Mango’s mistletoe is one of the herbal plants’ rich in bioactivity. Secondary metabolites are not only produced by plants but also by microorganisms that live in tissues. One of these microorganisms is endophytic mold. The ability of endophytic molds to synthesize secondary metabolites is an opportunity for large-scale production in a short time without causing the exploitation of natural materials. This research aimed to explore and identify endophytic molds from the leaves and stem of the mango mistletoe to obtain the genus of molds. The stages of this research consisted of isolation by direct planting with sterilization, purification, growth rate measurement, characterization, and identification carried out macroscopically and microscopically. DBM 1 and DBM 2 belong to Aspergillus sp., DBM 3 belongs to Cladosporium sp., DBM 4 belongs to Neurospora sp., TDBM 1, TDBM 2, and TDBM 3 belong to Hormiscium sp. The growth rate of Aspergillus sp. relatively fast, with the increase in diameter of Aspergillus sp.1 colony from 2.45 cm to 5.05 cm and that of Aspergillus sp.2 from 2.73 cm to 5.35 cm. In the Cladosporium sp., there was an exponential phase with an increase in diameter from 2.15 cm to 4.65 cm. In Neurospora sp., there was an exponential phase with an increase in diameter from 0.63 cm to 3.65 cm. The growth rate of Hormiscium sp. is quite fast, with an exponential phase with an increase in the diameter of the colonies of Hormiscium sp.1 from 2.63 cm to 7.21 cm, Hormiscium sp.2 from 2.45 cm to 6.94 cm and Hormiscium sp.3 from 2.85 cm to 7.85 cm.
{"title":"Exploration and Identification of Endophytic Molds on Leaves and Stem of the Mango’s Mistletoe (Dendrophthoe Pentandra (L.) Miq)","authors":"Chintya Ayu Pertiwi, Nour Athiroh Abdoes Sjakoer, Nurul Jadid Mubarakati, Fatimah Fatimah","doi":"10.26740/jrba.v5n2.p79-88","DOIUrl":"https://doi.org/10.26740/jrba.v5n2.p79-88","url":null,"abstract":"Mango’s mistletoe is one of the herbal plants’ rich in bioactivity. Secondary metabolites are not only produced by plants but also by microorganisms that live in tissues. One of these microorganisms is endophytic mold. The ability of endophytic molds to synthesize secondary metabolites is an opportunity for large-scale production in a short time without causing the exploitation of natural materials. This research aimed to explore and identify endophytic molds from the leaves and stem of the mango mistletoe to obtain the genus of molds. The stages of this research consisted of isolation by direct planting with sterilization, purification, growth rate measurement, characterization, and identification carried out macroscopically and microscopically. DBM 1 and DBM 2 belong to Aspergillus sp., DBM 3 belongs to Cladosporium sp., DBM 4 belongs to Neurospora sp., TDBM 1, TDBM 2, and TDBM 3 belong to Hormiscium sp. The growth rate of Aspergillus sp. relatively fast, with the increase in diameter of Aspergillus sp.1 colony from 2.45 cm to 5.05 cm and that of Aspergillus sp.2 from 2.73 cm to 5.35 cm. In the Cladosporium sp., there was an exponential phase with an increase in diameter from 2.15 cm to 4.65 cm. In Neurospora sp., there was an exponential phase with an increase in diameter from 0.63 cm to 3.65 cm. The growth rate of Hormiscium sp. is quite fast, with an exponential phase with an increase in the diameter of the colonies of Hormiscium sp.1 from 2.63 cm to 7.21 cm, Hormiscium sp.2 from 2.45 cm to 6.94 cm and Hormiscium sp.3 from 2.85 cm to 7.85 cm.","PeriodicalId":17741,"journal":{"name":"Jurnal Riset Biologi dan Aplikasinya","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136344741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.26740/jrba.v5n2.p98-109
Risma Yulinda Putri, Ahmad Bashri
Rhizophora is a genus of mangrove plants that dominates the Banyuurip Mangrove Center. This plant certainly has an anatomical structure as a form of adaptation to high salinity environments. The purpose of this study was to describe the anatomical characteristics of the leaves of the three Rhizophora species as an adaptation form of mangrove plants in the Banyuurip Mangrove Center and the anatomical variations of the leaves between species. This type of research was descriptive observational in the form of leaf anatomy observations using the whole mount method for longitudinal incisions and the paraffin method for transverse incisions. The results showed that there were anatomical variations among the three Rhizophora species in the Banyuurip Mangrove Center including epidermal cell size, number of epidermal cells, cork warts, number of hypodermis cell layers, hypodermis cell size, stomata size, number of stomata, stomata index, and stomata density. The anatomical characteristics of the leaves of the three Rhizophora species which act as a form of adaptation to mangrove plants are the presence of hypodermis tissue which functions to store water and salt to remove salt content in plants when they abort their leaves, low density of stomata and the presence of cuticles on the adaxial surface of leaves which play a role in reducing the rate of transpiration thus maintaining water to support plant development in saline conditions, cork warts which function as a medium for expelling salt on leaves.
