Molecularly imprinted monodisperse microspheres for competitive radioassay

L. Ye, P. Cormack, K. Mosbach
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引用次数: 274

Abstract

In the present study, molecularly imprinted affinity sorbents against theophylline and 17β-estradiol are synthesised via precipitation polymerisation, a synthetic method that yields monodisperse, spherical polymer particles in the micron-scale range, quickly, cleanly and in good yield. The specific binding sites that are created during the imprinting process are analysed via radioligand binding analysis. The molecularly imprinted microspheres are highly specific and have higher load capacities compared to the ‘classical’ particles obtained by grinding the imprinted monolith. The successful imprinting against model compounds with very different hydrophobicities demonstrates the generality of the current simple approach.
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竞争性放射测定用分子印迹单分散微球
在本研究中,通过沉淀聚合法合成了针对茶碱和17β-雌二醇的分子印迹亲和吸附剂,这种合成方法可以在微米尺度范围内产生单分散的球形聚合物颗粒,快速、清洁且收率高。印迹过程中产生的特定结合位点通过放射配基结合分析进行分析。分子印迹微球具有高度特异性,与通过研磨印迹单体获得的“经典”颗粒相比,具有更高的负载能力。对具有不同疏水性的模型化合物的成功印迹表明了当前简单方法的普遍性。
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