Pub Date : 2023-01-01DOI: 10.5455/egyjebb.20230612120612
Anwer Badry, Reham Shohoud, Maha M. Azab
Twenty locations of different plant and soil samples were surveyed for microorganisms of cellulolytic activity. A total of 20 bacterial and 20 fungal isolates were collected, cultivated, purified and tested for cellulose degradation. The most efficient cellulase producers were the fungal and bacterial isolates from grape leaves; which were identified as Aspergillus flavus MH591448 and Pseudomonas aeruginosa KX610179. A more detailed study for optimization of growth conditions for the highest cellulase activity was performed for the higher cellulose-degrading isolates, namely: Aspergillus flavus, Fusarium oxysporum, Pseudomonas aeruginosa and Klebsiella aerogenes. The maximum growth and cellulolytic activity of the tested microorganisms was obtained for cultures on minimal salt nutrition medium, incubations temperature of 35°C, with initial pH value = 6, by adding ammonium sulfate as a supporting nitrogen source, without additive co-carbon source, after incubation for 2 days for bacterial isolates and 5 days for fungal isolates on a shaking incubator of 80 rpm.
{"title":"Screening and optimization of cellulolytic activity of local microbial isolates in the Egyptian Delta region.","authors":"Anwer Badry, Reham Shohoud, Maha M. Azab","doi":"10.5455/egyjebb.20230612120612","DOIUrl":"https://doi.org/10.5455/egyjebb.20230612120612","url":null,"abstract":"Twenty locations of different plant and soil samples were surveyed for microorganisms of cellulolytic activity. A total of 20 bacterial and 20 fungal isolates were collected, cultivated, purified and tested for cellulose degradation. The most efficient cellulase producers were the fungal and bacterial isolates from grape leaves; which were identified as Aspergillus flavus MH591448 and Pseudomonas aeruginosa KX610179. A more detailed study for optimization of growth conditions for the highest cellulase activity was performed for the higher cellulose-degrading isolates, namely: Aspergillus flavus, Fusarium oxysporum, Pseudomonas aeruginosa and Klebsiella aerogenes. The maximum growth and cellulolytic activity of the tested microorganisms was obtained for cultures on minimal salt nutrition medium, incubations temperature of 35°C, with initial pH value = 6, by adding ammonium sulfate as a supporting nitrogen source, without additive co-carbon source, after incubation for 2 days for bacterial isolates and 5 days for fungal isolates on a shaking incubator of 80 rpm.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"2013 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74083636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5455/egyjebb.20221223094631
A. Atawy, M. Rizk, M. Ahmed
ABSTRACT: Dehydrins have a key role in protecting plants, especially in the grey mangrove tree Avicennia marina (Forssk.) Vierh. from salt stress. Understanding the mangrove plants at the molecular level will be necessary for developing such highly salt-tolerant crops. The full-length cDNA of the DHN gene sequence AmDHN was isolated from Avicennia marina and contained a 588bp open reading frame (ORF) encoding a 195 amino acid protein with a molecular weight of about 19.746k Da. As well as, multiple alignment sequences and phylogenetic relationships were analyzed for sequences using MEGA7 software revealing that AmDHN has a high identity with other DHNs plants and suggesting that AmDHN belongs to group II (LEA) proteins. Using bioinformatics analysis tools, we have investigated characterize and gene expression to better understand the structure and function analysis prediction of the AmDHN protein. AmDHN protein contains motifs (SYK2), Sn, Yn segment, and at least one copy of a lysine-rich conserved sequence known as K-segment (consensus EKKGIME/DKIKEKLPG) near the C terminal, the 3D predicted the structure protein sequence of the secondary structure, the top 5 final models using by I-TASSER server and several parameters computed by the software were obtained. Our results indicate that the AmDHN gene plays an essential role in salt stress remediation in the Avicennia marina. They could be used for further studies to understand the molecular mechanisms of salinity tolerance in plants and can be potentially utilized in transforming other plants to improve tolerance to salinity stress.