{"title":"Anatomical Characteristics of Rhizophora’s Leaves as Mangrove Plant Adaptation at Banyuurip Mangrove Center","authors":"Risma Yulinda Putri, Ahmad Bashri","doi":"10.26740/jrba.v5n2.p98-109","DOIUrl":"https://doi.org/10.26740/jrba.v5n2.p98-109","url":null,"abstract":"Rhizophora is a genus of mangrove plants that dominates the Banyuurip Mangrove Center. This plant certainly has an anatomical structure as a form of adaptation to high salinity environments. The purpose of this study was to describe the anatomical characteristics of the leaves of the three Rhizophora species as an adaptation form of mangrove plants in the Banyuurip Mangrove Center and the anatomical variations of the leaves between species. This type of research was descriptive observational in the form of leaf anatomy observations using the whole mount method for longitudinal incisions and the paraffin method for transverse incisions. The results showed that there were anatomical variations among the three Rhizophora species in the Banyuurip Mangrove Center including epidermal cell size, number of epidermal cells, cork warts, number of hypodermis cell layers, hypodermis cell size, stomata size, number of stomata, stomata index, and stomata density. The anatomical characteristics of the leaves of the three Rhizophora species which act as a form of adaptation to mangrove plants are the presence of hypodermis tissue which functions to store water and salt to remove salt content in plants when they abort their leaves, low density of stomata and the presence of cuticles on the adaxial surface of leaves which play a role in reducing the rate of transpiration thus maintaining water to support plant development in saline conditions, cork warts which function as a medium for expelling salt on leaves.","PeriodicalId":17741,"journal":{"name":"Jurnal Riset Biologi dan Aplikasinya","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136344215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.26740/jrba.v5n2.p70-78
Balqis Balqiah Shafie, Liew Juneng, Azman Abdul Rahim
The waters of Peninsular Malaysia located between the South China Sea and the Straits of Malacca are greatly influenced by two different monsoons: the Southwest Monsoon (SW) and the Northeast Monsoon (NE). Zooplankton are known to be receptive to any environmental changes. Apart from biotic and abiotic changes, the movement of water currents also has an impact on the distribution and composition of zooplankton, as this group is considered a passive swimmer that is dependent on the movement of water currents. This study was conducted to determine the effect of water movement in the southern waters of Peninsular Malaysia on the distribution of zooplankton. Sampling was done in three main areas: Kukup, Pengerang, and Pulau Besar. A total of 16 stations with three replicates per station were performed using a plankton net with a mesh size of 140µm. Results show that zooplankton in Pengerang recorded the highest with 256.157 ind/ m3, followed by Kukup (132.412 ind/m3) and Pulau Besar (54.066 ind/m3). There were seven dominant species of copepods recorded in this study, namely Acrocalanus gracilis, Bestiolona similis, Euterpina acutifrons, Oithona nana, Oithona similis, Paracalanus aculeatus, and Paracalanus denudatus. Generally, this study has shown a notable relationship between local current circulation patterns and zooplankton distribution.
