Pub Date : 2009-08-19DOI: 10.1111/J.1699-0463.1981.TB00146_89B.X
L. Rönsholt
Various auxiliary treatments of L-cells employed for the isolation and cultivation of C. psittaci were investigated in order to develop an improved method for the detection of the agent, in addition to the aid obtained by centrifugation and cycloheximide treatment. Glucocorticoid treatment increased the observed number of inclusions considerably through a preservative effect on host cells and enhanced an spontaneous re-infection. Besides, the hormone made the scanning of cell culture for inclusions more convenient through an altered cell morphology. This method was tested with two extreme species types that differed as regards cytopathogenicity and growth rate. The length of the cultivation period was of great importance for the diagnostic result. Especially the cytopathogenic agent-type influenced the optimal time of cell culture fixation, which was situated around 48 or 88 hours post infection (h p.i.). Owing to the cytotoxicity of field samples (milk secretion), the cell culture technique (48 h p.i.) was less sensitive compared to the conventional isolation method in embryonated eggs. However, a different sampling technique improved the result, and simultaneous use of the secondary multiplication cycle of chlamydia (88 h p.i.) makes the less cumbersome cell culture technic recommendable.
研究了除离心和环己亚胺处理外，用于鹦鹉螺分离和培养的l细胞的各种辅助处理，以建立一种改进的试剂检测方法。糖皮质激素处理通过对宿主细胞的保存作用显著增加了观察到的包涵体数量，并增强了自发再感染。此外，这种激素通过改变细胞形态，使细胞培养物的内含物扫描更方便。该方法在细胞致病性和生长速度不同的两种极端物种类型中进行了试验。培养时间的长短对诊断结果有重要影响。细胞致病菌类型影响细胞培养固定的最佳时间，在感染后48或88小时左右(h p.i.)。由于现场样品的细胞毒性(乳汁分泌)，细胞培养技术(48 h p.i)与传统的胚胎卵分离方法相比敏感度较低。然而，一种不同的取样技术改善了结果，同时使用衣原体的二次增殖周期(88 h p.i)使不那么麻烦的细胞培养技术得到推荐。
{"title":"A modified isolation technique for Chlamydia psittaci in L-cells treated with cycloheximide and glucocorticoid.","authors":"L. Rönsholt","doi":"10.1111/J.1699-0463.1981.TB00146_89B.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1981.TB00146_89B.X","url":null,"abstract":"Various auxiliary treatments of L-cells employed for the isolation and cultivation of C. psittaci were investigated in order to develop an improved method for the detection of the agent, in addition to the aid obtained by centrifugation and cycloheximide treatment. Glucocorticoid treatment increased the observed number of inclusions considerably through a preservative effect on host cells and enhanced an spontaneous re-infection. Besides, the hormone made the scanning of cell culture for inclusions more convenient through an altered cell morphology. This method was tested with two extreme species types that differed as regards cytopathogenicity and growth rate. The length of the cultivation period was of great importance for the diagnostic result. Especially the cytopathogenic agent-type influenced the optimal time of cell culture fixation, which was situated around 48 or 88 hours post infection (h p.i.). Owing to the cytotoxicity of field samples (milk secretion), the cell culture technique (48 h p.i.) was less sensitive compared to the conventional isolation method in embryonated eggs. However, a different sampling technique improved the result, and simultaneous use of the secondary multiplication cycle of chlamydia (88 h p.i.) makes the less cumbersome cell culture technic recommendable.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"22 1","pages":"13-23"},"PeriodicalIF":0.0,"publicationDate":"2009-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77819645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-19DOI: 10.1111/J.1699-0463.1981.TB00156_89B.X
P. Gaustad, J. Eriksen
Two new media were developed, containing only the dialyzable components of Todd Hewitt broth (TH) with (medium II) or without (medium IV) inactivated horse serum. The two media were used to detect activity of the competence factor (CF) and the competence factor inactivator (CGI) of Streptococcus sanguis, and in preliminary experiments of CF isolation. Because all the dissolved substances with molecular weight (mol. wt.) less than 12,000 can be removed from these media by dialysis, leaving the CF and possible other high mol. wt. substances formed by growth in the dialysis tube, the use of these media should facilitate the isolation of CF and other high mol. wt. substances involved in genetic transformation of S. sanguis. Dialysis experiments suggest a mol. wt. greater than 12,000 of the CFs of the strains Challis and 13b. The CF of strain 13b was further isolated by Sephadex gel filtration and was eluted in the fractions of low mol. wt. compounds.