{"title":"Isolation and Characterization of Dehydrin Gene from Egyptian Gray Mangrove in Nabq Protectorate","authors":"A. Atawy, M. Rizk, M. Ahmed","doi":"10.5455/egyjebb.20221223094631","DOIUrl":"https://doi.org/10.5455/egyjebb.20221223094631","url":null,"abstract":"ABSTRACT: Dehydrins have a key role in protecting plants, especially in the grey mangrove tree Avicennia marina (Forssk.) Vierh. from salt stress. Understanding the mangrove plants at the molecular level will be necessary for developing such highly salt-tolerant crops. The full-length cDNA of the DHN gene sequence AmDHN was isolated from Avicennia marina and contained a 588bp open reading frame (ORF) encoding a 195 amino acid protein with a molecular weight of about 19.746k Da. As well as, multiple alignment sequences and phylogenetic relationships were analyzed for sequences using MEGA7 software revealing that AmDHN has a high identity with other DHNs plants and suggesting that AmDHN belongs to group II (LEA) proteins. Using bioinformatics analysis tools, we have investigated characterize and gene expression to better understand the structure and function analysis prediction of the AmDHN protein. AmDHN protein contains motifs (SYK2), Sn, Yn segment, and at least one copy of a lysine-rich conserved sequence known as K-segment (consensus EKKGIME/DKIKEKLPG) near the C terminal, the 3D predicted the structure protein sequence of the secondary structure, the top 5 final models using by I-TASSER server and several parameters computed by the software were obtained. Our results indicate that the AmDHN gene plays an essential role in salt stress remediation in the Avicennia marina. They could be used for further studies to understand the molecular mechanisms of salinity tolerance in plants and can be potentially utilized in transforming other plants to improve tolerance to salinity stress.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"282 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77193152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5455/egyjebb.20230107122430
A. Shokr, M. Eweis, Wael Elrady, M. Rizk
The microbial composition of olive mill wastewater (OMWW) from six disposal ponds at three localities in North Sinai Governorate, Egypt has been studied. Such OMWW samples contained a variable (but high) number of bacteria, yeasts, and mold. Eighty-one isolates related to 9 bacterial species were recovered, and seven common bacterial species were isolated from Bir al Abd, Al-Arish, and Rafah sites sources namely Enterococcus faecalis, Escherichia coli, Bacillus cereus, B. subtilis, Micrococcus luteus, Pseudomonas aeruginosa, and Klebsiella pneumonia. In addition to these species, Vibrio cholerae and Shigella species were isolated from the Bir al Abd site. One hundred and twenty isolates related to 13 fungal species were recovered. Seven members of different fungal genera (Aspergillus, Penicillium, Fusarium, Paecilomyces, Alternaria, Chaetomium, and Rhizopus) were recorded. Four genera were widely distributed, and they were able to grow efficiently in undiluted OMWW as a sole source of nutrients. Strains of Aspergillus, Penicillium, Fusarium, and Paecilomyces showed a marked capacity for OMWW detoxification. The physicochemical studies further revealed the presence of relatively low levels of sodium, potassium, calcium, magnesium, and iron. Nitrate concentration was relatively high, 70.13 mg/L. The biological oxygen demand (BOD) and the chemical oxygen demand (COD) recorded high values that ranged between 2210-2270 mg/L and 22760-22800 mg/L, respectively. High BOD and COD values are important indicators of high organic and inorganic components of OMWW and possible negative environmental consequences. The presence of fungal and bacterial species implied possible degradation of the effluent.
