Ticari Meyve Sularında HPLC ile Şeker Analizi için Standart Bir Metot Teyidi

Tuğba Doğan, Tamay Şeker
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Abstract

This study aimed to verify the standard test method TS EN 12630 and determine the sugar contents of commercially available fruit juices by high pressure liquid chromatography. A laboratory should verify the standard test method parameters in order to show its performance for the analysis, under consideration. For this purpose, Sucrose, Glucose and Fructose were analysed in orange juices obtained from the market. The principle of the method is based on the separation of sugars on a cation-exchange resin by isocratic elution with mobile phase, detection using a differential refractive index (RI) detector and external standard method. Accuracy and precision were performed via intraday and inter day studies to determine of accuracy and the precision (generally accepted as repeatability and reproducibility) for the standard test method. The recovery values of the sugars added into juice sample were 92%, 99% and 96% for sucrose, glucose and fructose, respectively.
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本研究旨在验证标准测试方法TS EN 12630,并采用高压液相色谱法测定市售果汁中的糖含量。实验室应验证标准测试方法参数,以显示其对所考虑的分析的性能。为此,我们分析了从市场上获得的橙汁中的蔗糖、葡萄糖和果糖。该方法的原理是通过流动相等密度洗脱、差示折射率(RI)检测器和外标法在阳离子交换树脂上分离糖。准确度和精密度通过日间和日间研究来确定标准测试方法的准确度和精密度(通常被认为是可重复性和可再现性)。添加到果汁样品中的糖对蔗糖、葡萄糖和果糖的回收率分别为92%、99%和96%。
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