Optimum conditions for cellulose production by bacteria isolated from local apple in Egypt

Abstract

Cellulose-producing bacteria were isolated from different sources as rotten fruits, rotten vegetables and soil samples. The most efficient cellulose-producing bacteria was isolated from rotten local apple and identified as Komagataeibacter hansenii (MH1) by biochemical, morphological and 16S rRNA testes. Different media for bacterial cellulose (BC) production were tested and the most efficient one was glucose ethanol acetic acid medium (GAM medium). Optimization of nutritional and environmental conditions for bacterial cellulose production were studied. The best conditions for bacterial cellulose biosynthesis by Komagataeibacter hansenii (MH1) were 20 g/l sucrose, 10 g/l peptone, 4 ml/l acetic acid, 7ml/l ethanol at 30°C, pH 3.46 for 15 days under static culture with 5.86 g/l bacterial cellulose production. The isolated strain Komagataeibacter hansenii (MH1) was found to produce cellulose on low cost raw material begasse as it can use it as carbon source with 1.71 g/l dry BC and use it also as carbon+ nitrogen source with 1.41g/l dry BC for bacterial cellulose production but the amount of BC produced was lower than that produced on modified GAM medium.