{"title":"Genetic transformation of Streptococcus sanguis. Further studies on the production and isolation of the competence factor.","authors":"P. Gaustad, J. Eriksen","doi":"10.1111/J.1699-0463.1981.TB00156_89B.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1981.TB00156_89B.X","url":null,"abstract":"Two new media were developed, containing only the dialyzable components of Todd Hewitt broth (TH) with (medium II) or without (medium IV) inactivated horse serum. The two media were used to detect activity of the competence factor (CF) and the competence factor inactivator (CGI) of Streptococcus sanguis, and in preliminary experiments of CF isolation. Because all the dissolved substances with molecular weight (mol. wt.) less than 12,000 can be removed from these media by dialysis, leaving the CF and possible other high mol. wt. substances formed by growth in the dialysis tube, the use of these media should facilitate the isolation of CF and other high mol. wt. substances involved in genetic transformation of S. sanguis. Dialysis experiments suggest a mol. wt. greater than 12,000 of the CFs of the strains Challis and 13b. The CF of strain 13b was further isolated by Sephadex gel filtration and was eluted in the fractions of low mol. wt. compounds.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"96 1","pages":"75-80"},"PeriodicalIF":0.0,"publicationDate":"2009-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83991338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-19DOI: 10.1111/J.1699-0463.1981.TB00145_89B.X
Birger R. Møller
Four serological tests, viz. indirect hemagglutination (IHA), metabolism inhibition (MI), immunofluorescence (IMF), and a modification of the growth-inhibition (GI) test have been compared for the detection of antibodies against Mycoplasma hominis in female grivet monkeys with experimentally induced pelvic inflammatory disease. Moreover, cold hemagglutinins (CHA), and immunoglobulins, M, G, and A have been determined. The IHA test was found to be superior to the other methods used. The antibodies were present in all inoculated monkeys two weeks after the inoculation and a maximum in titre occurred one week later. Antibodies detected by the modified GI test occurred in all monkeys inoculated with the organism together with the IHA antibodies, but the maximum in titre was less marked. The IMF test was less sensitive than the GI test; the antibodies generally occurred later and disappeared faster. Using the MI test, no antibodies could be detected in any of the monkeys during the experimental period and no CHA antibodies were found in any of the sera. IgM rose earlier and declined more rapidly than IgG. It is concluded that the IHA test is most suitable for the measurement of serum antibodies caused by infection with M. hominis in grivet monkeys. The modified GI test, although less sensitive, may also be useful because of its simplicity in performance.
{"title":"Comparison of serological tests for detection of Mycoplasma hominis antibodies in female Grivet monkeys with experimentally induced salpingitis.","authors":"Birger R. Møller","doi":"10.1111/J.1699-0463.1981.TB00145_89B.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1981.TB00145_89B.X","url":null,"abstract":"Four serological tests, viz. indirect hemagglutination (IHA), metabolism inhibition (MI), immunofluorescence (IMF), and a modification of the growth-inhibition (GI) test have been compared for the detection of antibodies against Mycoplasma hominis in female grivet monkeys with experimentally induced pelvic inflammatory disease. Moreover, cold hemagglutinins (CHA), and immunoglobulins, M, G, and A have been determined. The IHA test was found to be superior to the other methods used. The antibodies were present in all inoculated monkeys two weeks after the inoculation and a maximum in titre occurred one week later. Antibodies detected by the modified GI test occurred in all monkeys inoculated with the organism together with the IHA antibodies, but the maximum in titre was less marked. The IMF test was less sensitive than the GI test; the antibodies generally occurred later and disappeared faster. Using the MI test, no antibodies could be detected in any of the monkeys during the experimental period and no CHA antibodies were found in any of the sera. IgM rose earlier and declined more rapidly than IgG. It is concluded that the IHA test is most suitable for the measurement of serum antibodies caused by infection with M. hominis in grivet monkeys. The modified GI test, although less sensitive, may also be useful because of its simplicity in performance.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"64 1","pages":"7-11"},"PeriodicalIF":0.0,"publicationDate":"2009-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90377742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-19DOI: 10.1111/J.1699-0463.1981.TB00173_89B.X
I. Ørstavik
LLC-MK2, GMK AH-1, BSC-1, and Vero cells were compared in titrations of recent isolates and laboratory strains of influenza A and B and parainfluenza types 1, 2, and 3 viruses. About the same titres, as determined by haemadsorption in cell cultures, were obtained in LLC-MK2, GMK AH-1, and BSC-1 cells when trypsin had been added to the medium, whereas the Vero cells were less sensitive to the influenza virus strains tested. Virus titres were usually low in the absence of trypsin. A laboratory strain of parainfluenza 2 virus reached about the same titres in medium without as in medium with trypsin, possibly owing to prior adaptation by passages in Vero cells. Comparative titrations of influenza A, and parainfluenza 1 and 3 viruses suggested the same susceptibility of LLC-MK2 cells with trypsin as of primary monkey kidney cells. Re-isolation experiments from 38 clinical specimens showed LLC-MK2 cells to be as efficient as primary monkey kidney cells for isolation of influenza and parainfluenza viruses, whereas the susceptibility of the other cell lines to clinical material has not yet been tested on a larger scale. It is concluded that a continuous line of monkey kidney cell culture may be acceptable as an alternative to primary monkey kidney cells for the isolation of influenza and parainfluenza viruses from patients.