{"title":"Effect of Water Circulation on the Distribution of Zooplankton in the Southern Waters of Peninsular Malaysia","authors":"Balqis Balqiah Shafie, Liew Juneng, Azman Abdul Rahim","doi":"10.26740/jrba.v5n2.p70-78","DOIUrl":"https://doi.org/10.26740/jrba.v5n2.p70-78","url":null,"abstract":"The waters of Peninsular Malaysia located between the South China Sea and the Straits of Malacca are greatly influenced by two different monsoons: the Southwest Monsoon (SW) and the Northeast Monsoon (NE). Zooplankton are known to be receptive to any environmental changes. Apart from biotic and abiotic changes, the movement of water currents also has an impact on the distribution and composition of zooplankton, as this group is considered a passive swimmer that is dependent on the movement of water currents. This study was conducted to determine the effect of water movement in the southern waters of Peninsular Malaysia on the distribution of zooplankton. Sampling was done in three main areas: Kukup, Pengerang, and Pulau Besar. A total of 16 stations with three replicates per station were performed using a plankton net with a mesh size of 140µm. Results show that zooplankton in Pengerang recorded the highest with 256.157 ind/ m3, followed by Kukup (132.412 ind/m3) and Pulau Besar (54.066 ind/m3). There were seven dominant species of copepods recorded in this study, namely Acrocalanus gracilis, Bestiolona similis, Euterpina acutifrons, Oithona nana, Oithona similis, Paracalanus aculeatus, and Paracalanus denudatus. Generally, this study has shown a notable relationship between local current circulation patterns and zooplankton distribution.","PeriodicalId":17741,"journal":{"name":"Jurnal Riset Biologi dan Aplikasinya","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136344947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.26740/jrba.v5n2.p52-59
Ni Kadek Prederika Sari Andayani, Iriani Setyawati, Ni Wayan Sudatri
In Indonesia, Mimosa pudica Linn. (M. pudica L.) is a wild plant (weed) from the Mimosaceace family. This plant is widely used as a traditional medicine for various types of diseases such as insomnia, acute eye inflammation, urolithiasis (urinary stones), fever, and bronchitis. This study aimed to find out the effect of M. pudica leaf extract on the histological damage of the stomach and duodenum of mice. This study used a Completely Randomized Design with 24 male mice, which were divided into control placebo (P0) given CMC-Na 0.5%; and M. pudica leaf extract doses of 200 mg/kg body weight (P1); 400 mg/kg body weight (P2); and 600 mg/kg body weight (P3). The extract was administered orally for 28 days. On day 29, the animal was dissected to collect its digestive organs. The histological preparation of the stomach and duodenum of mice was performed by using the paraffin method and Hematoxylin and Eosin staining to observe the histological damage, namely congestion, hemorrhage, epithelial cells desquamation, inflammatory cell infiltration, edema, and hyperplasia. Quantitative data were analyzed with a one-way ANOVA test and Duncan's post hoc test with the SPSS version 22. The results showed that the M. pudica leaf extract increased hemorrhage and hyperplasia damage at doses of 400 and 600 mg/kg body weight in mice’s duodenum.
在印度尼西亚，Mimosa pudica Linn。是含羞草科的一种野生植物(杂草)。这种植物被广泛用作治疗各种疾病的传统药物，如失眠、急性眼炎、尿石症、发烧和支气管炎。本研究旨在探讨苦参叶提取物对小鼠胃、十二指肠组织损伤的影响。本研究采用完全随机设计，24只雄性小鼠分为对照组(P0)，给予0.5% CMC-Na;母羊叶提取物剂量为200 mg/kg体重(P1);400 mg/kg体重(P2);600 mg/kg体重(P3)。口服提取物28天。第29天，解剖动物以收集其消化器官。采用石蜡法和苏木精、伊红染色对小鼠胃、十二指肠进行组织学准备，观察其组织损伤情况，即充血、出血、上皮细胞脱屑、炎症细胞浸润、水肿、增生。定量资料采用单因素方差分析和Duncan事后检验，采用SPSS version 22进行分析。结果表明，400和600 mg/kg体重剂量的阴藤叶提取物可增加小鼠十二指肠出血和增生损伤。
{"title":"Chronic Toxicity Effect of Mimosa pudica Leaf Extract Towards Histology Profile of Stomach and Duodenum in Mice","authors":"Ni Kadek Prederika Sari Andayani, Iriani Setyawati, Ni Wayan Sudatri","doi":"10.26740/jrba.v5n2.p52-59","DOIUrl":"https://doi.org/10.26740/jrba.v5n2.p52-59","url":null,"abstract":"In Indonesia, Mimosa pudica Linn. (M. pudica L.) is a wild plant (weed) from the Mimosaceace family. This plant is widely used as a traditional medicine for various types of diseases such as insomnia, acute eye inflammation, urolithiasis (urinary stones), fever, and bronchitis. This study aimed to find out the effect of M. pudica leaf extract on the histological damage of the stomach and duodenum of mice. This study used a Completely Randomized Design with 24 male mice, which were divided into control placebo (P0) given CMC-Na 0.5%; and M. pudica leaf extract doses of 200 mg/kg body weight (P1); 400 mg/kg body weight (P2); and 600 mg/kg body weight (P3). The extract was administered orally for 28 days. On day 29, the animal was dissected to collect its digestive organs. The histological preparation of the stomach and duodenum of mice was performed by using the paraffin method and Hematoxylin and Eosin staining to observe the histological damage, namely congestion, hemorrhage, epithelial cells desquamation, inflammatory cell infiltration, edema, and hyperplasia. Quantitative data were analyzed with a one-way ANOVA test and Duncan's post hoc test with the SPSS version 22. The results showed that the M. pudica leaf extract increased hemorrhage and hyperplasia damage at doses of 400 and 600 mg/kg body weight in mice’s duodenum.","PeriodicalId":17741,"journal":{"name":"Jurnal Riset Biologi dan Aplikasinya","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136344739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.26740/jrba.v5n2.p60-69
Bintan Felia Puspita, Wisanti Wisanti
Ferns are significantly dominant in Indonesia. The waterfall area is one of the ferns habitats. This study aims to determine the diversity index of ferns species in the Tourism Area of Sekar Pudak Sari Waterfall Wonosalam District, Jombang Regency, East Java. This research was exploratory, descriptive. Data collection uses a single plot measuring 20x20 m at 500 masl, 700 masl, and 900 masl. Each observation plot has a sub-plot with a size of 2x2 m. Data analysis used the Shannon-Wiener diversity index (H'), the taxonomic diversity index (Δ), and dominance (Δ*). The exploration results found 28 species from 11 families, 6 epiphytes, and the rest terrestrial. The taxonomic diversity index in plot 1 was 23.36 (low category), while plots 2 and 3 were 30.58 and 31.66 (medium category). This shows that the environment in the three plots is classified as stable, as indicated by the presence of ferns at various taxonomic levels. The highest dominance diversity index (Δ*) was 2.8 in Plot 2, while Plots 1 and 3 were 2.7 (low category). The total number of individual ferns found influenced the difference in the diversity index. The ferns found in the three plots were relatively few, indicating that the environment was classified as less stable. The Shannon-Wiener diversity index (H') in the three plots was 2.9 (medium category), indicating that the distribution pattern of ferns in the environment was balanced. The Sekar Pudak Sari Waterfall Tourism Area is a suitable habitat for the growth of ferns, so conservation efforts need to be made to maintain a balance of species and stabilize the ecosystem.
{"title":"Ferns Species Diversity in the Sekar Pudak Sari Waterfall Tourism Area, Wonosalam District, Jombang Regency, East Java, Indonesia","authors":"Bintan Felia Puspita, Wisanti Wisanti","doi":"10.26740/jrba.v5n2.p60-69","DOIUrl":"https://doi.org/10.26740/jrba.v5n2.p60-69","url":null,"abstract":"Ferns are significantly dominant in Indonesia. The waterfall area is one of the ferns habitats. This study aims to determine the diversity index of ferns species in the Tourism Area of Sekar Pudak Sari Waterfall Wonosalam District, Jombang Regency, East Java. This research was exploratory, descriptive. Data collection uses a single plot measuring 20x20 m at 500 masl, 700 masl, and 900 masl. Each observation plot has a sub-plot with a size of 2x2 m. Data analysis used the Shannon-Wiener diversity index (H'), the taxonomic diversity index (Δ), and dominance (Δ*). The exploration results found 28 species from 11 families, 6 epiphytes, and the rest terrestrial. The taxonomic diversity index in plot 1 was 23.36 (low category), while plots 2 and 3 were 30.58 and 31.66 (medium category). This shows that the environment in the three plots is classified as stable, as indicated by the presence of ferns at various taxonomic levels. The highest dominance diversity index (Δ*) was 2.8 in Plot 2, while Plots 1 and 3 were 2.7 (low category). The total number of individual ferns found influenced the difference in the diversity index. The ferns found in the three plots were relatively few, indicating that the environment was classified as less stable. The Shannon-Wiener diversity index (H') in the three plots was 2.9 (medium category), indicating that the distribution pattern of ferns in the environment was balanced. The Sekar Pudak Sari Waterfall Tourism Area is a suitable habitat for the growth of ferns, so conservation efforts need to be made to maintain a balance of species and stabilize the ecosystem.","PeriodicalId":17741,"journal":{"name":"Jurnal Riset Biologi dan Aplikasinya","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136344214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.26740/jrba.v5n2.p89-97