研究了埃及北西奈省三个地区六个处理池橄榄厂废水(OMWW)的微生物组成。这样的OMWW样品含有数量可变(但很高)的细菌、酵母菌和霉菌。从Bir al Abd、al - arish和Rafah 3个站点分离到粪肠球菌、大肠埃希菌、蜡样芽孢杆菌、枯草芽孢杆菌、黄体微球菌、铜绿假单胞菌和肺炎克雷伯菌等7种常见细菌，共分离到9种细菌81株。除了这些种外，还从Bir al Abd地点分离出霍乱弧菌和志贺氏菌种。共分离到13种真菌120株。记录了不同真菌属的7个成员(曲霉、青霉、镰刀菌、拟青霉、互交菌、毛菌和根霉)。4个属分布广泛，作为唯一的营养来源，它们能够在未稀释的OMWW中高效生长。曲霉、青霉、镰刀菌和拟青霉对OMWW的解毒能力显著。物理化学研究进一步揭示了相对低水平的钠、钾、钙、镁和铁的存在。硝酸盐浓度较高，为70.13 mg/L。生物需氧量(BOD)和化学需氧量(COD)分别为2210 ~ 2270 mg/L和22760 ~ 22800 mg/L。高BOD和COD值是OMWW中高有机和无机成分以及可能产生的负面环境后果的重要指标。真菌和细菌种类的存在意味着废水可能被降解。
{"title":"A study on microbiota isolated from olive mill wastewater disposal ponds, with emphasis on filamentous fungi and their biodegradative potential in North Sinai Governorate, Egypt","authors":"A. Shokr, M. Eweis, Wael Elrady, M. Rizk","doi":"10.5455/egyjebb.20230107122430","DOIUrl":"https://doi.org/10.5455/egyjebb.20230107122430","url":null,"abstract":"The microbial composition of olive mill wastewater (OMWW) from six disposal ponds at three localities in North Sinai Governorate, Egypt has been studied. Such OMWW samples contained a variable (but high) number of bacteria, yeasts, and mold. Eighty-one isolates related to 9 bacterial species were recovered, and seven common bacterial species were isolated from Bir al Abd, Al-Arish, and Rafah sites sources namely Enterococcus faecalis, Escherichia coli, Bacillus cereus, B. subtilis, Micrococcus luteus, Pseudomonas aeruginosa, and Klebsiella pneumonia. In addition to these species, Vibrio cholerae and Shigella species were isolated from the Bir al Abd site. One hundred and twenty isolates related to 13 fungal species were recovered. Seven members of different fungal genera (Aspergillus, Penicillium, Fusarium, Paecilomyces, Alternaria, Chaetomium, and Rhizopus) were recorded. Four genera were widely distributed, and they were able to grow efficiently in undiluted OMWW as a sole source of nutrients. Strains of Aspergillus, Penicillium, Fusarium, and Paecilomyces showed a marked capacity for OMWW detoxification. The physicochemical studies further revealed the presence of relatively low levels of sodium, potassium, calcium, magnesium, and iron. Nitrate concentration was relatively high, 70.13 mg/L. The biological oxygen demand (BOD) and the chemical oxygen demand (COD) recorded high values that ranged between 2210-2270 mg/L and 22760-22800 mg/L, respectively. High BOD and COD values are important indicators of high organic and inorganic components of OMWW and possible negative environmental consequences. The presence of fungal and bacterial species implied possible degradation of the effluent.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83470647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5455/egyjebb.20230119022822
H. Sheikh
Staphylococcus aureus and Salmonella typhi are foodborne pathogens that can cause serious illnesses if ingested, such as fever, nausea, vomiting, stomach cramps and diarrhea. Therefore, it’s important to find new natural antibacterial agents to face such pathogenic bacteria. The antibacterial activity of organic extract of some medicinal plants collected from Jeddah, Saudi Arabia was tested against two pathogenic bacteria Staphylococcus aureus and Salmonella typhi. The extracts were prepared using ethanol, methanol, diethyl ether and petroleum ether. Methanol was the greatest efficient solvent for extracting the antibacterial compounds from tested plants. Syzygium aromaticum showed the highest antibacterial effect followed by Rosmarinus officinalis, Cinnamon umverum, Commiphora myrrha, Brassica nigra, Curcuma longa, Ferula assa-foetida and Alpinia officinarum. The MICs of the extracts recorded were variety from 0.5 to 4.0 mg/mL.
