Addition of Glutamine in the Human Platelets Could Prevent Toxic Effect of Ultraviolet-C Induced Platelets Activation

Abstract

The primary function of platelets is to prevent bleeding. Transfusion of platelets can be prophylactic or therapeutic. From the storage perspective of blood and blood components, standard protocols are followed around the world to ensure the safe operation of blood banks. The Use of UV-C light in treating platelets has become a valuable method for storage and efficacy of platelets concentration in the blood bank. However, its deleterious effect remains, such as activation of platelets, thus losing their physiological function. In this study we intend to demonstrate that addition of glutamine in the platelets concentrate could prevent the toxic effect of UV-C. This study was conducted using human or mouse platelets. Use of positive and negative control in all experiment were ensured to validate the findings. In vitro thrombus formations was assessed before and after exposure of platelets to appropriate dose of UV-C. To assess the cytotoxic effect of 50 mM of glutamine on the platelets, methylthiazoletetrazolium (MTT) was used to validate the viability effect. We have successfully demonstrated that physiological damages done during the exposure of platelet concentrate to UV-C could be alleviated by the use of glutamine. Our study demonstrated that the toxic effect of UV-C on the platelets could be preserved by adding appropriate concentration of glutamine.