{"title":"Susceptibility of continuous lines of monkey kidney cells to influenza and parainfluenza viruses in the presence of trypsin.","authors":"I. Ørstavik","doi":"10.1111/J.1699-0463.1981.TB00173_89B.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1981.TB00173_89B.X","url":null,"abstract":"LLC-MK2, GMK AH-1, BSC-1, and Vero cells were compared in titrations of recent isolates and laboratory strains of influenza A and B and parainfluenza types 1, 2, and 3 viruses. About the same titres, as determined by haemadsorption in cell cultures, were obtained in LLC-MK2, GMK AH-1, and BSC-1 cells when trypsin had been added to the medium, whereas the Vero cells were less sensitive to the influenza virus strains tested. Virus titres were usually low in the absence of trypsin. A laboratory strain of parainfluenza 2 virus reached about the same titres in medium without as in medium with trypsin, possibly owing to prior adaptation by passages in Vero cells. Comparative titrations of influenza A, and parainfluenza 1 and 3 viruses suggested the same susceptibility of LLC-MK2 cells with trypsin as of primary monkey kidney cells. Re-isolation experiments from 38 clinical specimens showed LLC-MK2 cells to be as efficient as primary monkey kidney cells for isolation of influenza and parainfluenza viruses, whereas the susceptibility of the other cell lines to clinical material has not yet been tested on a larger scale. It is concluded that a continuous line of monkey kidney cell culture may be acceptable as an alternative to primary monkey kidney cells for the isolation of influenza and parainfluenza viruses from patients.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"79 1","pages":"179-183"},"PeriodicalIF":0.0,"publicationDate":"2009-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78693580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-19DOI: 10.1111/J.1699-0463.1981.TB00190_89B.X
Gabriel Ånestad, Osvald Ragnvald Mœhle
A preliminary report on a simplified procedure for the preparation of nasopharyngeal suction specimens making direct smears of aspirated material is presented. The smears were examined by the indirect immunofluorescence (IF) technique for the presence of respiratory syncytial (RS) virus antigens. Specimens form 99 children with acute respiratory tract illnesses were collected during the winter season 1978-79, and RS virus was identified in samples from 45 of these patients. Serological investigations run in parallel showed fairly good correlation with the IF examinations of the smears. This simplified procedure for the preparation of nasopharyngeal suction specimens may be recommended when the conventional preparation cannot readily be performed.
{"title":"Rapid diagnosis of respiratory syncytial (RS) virus infection by immunofluorescence: a simplified procedure for the preparation of nasopharyngeal suction specimens.","authors":"Gabriel Ånestad, Osvald Ragnvald Mœhle","doi":"10.1111/J.1699-0463.1981.TB00190_89B.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1981.TB00190_89B.X","url":null,"abstract":"A preliminary report on a simplified procedure for the preparation of nasopharyngeal suction specimens making direct smears of aspirated material is presented. The smears were examined by the indirect immunofluorescence (IF) technique for the presence of respiratory syncytial (RS) virus antigens. Specimens form 99 children with acute respiratory tract illnesses were collected during the winter season 1978-79, and RS virus was identified in samples from 45 of these patients. Serological investigations run in parallel showed fairly good correlation with the IF examinations of the smears. This simplified procedure for the preparation of nasopharyngeal suction specimens may be recommended when the conventional preparation cannot readily be performed.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"55 1","pages":"285-287"},"PeriodicalIF":0.0,"publicationDate":"2009-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84749896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1976.TB01965.X
C. Örvell
Egg-grown Sendai virus was used for preparation of rabbit hyperimmune sera directed against purified whole virus and pronasetreated projectionless virus particles. These sera and convalescent sera after natural Sendai infection in guinea pigs were studied in haemolysis-inhibition (HLI), haemagglutination-inhibition (HI) and neuraminidase-inhibition (NI) tests both before and after absorption with Tween 80-ether (TE) treated virus preparations. In addition, neutralization tests using the different sera were carried out. HI and NI antibodies and the major population of neutralizing antibodies in convalescent sera were removed by absorption with TE treated virus material without changing the titre of non-HI HLI antibodies. Rabbit hyperimmune sera directed against projectionless virus particles exhibited HLI antibody titres in marked excess of HI and NI antibody titres, whereas this was not found in sera against purified whole virus. In contrast, absorption of sera against projectionless particles eliminated HI antibodies without changing the titre of non-HI HLI antibodies. The protein composition of antigenic preparations used in absorption experiments and for preparation of sera was investigated by SDS-polyacryladmie-gel electrophoresis. TH treatment had no significant effect on the polypeptide pattern of Sendai virus. Pronase-treatment predominantly affected the two glycosylated proteins of Sendai virus. The larger glycoprotein was not detectable in pronasetreated projectionless virus particles, whereas the smaller glycoprotein was present in reduced quantities.