Siti Amira, Husnarika Febriani, Syukriah Syukriah, Ulinnuha Nur Faizah
Sodium nitrite (NaNO2) is one of the most inorganic salts and is often used in processed meat products. Long-term consumption of sodium nitrite has been reported to cause of oxidative stress and spleen histological changes. Therefore, anti-oxidative effects are needed from natural ingredients such as Acorus calamus L. The study aims to determine the effect of A.calamus L. rhizome extract on anatomical structure and histological changes in the rat spleen induced with NaNO2. We used a completely randomized design with twenty-five male rats divided into 5 groups. Negative control (NC) was given 0.5% CMC for 40 days; positive control (PC) on days 1-15 was given NaNO2 (50 mg/kg BW); on days 16-30 given (50 mg/kg BW) NaNO2 + 0.5% CMC and on days 31-40 given 0,5% carboxy methyl cellulose (CMC). Treatment 1 (T1), Treatment 2 (T2), and Treatment 3 (T3) on days 1-15 were given NaNO2 doses of 50 mg; and; on days 16-30, groups T1, T2, and T3 were given NaNO2 + extract with each predetermined dose (250 mg/kg BW for T1, 500 mg/kg BW for T2, and 750 mg/kg BW for T3). For days 31-40 groups T1, T2, and T3 were given extracts with each predetermined dose. Results revealed that the extract of the A. calamus L. rhizome affects weight, length, and width of the spleen (P<0.05); and bleeding lesions; while having no noticeable effect (P<0.05) and having a noticeable effect on the area of the white pulp (P<0.05). Administration of ethanol extract of A. calamus L. at a dose of 250 mg/kg BW has an impact on improving spleen length; a dose of 500 mg/kg BW has an impact on the weight and length of the spleen; and a dose of 750 mg / kg BW has an impact on improving spleen length, but the size variation is still in the normal category. While extracts at doses of 250 and 500 mg/kg BW have a repair impact on tissue shrinkage in damaged white pulp.
{"title":"Morpho-histological Study of the Rat Spleen Post-Induction of Sodium Nitrite and Acorus calamus L. Rhizome Ethanolic Extract","authors":"Siti Amira, Husnarika Febriani, Syukriah Syukriah, Ulinnuha Nur Faizah","doi":"10.26740/jrba.v5n2.p89-97","DOIUrl":"https://doi.org/10.26740/jrba.v5n2.p89-97","url":null,"abstract":"Sodium nitrite (NaNO2) is one of the most inorganic salts and is often used in processed meat products. Long-term consumption of sodium nitrite has been reported to cause of oxidative stress and spleen histological changes. Therefore, anti-oxidative effects are needed from natural ingredients such as Acorus calamus L. The study aims to determine the effect of A.calamus L. rhizome extract on anatomical structure and histological changes in the rat spleen induced with NaNO2. We used a completely randomized design with twenty-five male rats divided into 5 groups. Negative control (NC) was given 0.5% CMC for 40 days; positive control (PC) on days 1-15 was given NaNO2 (50 mg/kg BW); on days 16-30 given (50 mg/kg BW) NaNO2 + 0.5% CMC and on days 31-40 given 0,5% carboxy methyl cellulose (CMC). Treatment 1 (T1), Treatment 2 (T2), and Treatment 3 (T3) on days 1-15 were given NaNO2 doses of 50 mg; and; on days 16-30, groups T1, T2, and T3 were given NaNO2 + extract with each predetermined dose (250 mg/kg BW for T1, 500 mg/kg BW for T2, and 750 mg/kg BW for T3). For days 31-40 groups T1, T2, and T3 were given extracts with each predetermined dose. Results revealed that the extract of the A. calamus L. rhizome affects weight, length, and width of the spleen (P<0.05); and bleeding lesions; while having no noticeable effect (P<0.05) and having a noticeable effect on the area of the white pulp (P<0.05). Administration of ethanol extract of A. calamus L. at a dose of 250 mg/kg BW has an impact on improving spleen length; a dose of 500 mg/kg BW has an impact on the weight and length of the spleen; and a dose of 750 mg / kg BW has an impact on improving spleen length, but the size variation is still in the normal category. While extracts at doses of 250 and 500 mg/kg BW have a repair impact on tissue shrinkage in damaged white pulp.","PeriodicalId":17741,"journal":{"name":"Jurnal Riset Biologi dan Aplikasinya","volume":"162 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136344216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gayam (Inocarpus fagifer) is one of the members of the angiosperm flora in Ambon City, Indonesia, that grows and develops at various altitudes. This