Identification of species of emeria of turkeys using regression analysis of morphometric indicators of ocycists

P. Liulin, M. Bogach, O. Getmanets
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Abstract

The aim. Identify the species of oocysts of turkeys by morphometric parameters. Material and methods. Eimeria oocysts obtained from faeces of suspects and patients with spontaneous eimeriosis of turkeys of poultry farms of Kharkiv region served as material for the research. Methods used: parasitological, coprological, light microscopy, morphometry, mathematical and statistical, correlation and regression analysis, ANOVA variation statistics. Results. Morphometrically (n=255) samples of turkey eimeria oocysts were studied and identified to the species: E. gallopavonis (n=50), E. meleagrimitis (n=50), E. adenoids (n=51), E. meleagridis n=53), E. innocua (n=51) according to identification indicators (X1 - length of the oocyst in μm; X2 - width of the oocyst in μm; X3 - area of the oocyst in μm; X4 - eccentricity of the model ellipse; X5 - ratio of the width of the oocyst to its length; X6 - largest curvature and X7 - smallest curvature in its model ellipse poles on the major and minor axes, respectively, in μm, X8 - presence - 1 or absence - 0 polar granules) which are mathematical expressions of morphometric dependences of the structure of eocystic oocysts which are confirmed by the results of regression and correlation analysis. The dependence of the Y oocyte species on seven characteristics has been proved. Conclusions. The morphological features that are mathematical expressions of morphometric dependences of the structure and identification of the species of turkey oocysts are determined with high accuracy. The relative error in determining the type of turkey eimerias does not exceed 2 %
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利用形态计量指标的回归分析鉴定火鸡emia的种类
的目标。用形态学参数鉴定火鸡卵囊的种类。材料和方法。从哈尔科夫地区家禽养殖场的火鸡自发性艾美耳虫病疑似病例和患者粪便中获得艾美耳虫卵囊作为研究材料。方法:寄生虫学、泌尿学、光镜学、形态学、数理统计学、相关与回归分析、方差分析等。结果。对255份火鸡艾美耳球虫卵囊进行形态计量学研究,根据鉴定指标(卵囊长度X1 μm;X2 -卵囊宽度,单位为μm;X3 -卵囊面积,单位μm;X4—模型椭圆的偏心率;X5 -卵囊宽度与长度之比;X6为模型椭圆极点在长、小轴上的最大曲率,X7为最小曲率,分别为μm, X8为存在- 1或不存在- 0极性颗粒),这是囊性卵囊结构形态依赖性的数学表达式,回归分析和相关分析结果证实了这一点。已经证实了Y卵母细胞种类对7个特征的依赖性。结论。形态学特征是火鸡卵囊结构和物种鉴定的形态学依赖的数学表达,具有很高的准确性。测定火鸡艾美耳属的相对误差不超过2%
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审稿时长
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