Screening Some Extracellular Enzymes of Wild Mushrooms from Pt. Ravishankar Shukla University Campus

Abstract

Wild mushrooms are well known to produce wide range of bioactive metabolites and different types of enzymes. In this study 5 wild mushroom samples were collected which belongs to different groups. Samples were isolated and observed the culture characteristics, during the growth of mycelia many biochemical changes are known to occur, as a result of which enzymes are secreted extracellularly to degrade the insoluble materials into the substrates. Primary screening of extracellular amylase and cellulose were carried out by plate culture method in the GYP media with soluble starch to test the amylase activity and for cellulase, CMC (Carboxymethyl cellulose) plate assay was used. All the mushroom cultures differ in context of extracellular enzymatic activity. The activity of amylase enzyme was substantially higher in all the mushroom cultures. In the screening of cellulase enzyme two cultures were observed as positive. Present study suggests the capacity of these wild mushrooms in the production of biotechnologically useful enzymes with great industrial importance.