{"title":"Identification of paramyxovirus-specific haemolysis-inhibiting antibodies separate from haemagglutinating-inhibiting and neuraminidase-inhibiting antibodies. 1. Sendai virus haemolysis-inhibiting antibodies.","authors":"C. Örvell","doi":"10.1111/J.1699-0463.1976.TB01965.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1976.TB01965.X","url":null,"abstract":"Egg-grown Sendai virus was used for preparation of rabbit hyperimmune sera directed against purified whole virus and pronasetreated projectionless virus particles. These sera and convalescent sera after natural Sendai infection in guinea pigs were studied in haemolysis-inhibition (HLI), haemagglutination-inhibition (HI) and neuraminidase-inhibition (NI) tests both before and after absorption with Tween 80-ether (TE) treated virus preparations. In addition, neutralization tests using the different sera were carried out. HI and NI antibodies and the major population of neutralizing antibodies in convalescent sera were removed by absorption with TE treated virus material without changing the titre of non-HI HLI antibodies. Rabbit hyperimmune sera directed against projectionless virus particles exhibited HLI antibody titres in marked excess of HI and NI antibody titres, whereas this was not found in sera against purified whole virus. In contrast, absorption of sera against projectionless particles eliminated HI antibodies without changing the titre of non-HI HLI antibodies. The protein composition of antigenic preparations used in absorption experiments and for preparation of sera was investigated by SDS-polyacryladmie-gel electrophoresis. TH treatment had no significant effect on the polypeptide pattern of Sendai virus. Pronase-treatment predominantly affected the two glycosylated proteins of Sendai virus. The larger glycoprotein was not detectable in pronasetreated projectionless virus particles, whereas the smaller glycoprotein was present in reduced quantities.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"89 1","pages":"441-450"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80917513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/j.1699-0463.1976.tb01894.x
E. Holten
The catabolism of pyruvate and acetate is selected Neisseria species was studied by radiorespirometry. Both substrates were oxidized via the tricarboxylic acid cycle. N. elongata and the "false neisserias" (N. catarrhalis, N. ovis and N. caviae) did oxidize acetate in the absence of other substrates. This can be explained if it is assumed that these species have glyoxylic acid cycle activity. In the "true neisserias" other than N. elongata, acetate was oxidized only in the presence of glutamate, indicating that these species do not possess a glyoxylic acid cycle.
{"title":"Radiorespirometric studies in genus Neisseria. 3. The catabolism of pyruvate and acetate.","authors":"E. Holten","doi":"10.1111/j.1699-0463.1976.tb01894.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1976.tb01894.x","url":null,"abstract":"The catabolism of pyruvate and acetate is selected Neisseria species was studied by radiorespirometry. Both substrates were oxidized via the tricarboxylic acid cycle. N. elongata and the \"false neisserias\" (N. catarrhalis, N. ovis and N. caviae) did oxidize acetate in the absence of other substrates. This can be explained if it is assumed that these species have glyoxylic acid cycle activity. In the \"true neisserias\" other than N. elongata, acetate was oxidized only in the presence of glutamate, indicating that these species do not possess a glyoxylic acid cycle.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"34 1","pages":"9-16"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87025544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1976.TB01947.X
I. Ørskov, F. Ørskov
: Five Escherichia coli strains were established as antigenic test strains for five new polysaccharide K antigens: K95, K96, K97, K98 and K100. K95 to K98 served already as test strains of O antigens O75, O77, O81 and O107 respectively. F147, which is test strain of K100, had O antigen O75.