{"title":"Invitro antibacterial activity of some medicinal plants against food poisoning Staphylococcus aureus and Salmonella typhi","authors":"H. Sheikh","doi":"10.5455/egyjebb.20230119022822","DOIUrl":"https://doi.org/10.5455/egyjebb.20230119022822","url":null,"abstract":"Staphylococcus aureus and Salmonella typhi are foodborne pathogens that can cause serious illnesses if ingested, such as fever, nausea, vomiting, stomach cramps and diarrhea. Therefore, it’s important to find new natural antibacterial agents to face such pathogenic bacteria. The antibacterial activity of organic extract of some medicinal plants collected from Jeddah, Saudi Arabia was tested against two pathogenic bacteria Staphylococcus aureus and Salmonella typhi. The extracts were prepared using ethanol, methanol, diethyl ether and petroleum ether. Methanol was the greatest efficient solvent for extracting the antibacterial compounds from tested plants. Syzygium aromaticum showed the highest antibacterial effect followed by Rosmarinus officinalis, Cinnamon umverum, Commiphora myrrha, Brassica nigra, Curcuma longa, Ferula assa-foetida and Alpinia officinarum. The MICs of the extracts recorded were variety from 0.5 to 4.0 mg/mL.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"104 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87754557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5455/egyjebb.20230131123212
S. Teleb, Rajaa Mabrouk, Marwa M. Eldemerdash
The morphological structures of pollens grains of six taxa belonging to 3 genera of Silenoideae, Caryophyllaceae were examined with the light and scanning electron microscope . In order to elucidate its significance in taxonomy of the group, qualitative and quantitative variables of pollen characters related to the shape, size, aperture characteristics of pores, exine characteristics include exine thickness columellae pattern (LM), tectum (LM) and Sculpture of pollen grain were studied. The pollen grains in the studied species were apolar, polypantoporate, with 12-36 pores. The dominant character of pollen ornamentation is spinulose with variably sparse spinules. Pollen size also varies among different species, and length of the grain diameter are in medium-sized (23-60μm). Cluster and principal component analysis (PCA) of qualitative and quantitative data were used to demonstrate the pollen grains similarities among the species. UPGMA tree derived from cluster analysis reveals two major clusters, the first of which consists of three species (Silene dioca, Silene vulgaris subsp. thorei and Silene pusilla. The second cluster comprises the rest of species Silene vulgaris subsp. bosniaca, Dianthus gratianopolitanus and Minuartia mutabilis, a more pollen grains description to be obtained results in comparative manner to these taxa which belong the family.
{"title":"Comparative Morphological Structures of Pollen Grains of Six Taxa of Silenoideae, Caryophyllaceae","authors":"S. Teleb, Rajaa Mabrouk, Marwa M. Eldemerdash","doi":"10.5455/egyjebb.20230131123212","DOIUrl":"https://doi.org/10.5455/egyjebb.20230131123212","url":null,"abstract":"The morphological structures of pollens grains of six taxa belonging to 3 genera of Silenoideae, Caryophyllaceae were examined with the light and scanning electron microscope . In order to elucidate its significance in taxonomy of the group, qualitative and quantitative variables of pollen characters related to the shape, size, aperture characteristics of pores, exine characteristics include exine thickness columellae pattern (LM), tectum (LM) and Sculpture of pollen grain were studied. The pollen grains in the studied species were apolar, polypantoporate, with 12-36 pores. The dominant character of pollen ornamentation is spinulose with variably sparse spinules. Pollen size also varies among different species, and length of the grain diameter are in medium-sized (23-60μm). Cluster and principal component analysis (PCA) of qualitative and quantitative data were used to demonstrate the pollen grains similarities among the species. UPGMA tree derived from cluster analysis reveals two major clusters, the first of which consists of three species (Silene dioca, Silene vulgaris subsp. thorei and Silene pusilla. The second cluster comprises the rest of species Silene vulgaris subsp. bosniaca, Dianthus gratianopolitanus and Minuartia mutabilis, a more pollen grains description to be obtained results in comparative manner to these taxa which belong the family.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80963652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5455/egyjebb.20230325111446