research aimed to analyze the leaf and stomata morphometrics of these plants in the Aer Louw and Ema Village areas. Leaf samples were taken from the upper, middle, and lower strata and considered as replicates. The morphometric characteristics were measured manually using millimeter block paper and the formula for calculating leaf ratio. Furthermore, the stomata were stained using the direct incision method and safranin. The incision results were analyzed using an Olympus CX23 microscope at 400x magnification. The measurement and observation were analyzed descriptively and correlatively. The results showed that the average leaf width and length, also the midrib length were greater in Aer Louw Village than in Ema Village; while the leaf tip and stalk length were greater in Ema Village than in Aer Louw Village. The characteristics of stomata length and width in Ema Village were greater than in Aer Louw Village; otherwise, the number, index, and density of stomata in Aer Louw Village were greater than in Ema Village. Meanwhile, the correlational analysis showed that the environment influenced the variations of leaves and stomata. Therefore, the variations of leaves and stomata in the areas could predict plant adaptations to different environments.
{"title":"Leaf and Stomata Morphometrics of Gayam Inocarpus fagifer (Fabaceae) at Different Altitudes","authors":"Alwi Smith, Kristin Sangur, Dessy Fitri Molle, Ludia Haurissa, Grisendy Maulany, Belsefren Renyaan","doi":"10.26740/jrba.v5n1.p16-26","DOIUrl":"https://doi.org/10.26740/jrba.v5n1.p16-26","url":null,"abstract":"Gayam (Inocarpus fagifer) is one of the members of the angiosperm flora in Ambon City, Indonesia, that grows and develops at various altitudes. This research aimed to analyze the leaf and stomata morphometrics of these plants in the Aer Louw and Ema Village areas. Leaf samples were taken from the upper, middle, and lower strata and considered as replicates. The morphometric characteristics were measured manually using millimeter block paper and the formula for calculating leaf ratio. Furthermore, the stomata were stained using the direct incision method and safranin. The incision results were analyzed using an Olympus CX23 microscope at 400x magnification. The measurement and observation were analyzed descriptively and correlatively. The results showed that the average leaf width and length, also the midrib length were greater in Aer Louw Village than in Ema Village; while the leaf tip and stalk length were greater in Ema Village than in Aer Louw Village. The characteristics of stomata length and width in Ema Village were greater than in Aer Louw Village; otherwise, the number, index, and density of stomata in Aer Louw Village were greater than in Ema Village. Meanwhile, the correlational analysis showed that the environment influenced the variations of leaves and stomata. Therefore, the variations of leaves and stomata in the areas could predict plant adaptations to different environments.","PeriodicalId":17741,"journal":{"name":"Jurnal Riset Biologi dan Aplikasinya","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91007759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Indonesia is one of mega biodiverse countries in the world, with a high global biodiversity index and many vascular plant species. However, not all plant species are known in terms of their uses, potential benefits, and conservation status. One of example is genus Etlingera, where further studies are required. Therefore, this study aimed to provide information on the potential benefits and uses of the genus Etlingera and to investigate its conservation status. The study was conducted using the method of literature study, an inventory of potential uses, and an inventory of the genus Etlingera living in Bogor Botanic Gardens through direct observation. Six species of the genus Etlingera were collected from the Bogor Botanic Gardens, namely Etlingera brevilabrum, E. elatior, E. hemisphaerica, E. loerzingii, E. megalocheilos, and E. walang, conservation status of three species (E. brevilabrum, E. hemisphaerica, and E. megalocheilos) is Least Concern (LC), two species (E. elatior and E. walang) are Data Deficient (DD), and one species (E. loerzingii) is Vulnerable (VU). All species are commonly used as spices, condiments, cosmetics, and traditional medicine to cure various diseases, possibly also as ornamental plants. The secondary metabolites present in some species, namely E. brevilabrum, E. elatior, and E. hemisphaerica can be used as antimicrobial, antifungal, and antioxidant agents.
