Features of the introduction of Berberis iliensis M. Pop. into in vitro culture

Abstract

The results of the introduction of Berberis iliensis M. Pop., a rare endemic plant, into in vitro culture are presented. The rare species is currently endangered. Methods of introduction into in vitro culture have been worked out, sterilizing agents and sterilization modes have been selected (5% bleach solution “Belizna”, treatment time 5 min, 10% hydrogen peroxide solution, exposure time 7 min). The WPM nutrient medium, with a pH level of 5.6–5.8, was selected as optimal for the successful development of Berberis iliensis plants in in vitro culture. Vitamins were added to the standard mineral composition of the medium. Magentas with explants were cultivated in a room without access to light at a temperature of 22–25°C and a humidity of 70% for 3–4 weeks. Further cultivation was carried out at 26°C, a light period of 16 h, and illumination of 4000–5000 lux. Aseptic cultures of B. iliensis were obtained and they had a regenerative capacity. Shoots were formed without callus, that is, direct regeneration is observed, which is the most favorable for micropropagation.