S. Sabbagh, Shimaa S. Hussien, H. Mira, O. Desouky, Dina M. Elgohary
Fungi and their derivatives such as chitosan able to influence the content of ores highlight the complex and dynamic nature of mineral biogeochemistry. Understanding the role of fungi in ore extraction can help to develop more sustainable and efficient mining practices in the future. Chitosan is a biopolymer is naturally originated product that can be derived from the exoskeleton of crustaceans and insects, respectively, and can also be obtained from fungal sources. Chitosan is a versatile hydrophilic polysaccharide derived from chitin. The extraction and characterization of this biopolymer from fungal isolates involves a series of chemical and physical processes to purify and modify the material. The study of the productive applications of chitosan encompasses a wide range of fields, including agriculture, medicine, and environmental management. This biopolymer has been found to have various properties such as biocompatibility, biodegradability, and antimicrobial activity, making it useful in various industries and applications. The extraction and characterization of chitosan from fungal sources and the study of their productive applications is an ongoing area of research with the potential for significant technological advancements and contributions to sustainability. Finally, applications of chitosan-based biomaterials, such as antifungal effects of were estimated by plate well diffusion method against Aspergillus ficuum, Aspergillus fumigatus and Fusarium oxysporum.
{"title":"Enhancement study for production, extraction, and characterization of fungal species chitosan","authors":"S. Sabbagh, Shimaa S. Hussien, H. Mira, O. Desouky, Dina M. Elgohary","doi":"10.5455/egyjebb.20230325111446","DOIUrl":"https://doi.org/10.5455/egyjebb.20230325111446","url":null,"abstract":"Fungi and their derivatives such as chitosan able to influence the content of ores highlight the complex and dynamic nature of mineral biogeochemistry. Understanding the role of fungi in ore extraction can help to develop more sustainable and efficient mining practices in the future. Chitosan is a biopolymer is naturally originated product that can be derived from the exoskeleton of crustaceans and insects, respectively, and can also be obtained from fungal sources. Chitosan is a versatile hydrophilic polysaccharide derived from chitin. The extraction and characterization of this biopolymer from fungal isolates involves a series of chemical and physical processes to purify and modify the material. The study of the productive applications of chitosan encompasses a wide range of fields, including agriculture, medicine, and environmental management. This biopolymer has been found to have various properties such as biocompatibility, biodegradability, and antimicrobial activity, making it useful in various industries and applications. The extraction and characterization of chitosan from fungal sources and the study of their productive applications is an ongoing area of research with the potential for significant technological advancements and contributions to sustainability. Finally, applications of chitosan-based biomaterials, such as antifungal effects of were estimated by plate well diffusion method against Aspergillus ficuum, Aspergillus fumigatus and Fusarium oxysporum.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75271344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5455/egyjebb.20220129014020
Magda Elaraby, H. Ahmad, Nayira Abbas
Echinops spinosus and Fagonia mollis were two plants collected from Wadi Hagul, Eastern desert, Egypt during spring and autumn season. Secondary metabolites were at their highest levels in both tested plants during autumn season, and the antioxidant capabilities of these secondary metabolites were physiologically relevant to plant stress response systems. The invitro studies revealed that both plants have antioxidant properties and a promising cytotoxic activity against a breast cancer cell line (MCF-7). Based on the least of IC50 value of this extract and fractions, GC/ MS analysis was done for E. spinosus and F. mollis methylene chloride fractions and F. mollis crude extract. The GC/MS analysis disclosed that both E. spinosus and F. mollis methylene chloride fractions and their crude extracts, have predominant phytochemical compounds that have cytotoxic activity. The presence of pentanoic acid, 4-oxo-, butyl ester in E. spinosus methylene chloride fraction, and mome inositol and 9,12,15-octadecatrienoic acid, methyl ester components in F. mollis crude extract as well as phytol and (-)-loliolide in its methylene chloride fraction.