{"title":"Etlingera (Zingiberaceae) in Bogor Botanic Gardens: Potential Benefits and Its Conservation Status","authors":"Yeyen Novitasari","doi":"10.26740/jrba.v5n1.p1-7","DOIUrl":"https://doi.org/10.26740/jrba.v5n1.p1-7","url":null,"abstract":"Indonesia is one of mega biodiverse countries in the world, with a high global biodiversity index and many vascular plant species. However, not all plant species are known in terms of their uses, potential benefits, and conservation status. One of example is genus Etlingera, where further studies are required. Therefore, this study aimed to provide information on the potential benefits and uses of the genus Etlingera and to investigate its conservation status. The study was conducted using the method of literature study, an inventory of potential uses, and an inventory of the genus Etlingera living in Bogor Botanic Gardens through direct observation. Six species of the genus Etlingera were collected from the Bogor Botanic Gardens, namely Etlingera brevilabrum, E. elatior, E. hemisphaerica, E. loerzingii, E. megalocheilos, and E. walang, conservation status of three species (E. brevilabrum, E. hemisphaerica, and E. megalocheilos) is Least Concern (LC), two species (E. elatior and E. walang) are Data Deficient (DD), and one species (E. loerzingii) is Vulnerable (VU). All species are commonly used as spices, condiments, cosmetics, and traditional medicine to cure various diseases, possibly also as ornamental plants. The secondary metabolites present in some species, namely E. brevilabrum, E. elatior, and E. hemisphaerica can be used as antimicrobial, antifungal, and antioxidant agents.","PeriodicalId":17741,"journal":{"name":"Jurnal Riset Biologi dan Aplikasinya","volume":"87 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135897335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-31DOI: 10.26740/jrba.v5n1.p43-51
Desi Kartikasari, Gading Anon Widodo, Nur Habibah, Rakhmi Zahratul Asna
Moss plants (Bryophyta) are found in every habitat, and their presence in an ecosystem is controlled by environmental circumstances. The Senggani Ravine tourism area is a popular tourist attraction comprising a pine forest with extensive moss communities. This work aimed to assess the diversity of mosses (Bryophyta) in the Senggani Ravine tourism area for the first time. In June 2022, exploratory descriptive research of moss diversity was undertaken by a free walk around the Senggani Ravine tourism area from a predetermined position point (purposive sampling) using observation, documentation, literature study, and measurement of abiotic elements for data collection. Based on the results, twenty species of mosses were identified and can be divided into four classes, namely the Bryopsida, Polytrichopsida, Jungermanniopsida, and Marchantiopsida. The species identified were Barbulla indica, Fissidens purpusillus, Fissidens biformis, Fissidens biformis, Octoblepharum albidum, Rhizonium punctatum, Mnium hornum, Philonotis marchica, Fontinalis antipyretica, Hypnum cupressiform, Polytrichastrum formosum, Lejeunea flava, Lejeunea cavifolia, Bazzania prareupta, Bazzania vittata, Riccia junghuhniana, Marchantia emarginata, Marchantia polymorpha, Dumortiera hirsuta, and Lunularia cruciate. Abiotic factor measurements revealed that zone 3, which has a soil pH of 6, an air temperature of 24.1 C°, an 84% humidity level, and 200 Cd of light cm-1, is the most favorable area for moss growth. We can infer that the Senggani Ravine tourism area is still primarily undisturbed because the moss flora is still quite diverse and varied.
