Accumulation of phytoene and astaxanthin and related genes expression in Haematococcus pluvialis under sodium acetate stress

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2020-11-19 DOI:10.3354/ab00733

X. Cong, X. Zang, M. Dong, Z. Wang, B. He, L. Hou, X. Wei, F. Zhang, M. Shang, Z. Yangzong, R. Li, X. Zhang

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引用次数: 5

Abstract

Phytoene and astaxanthin are 2 important carotenoids in the green alga Haematococcus pluvialis. Under environmental stress, the synthesis of phytoene in H. pluvialis increases significantly, and phytoene is converted to astaxanthin through enzymatic catalysis. This paper analyzes the relationship between astaxanthin and phytoene accumulation in carotenoid synthesis pathways under different concentrations of sodium acetate (NaAc) by high-performance liquid chromatography. The highest concentrations of phytoene and astaxanthin were observed at the NaAc concentration of 6 g l−1 on the 12th day of induction. The highest astaxanthin concentration achieved was 2.26 ± 0.28%. Therefore, we concluded that 6 g l−1 NaAc and induction for 12 d provided the optimal inducing conditions for astaxanthin accumulation in H. pluvialis. psy, pds, lcyB, β-carotene ketolase crtw, and crtz, which are genes related to phytoene and astaxanthin synthesis, were cloned and studied at the transcriptional level. crtw and crtz were continuously up-regulated since the first day of induction, while psy, pds, and lcyB were continuously up-regulated starting on the 3rd day of induction. These findings are important for enhancing our understanding of the mechanism of accumulation of phytoene and astaxanthin in H. pluvialis and provide a foundation for identifying the induction conditions necessary for optimizing astaxanthin production and increasing astaxanthin yields.

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醋酸钠胁迫下雨红球菌植物烯和虾青素积累及相关基因表达

植物烯和虾青素是绿藻雨红球藻中两种重要的类胡萝卜素。在环境胁迫下，雨水杨中植物烯的合成显著增加，植物烯通过酶催化转化为虾青素。采用高效液相色谱法分析了不同浓度乙酸钠(NaAc)作用下类胡萝卜素合成途径中虾青素与植物烯积累的关系。诱导第12天NaAc浓度为6 g l−1时，植物烯和虾青素含量最高。最高虾青素浓度为2.26±0.28%。综上所述，6 g l−1 NaAc诱导12 d为雨水杨虾青素积累的最佳诱导条件。克隆了植物烯和虾青素合成相关基因psy、pds、lcyB、β-胡萝卜素酮化酶crtw和crtz，并在转录水平上进行了研究。crtw、crtz自诱导第1天开始持续上调，而psy、pds、lcyB自诱导第3天开始持续上调。这些发现对于进一步了解雨水杨中植物烯和虾青素的积累机制具有重要意义，并为确定优化虾青素产量和提高虾青素产量所需的诱导条件提供了基础。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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