{"title":"On The Antioxidant Activity and Cytotoxicity of Echinops spinosus and Fagonia mollis in Wadi Hagul, Egypt","authors":"Magda Elaraby, H. Ahmad, Nayira Abbas","doi":"10.5455/egyjebb.20220129014020","DOIUrl":"https://doi.org/10.5455/egyjebb.20220129014020","url":null,"abstract":"Echinops spinosus and Fagonia mollis were two plants collected from Wadi Hagul, Eastern desert, Egypt during spring and autumn season. Secondary metabolites were at their highest levels in both tested plants during autumn season, and the antioxidant capabilities of these secondary metabolites were physiologically relevant to plant stress response systems. The invitro studies revealed that both plants have antioxidant properties and a promising cytotoxic activity against a breast cancer cell line (MCF-7). Based on the least of IC50 value of this extract and fractions, GC/ MS analysis was done for E. spinosus and F. mollis methylene chloride fractions and F. mollis crude extract. The GC/MS analysis disclosed that both E. spinosus and F. mollis methylene chloride fractions and their crude extracts, have predominant phytochemical compounds that have cytotoxic activity. The presence of pentanoic acid, 4-oxo-, butyl ester in E. spinosus methylene chloride fraction, and mome inositol and 9,12,15-octadecatrienoic acid, methyl ester components in F. mollis crude extract as well as phytol and (-)-loliolide in its methylene chloride fraction.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76554741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5455/egyjebb.20230607015627
Shimaa Sapagh, Mohamed Monem, Esraa A. A. Badr, M. Yassine, N. Elsayed, A. Shala
Staphylococcus aureus is a major foodborne pathogen in raw and ready-to-eat meat products. In this study, the effectiveness of methanolic extracts of Artemisia herbal-alba in inhibiting pan-resistant and enterotoxic Staphylococcus aureus was investigated. The first analysis showed a high prevalence (33%) of coagulase-positive staphylococci in imported meat samples from retail stores. Examination of antibiotic resistance patterns revealed that staphylococcal antibiogram resistance profiles were diverse: five strains demonstrated resistance to at least one antimicrobial agent, fourteen isolates demonstrated multidrug resistance (MDR) and one resistant to all test antibiotics (PAN). Multiplex PCR of pan-Staphylococcus strains revealed positive serotypes for the enterotoxin genes seb, seg and sei at 665.05 bp, 277.99 bp and 460.74 bp, respectively, and a positive serotype for the mec-A gene at 538.45 bp. Compared to the tetracycline effect, methanolic extracts of Artemisia herba-alba showed significant inhibitory effects against pan-resistant Staphylococcus aureus. Real-time quantitative PCR data analysis showed that Artemisia herba-alba methanolic extracts caused a significant decrease in the expression levels of the genes mecA, mecC, sei, seg and seb. Therefore, the methanolic extract of Artemisia herba-alba has the potential to be a promising natural product to control foodborne pathogens and could be valuable for food safety applications.
