保存的GS-30和枯草芽孢杆菌联合构建复合菌群并进行单因素实验。通过Plackett Burman筛选出较高的影响因子,并利用响应面法对其发酵条件进行优化。最佳培养条件为接种量为4%,培养温度为28.51℃,培养基初始pH值为7.43,发酵过程中振荡发生在199.62 rpm。在此条件下,玉米秸秆的降解率为40.38%。从SEM的结果可以看出,降解前的玉米秸秆结构是完整的,而c复合菌降解后的玉米秸秆内部结构被破坏。FT-IR光谱显示,复合溶剂降解后玉米秸秆各部位的吸收峰均降低,降解后玉米秸秆在1640 cm-1处的吸收峰低于降解前,证实了纤维素的减少。结果表明,复合菌群对玉米秸秆降解效果显著。本研究有助于了解复合菌落发酵后玉米秸秆的变化,为玉米秸秆的综合利用提供理论依据。
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来源期刊
Chiang Mai Journal of Science
Chiang Mai Journal of Science MULTIDISCIPLINARY SCIENCES-
CiteScore
1.00
自引率
25.00%
发文量
103
审稿时长
3 months
期刊介绍: The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so.
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