{"title":"FIVE NEW ESCHERICHIA COLI K ANTIGENS, K95, K96, K97, K98 AND K100","authors":"I. Ørskov, F. Ørskov","doi":"10.1111/J.1699-0463.1976.TB01947.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1976.TB01947.X","url":null,"abstract":": Five Escherichia coli strains were established as antigenic test strains for five new polysaccharide K antigens: K95, K96, K97, K98 and K100. K95 to K98 served already as test strains of O antigens O75, O77, O81 and O107 respectively. F147, which is test strain of K100, had O antigen O75.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"9 1","pages":"321-325"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87771377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1975.TB00109.X
N. Høiby
The occurrence of antibodies against antigens prepared from strains representing 13 O groups of Pseudomonas aeruginosa and against a polyvalent Ps. aeruginosa antigen (St-Ag) has been investigated in sera from 100 patients. By means of fused rocket immunoelectrophoresis with intermediate gel it was found that the humoral immune response against Ps. aeruginosa resulting in precipitating antibodies will be detected by St-Ag as well as by any other of the antigen samples investigated. Six of the sera contained group-specific antibodies which were revealed by only one of the antigen samples used and not by St-Ag. These six sera were further studied by means of various quantitative immunoelectrophoretic methods using St-Ag as well as antigens prepared from the infecting Ps. aeruginosa strain in the patient concerned. In all six sera, only one extra precipitin could be detected using antigens prepared from the homologous strain instead of St-Ag. This extra precipitin corresponded presumably to group-specific O-antigens not included in St-Ag. In sera from patients, these group-specific antibodies were always accompanied by antibodies against antigens common to all strains of Ps. aeruginosa.
{"title":"The serology of Pseudomonas aeruginosa analysed by means of quantitative immunoelectrophoretic methods. II. Comparison of the antibody response in man against thirteen O groups of Ps. aeruginosa.","authors":"N. Høiby","doi":"10.1111/J.1699-0463.1975.TB00109.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1975.TB00109.X","url":null,"abstract":"The occurrence of antibodies against antigens prepared from strains representing 13 O groups of Pseudomonas aeruginosa and against a polyvalent Ps. aeruginosa antigen (St-Ag) has been investigated in sera from 100 patients. By means of fused rocket immunoelectrophoresis with intermediate gel it was found that the humoral immune response against Ps. aeruginosa resulting in precipitating antibodies will be detected by St-Ag as well as by any other of the antigen samples investigated. Six of the sera contained group-specific antibodies which were revealed by only one of the antigen samples used and not by St-Ag. These six sera were further studied by means of various quantitative immunoelectrophoretic methods using St-Ag as well as antigens prepared from the infecting Ps. aeruginosa strain in the patient concerned. In all six sera, only one extra precipitin could be detected using antigens prepared from the homologous strain instead of St-Ag. This extra precipitin corresponded presumably to group-specific O-antigens not included in St-Ag. In sera from patients, these group-specific antibodies were always accompanied by antibodies against antigens common to all strains of Ps. aeruginosa.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"136 1","pages":"328-34"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77461343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1977.TB01673.X
D. Ørstavik
Macromolecular solutes (albumin, concanavalin A, whole saliva, serum) caused impaired sorption of Streptococcus faecium and Streptococcus sanguis to glass. The inhibitory effect resided chiefly with interactions of the solutes with the glass surface. In the case of sorption of S. sanguis to glass in the presence of parotid fluid, the inhibitory effect was counteracted by a specific attachment of S. sanguis cells mediated by some component(s) of the parotid fluid. Agglutination of the test organisms was in general accompanied by inhibition of sorption. However, when small or unstable aggregates were formed, the number of cells adhering on the glass surface was increased. The findings are discussed with reference to the colonization of teeth by oral bacteria.
{"title":"Sorption of Streptococci to glass: Effects of macromolecular solutes.","authors":"D. Ørstavik","doi":"10.1111/J.1699-0463.1977.TB01673.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1977.TB01673.X","url":null,"abstract":"Macromolecular solutes (albumin, concanavalin A, whole saliva, serum) caused impaired sorption of Streptococcus faecium and Streptococcus sanguis to glass. The inhibitory effect resided chiefly with interactions of the solutes with the glass surface. In the case of sorption of S. sanguis to glass in the presence of parotid fluid, the inhibitory effect was counteracted by a specific attachment of S. sanguis cells mediated by some component(s) of the parotid fluid. Agglutination of the test organisms was in general accompanied by inhibition of sorption. However, when small or unstable aggregates were formed, the number of cells adhering on the glass surface was increased. The findings are discussed with reference to the colonization of teeth by oral bacteria.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"46 1","pages":"47-53"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83745686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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