{"title":"Molecular characterization of enterotoxin and antibiotic resistance genes-producing Staphylococcus aureus derived from imported meat: Artemisia herb‐alba extract as an antibacterial agent","authors":"Shimaa Sapagh, Mohamed Monem, Esraa A. A. Badr, M. Yassine, N. Elsayed, A. Shala","doi":"10.5455/egyjebb.20230607015627","DOIUrl":"https://doi.org/10.5455/egyjebb.20230607015627","url":null,"abstract":"Staphylococcus aureus is a major foodborne pathogen in raw and ready-to-eat meat products. In this study, the effectiveness of methanolic extracts of Artemisia herbal-alba in inhibiting pan-resistant and enterotoxic Staphylococcus aureus was investigated. The first analysis showed a high prevalence (33%) of coagulase-positive staphylococci in imported meat samples from retail stores. Examination of antibiotic resistance patterns revealed that staphylococcal antibiogram resistance profiles were diverse: five strains demonstrated resistance to at least one antimicrobial agent, fourteen isolates demonstrated multidrug resistance (MDR) and one resistant to all test antibiotics (PAN). Multiplex PCR of pan-Staphylococcus strains revealed positive serotypes for the enterotoxin genes seb, seg and sei at 665.05 bp, 277.99 bp and 460.74 bp, respectively, and a positive serotype for the mec-A gene at 538.45 bp. Compared to the tetracycline effect, methanolic extracts of Artemisia herba-alba showed significant inhibitory effects against pan-resistant Staphylococcus aureus. Real-time quantitative PCR data analysis showed that Artemisia herba-alba methanolic extracts caused a significant decrease in the expression levels of the genes mecA, mecC, sei, seg and seb. Therefore, the methanolic extract of Artemisia herba-alba has the potential to be a promising natural product to control foodborne pathogens and could be valuable for food safety applications.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82727693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5455/egyjebb.20230209083158
A. Shehata, W. Taia, M. Ibrahim, I. Shamy
The morphology and morphometric of stems, leaves, inflorescence, flowers and fruits of 18 species of Plantago L. (Plantaginaceae sensu stricto (s.s.) as well as 18 related taxa that were formerly assigned to the Scrophulariaceae , representing the genera : Anarrhinum, Antirrhinum, Digitalis ,Linaria, Kickxia, Russelia, Verbascum and, Veronica were investigated . To clarify the taxonomic relationships among all studied taxa and provide more information about the phenetic relationships of the investigated species of Plantago and its infrageneric classification ,107 morphological characters were numerically analyzed using the UPGMA clustering method ∙The resulting phenogram clearly showed the separation of the Plantago species from the investigated scrophulariaceous species∙ This result supports the earlier taxonomic views in the isolation of Plantago in its own mono-generic family (Plantaginaceae) apart from other Scrophulariaceous species ∙Additionally, the study showed that Veronica possessed certain affinities to Plantago species, thus giving extra support to the earlier views that Plantaginaceae sensu stricto and Scrophulariaceae sensu lato are allied through Veronica ∙Moreover , the obtained data provided some evidence for the infrageneric classification of Plantago and corresponded with the ,classification systems proposed by Rhan (1978) and Shipunov(2021)∙
对车前草属(Plantaginaceae sensu stricto, s.s.) 18种车前草属(Anarrhinum, Antirrhinum, Digitalis,Linaria, Kickxia, Russelia, Verbascum, Veronica)的茎、叶、花序、花和果实的形态特征进行了研究。为明确各研究类群间的分类学关系，提供更多关于所调查车前草种间的亲缘关系及其属内分类的信息，采用UPGMA聚类方法对107个形态学特征进行了数值分析，所得的物候图清晰地显示了车前草种与所调查的伞属植物的分离∙这一结果支持了早前关于车前草分离的分类学观点此外，研究表明，Veronica与车前草属植物具有一定的亲缘关系，从而进一步支持了早期关于Plantaginaceae sensu stricto和Scrophulariaceae sensu lato通过Veronica∙亲缘关系的观点。所得数据与Rhan(1978)和Shipunov(2021)∙提出的分类体系一致，为车前草的近属分类提供了一定的依据
{"title":"Taxonomic study of (Plantaginaceae Juss.) and allied genera of Scrophulariaceae Juss∙","authors":"A. Shehata, W. Taia, M. Ibrahim, I. Shamy","doi":"10.5455/egyjebb.20230209083158","DOIUrl":"https://doi.org/10.5455/egyjebb.20230209083158","url":null,"abstract":"The morphology and morphometric of stems, leaves, inflorescence, flowers and fruits of 18 species of Plantago L. (Plantaginaceae sensu stricto (s.s.) as well as 18 related taxa that were formerly assigned to the Scrophulariaceae , representing the genera : Anarrhinum, Antirrhinum, Digitalis ,Linaria, Kickxia, Russelia, Verbascum and, Veronica were investigated . To clarify the taxonomic relationships among all studied taxa and provide more information about the phenetic relationships