The Effect of Water Concentration on Growth Media on Lipid Production by Oleaginous Fungi Isolate BR 2.2

Herin Yoga Lesti, Miftahul Ilmi
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Abstract

Oleaginous fungi are one of the microorganisms that can accumulate a high number of biomasses quickly (within 96-130 hours) and are often used to produce lipids. The growth of fungi depends on the chemical composition of the environment in which it grows. The growth media of fungi must contain high carbohydrates as a source of nutrients and high nitrogen content. One of the carbon sources that fungi can use in the growth process is glucose. BR 2.2 isolate is an oleaginous fungus capable of accumulating 28.44% lipids from the total dry biomass with glucose as a carbon source in 50 mL of growth media. Therefore, this study was conducted to determine the effect of variations in the volume of media and incubation time on the production of biomass and lipid isolate BR 2.2. Biomass and lipid production were analyzed at media with additional water volumes of 10, 20, 30, 40, and 50 mL with 48, 96, and 144 hours of incubation times. The results showed that lipid accumulation and biomass production increased with the reduction of water content in the growth media and reached the highest number in the media volume of 20 mL with an incubation time of 144 hours, i.e., 0.87±0.04 g/L and 12.53±0.29 g/L. It can be concluded that biomass and fungal lipid increased along with incubation time and nutrient concentration.
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培养基水浓度对产油真菌br2.2产脂的影响
产油真菌是一种能够快速(在96-130小时内)积累大量生物量的微生物,通常用于生产脂质。真菌的生长取决于其生长环境的化学成分。真菌的生长培养基必须含有高碳水化合物作为营养来源和高氮含量。真菌在生长过程中可以使用的碳源之一是葡萄糖。BR 2.2分离菌是一种产油真菌,以葡萄糖为碳源,在50 mL的生长培养基中,能从总干生物量中积累28.44%的油脂。因此,本研究旨在确定培养基体积和培养时间的变化对生物质和脂质分离物br2.2产量的影响。在额外水量为10、20、30、40和50 mL的培养基上,在48、96和144小时的孵育时间下,分析生物量和脂质产量。结果表明,随着培养基含水量的降低,脂质积累和生物量增加,培养时间为144 h,在培养基体积为20 mL时达到最高,分别为0.87±0.04 g/L和12.53±0.29 g/L。生物量和真菌脂质随培养时间和营养浓度的增加而增加。
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