{"title":"Diversity of Moss Species (Bryophyta) In Senggani Ravine Tourism Area, Tulungagung Regency","authors":"Desi Kartikasari, Gading Anon Widodo, Nur Habibah, Rakhmi Zahratul Asna","doi":"10.26740/jrba.v5n1.p43-51","DOIUrl":"https://doi.org/10.26740/jrba.v5n1.p43-51","url":null,"abstract":"Moss plants (Bryophyta) are found in every habitat, and their presence in an ecosystem is controlled by environmental circumstances. The Senggani Ravine tourism area is a popular tourist attraction comprising a pine forest with extensive moss communities. This work aimed to assess the diversity of mosses (Bryophyta) in the Senggani Ravine tourism area for the first time. In June 2022, exploratory descriptive research of moss diversity was undertaken by a free walk around the Senggani Ravine tourism area from a predetermined position point (purposive sampling) using observation, documentation, literature study, and measurement of abiotic elements for data collection. Based on the results, twenty species of mosses were identified and can be divided into four classes, namely the Bryopsida, Polytrichopsida, Jungermanniopsida, and Marchantiopsida. The species identified were Barbulla indica, Fissidens purpusillus, Fissidens biformis, Fissidens biformis, Octoblepharum albidum, Rhizonium punctatum, Mnium hornum, Philonotis marchica, Fontinalis antipyretica, Hypnum cupressiform, Polytrichastrum formosum, Lejeunea flava, Lejeunea cavifolia, Bazzania prareupta, Bazzania vittata, Riccia junghuhniana, Marchantia emarginata, Marchantia polymorpha, Dumortiera hirsuta, and Lunularia cruciate. Abiotic factor measurements revealed that zone 3, which has a soil pH of 6, an air temperature of 24.1 C°, an 84% humidity level, and 200 Cd of light cm-1, is the most favorable area for moss growth. We can infer that the Senggani Ravine tourism area is still primarily undisturbed because the moss flora is still quite diverse and varied.","PeriodicalId":17741,"journal":{"name":"Jurnal Riset Biologi dan Aplikasinya","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84600840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-31DOI: 10.26740/jrba.v5n1.p37-42
Laily Isnaini Rahmawati, A. Basith, Fitria Lestari
This study focused on the application of microsatellite markers at the INRA032 locus for genetic diversity assessment in buffalo (Bubalus bubalis) populations in Pacitan and Tuban Regencies, East Java, Indonesia. The total number of samples used was 16, each population represented by 8 samples. Genetic diversity assessment parameters include allele frequency, the Polymorphism Information Content (PIC) and heterozygosity. The results showed that based on the INRA032 locus, the Tuban buffalo population had a higher allele frequency range (0.08 to 0.33) than the Pacitan population (0.18 to 0.31). The average PIC value in both populations was 0.39, so it can be concluded that the INRA032 locus is informative enough to detect polymorphisms in both populations. The percentage heterozygosity of the Pacitan buffalo population is 88%, which is higher than the Tuban population at 50%, suggesting that the genetic diversity of the two populations is still quite high despite the decreasing trend in population numbers.
{"title":"Genetic Profile of Local Buffalo (Bubalus bubalis) Populations in Pacitan and Tuban, East Java, Indonesia Measured by the Molecular Marker of INRA032 Locus","authors":"Laily Isnaini Rahmawati, A. Basith, Fitria Lestari","doi":"10.26740/jrba.v5n1.p37-42","DOIUrl":"https://doi.org/10.26740/jrba.v5n1.p37-42","url":null,"abstract":"This study focused on the application of microsatellite markers at the INRA032 locus for genetic diversity assessment in buffalo (Bubalus bubalis) populations in Pacitan and Tuban Regencies, East Java, Indonesia. The total number of samples used was 16, each population represented by 8 samples. Genetic diversity assessment parameters include allele frequency, the Polymorphism Information Content (PIC) and heterozygosity. The results showed that based on the INRA032 locus, the Tuban buffalo population had a higher allele frequency range (0.08 to 0.33) than the Pacitan population (0.18 to 0.31). The average PIC value in both populations was 0.39, so it can be concluded that the INRA032 locus is informative enough to detect polymorphisms in both populations. The percentage heterozygosity of the Pacitan buffalo population is 88%, which is higher than the Tuban population at 50%, suggesting that the genetic diversity of the two populations is still quite high despite the decreasing trend in population numbers.","PeriodicalId":17741,"journal":{"name":"Jurnal Riset Biologi dan Aplikasinya","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82158070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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