of the investigated species of Plantago and its infrageneric classification ,107 morphological characters were numerically analyzed using the UPGMA clustering method ∙The resulting phenogram clearly showed the separation of the Plantago species from the investigated scrophulariaceous species∙ This result supports the earlier taxonomic views in the isolation of Plantago in its own mono-generic family (Plantaginaceae) apart from other Scrophulariaceous species ∙Additionally, the study showed that Veronica possessed certain affinities to Plantago species, thus giving extra support to the earlier views that Plantaginaceae sensu stricto and Scrophulariaceae sensu lato are allied through Veronica ∙Moreover , the obtained data provided some evidence for the infrageneric classification of Plantago and corresponded with the ,classification systems proposed by Rhan (1978) and Shipunov(2021)∙","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90259844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5455/egyjebb.20230709064439
Mohamed Aldamanhory, K. Allah, S. Shabana, Maha M. Azab
In recent years, it has been evident that there is a rise in global interest in the discovery of drugs from medicinal plants. The present study aimed to estimate the antibacterial and antioxidant activities of ethanol extract of five plant species: Lavandula atriplicifolia, Cocculus pendulus, Teucrium polium, Varthemia candicans, and Cassia javanica against multidrug-resistant bacterial isolates. In the present study, 107 different clinical specimens were collected and cultured resulting in seventy-nine bacterial isolates. The antibiotic-resistant pattern revealed the presence of seventeen multidrug-resistant bacteria. In vitro, antibacterial activity was evaluated against the seventeen resistant bacterial isolates by agar well diffusion method. Among the plants screened, the ethanol extracts of V. candicans and C. javanica showed the best antibacterial activity. The highest total phenol content was shown by L. atriplicifolia, Cassia javanica, and V. candicans ethanol extracts, while the highest flavonoid content was shown by L. atriplicifolia and T. polium extracts. The ethanol extracts of T. polium, L. atriplicifolia, V. candicans, and C. pendulus recorded significant DPPH scavenging activity, while the extracts of C. javanica, V. candicans, and T. polium recorded high PMA values. The results indicated that the investigated plants possessed significant antibacterial and antioxidant properties and could be a vital source of natural compounds for the development of novel drugs.
{"title":"Exploring the antibacterial and antioxidant potentials of some Egyptian wild plant species.","authors":"Mohamed Aldamanhory, K. Allah, S. Shabana, Maha M. Azab","doi":"10.5455/egyjebb.20230709064439","DOIUrl":"https://doi.org/10.5455/egyjebb.20230709064439","url":null,"abstract":"In recent years, it has been evident that there is a rise in global interest in the discovery of drugs from medicinal plants. The present study aimed to estimate the antibacterial and antioxidant activities of ethanol extract of five plant species: Lavandula atriplicifolia, Cocculus pendulus, Teucrium polium, Varthemia candicans, and Cassia javanica against multidrug-resistant bacterial isolates. In the present study, 107 different clinical specimens were collected and cultured resulting in seventy-nine bacterial isolates. The antibiotic-resistant pattern revealed the presence of seventeen multidrug-resistant bacteria. In vitro, antibacterial activity was evaluated against the seventeen resistant bacterial isolates by agar well diffusion method. Among the plants screened, the ethanol extracts of V. candicans and C. javanica showed the best antibacterial activity. The highest total phenol content was shown by L. atriplicifolia, Cassia javanica, and V. candicans ethanol extracts, while the highest flavonoid content was shown by L. atriplicifolia and T. polium extracts. The ethanol extracts of T. polium, L. atriplicifolia, V. candicans, and C. pendulus recorded significant DPPH scavenging activity, while the extracts of C. javanica, V. candicans, and T. polium recorded high PMA values. The results indicated that the investigated plants possessed significant antibacterial and antioxidant properties and could be a vital source of natural compounds for the development of novel drugs.","PeriodicalId":22404,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)","volume":"118 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88060571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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