The serology of Pseudomonas aeruginosa analysed by means of quantitative immunoelectrophoretic methods. III. Reproducibility of a polyvalent P. aeruginosa reference standard-antigen.

Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 2009-08-15 DOI:10.1111/J.1699-0463.1975.TB00122.X

N. Høiby

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引用次数: 18

Abstract

The reproducibility of a polyvalent Pseudomonas aeruginosa antigen (St-Ag) composed of a mixture of antigens from 4 O groups of this bacterium has been studied. Ten batches of St-Ag were produced, and each of these and each of the 10 batches of antigens from the 4 strains of P. aeruginosa were compared with St-Ag batch 1 by means of quantitative immunoelectrophoretic methods and a polyvalent antiserum (St-Ab) raised against St-Ag. Fifty-three of the 55 antigens of St-Ag were stable and could be reproduced with reasonable precision in all 10 batches, and 3 of the 4 strains of P. aeruginosa were stable in antigen composition in all batches. One of the strains (0-5A) had lost 2 antigens in the last 5 batches, and the concentrations of 7 other antigens were simultaneously changed, reflecting a smooth-rough dissociation. The disappearance of the 2 antigens in the latest 5 batches of 0-5A was also reflected in similar changes in the antigen composition of the latest 5 batches of St-Ag.

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采用定量免疫电泳方法对铜绿假单胞菌进行血清学分析。3多价铜绿假单胞菌参比标准抗原的可重复性。

研究了铜绿假单胞菌多价抗原(St-Ag)的可重复性，该抗原由该细菌的40个抗原群的混合物组成。制备了10批St-Ag，并采用定量免疫电泳法和St-Ag多价抗血清(St-Ab)对4株铜绿假单胞菌的10批抗原进行了比较。55株St-Ag抗原中53株在10批中均能稳定重现，且重现精度合理;4株铜绿假单胞菌中3株抗原组成在10批中均稳定。其中1株(0-5A)在最后5批中丢失了2个抗原，其他7个抗原的浓度同时发生变化，反映了一个光滑粗糙的解离过程。最近5批0-5A中2种抗原的消失也反映在最近5批St-Ag抗原组成的类似变化上。

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Acta pathologica et microbiologica Scandinavica. Section B, Microbiology

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