ارزیابی بافت شناسی انواع سلول های ماستوسیت در فلاپ پوستی به کمک سلول های بنیادی مزانشیمی مغز استخوان از طریق فاکتور رشد بیو لوژیکی

Abstract

J Babol Univ Med Sci; 20(3); Mar 2018; PP: 21-8 Received: Sep 26 2017, Revised: Dec 9 2017, Accepted: Jan 9 2018. ABSTRACT BACKGROUND AND OBJECTIVE: Skin flap is one of the most commonly used methods in plastic surgery. Postoperative skin flap necrosis is one of the complications of flap skin. The aim of this study was to evaluate the effect of bone marrow mesenchymal stem cells (BM-MSCs) and chick embryo extract (CEE) on mast cells in a randomized skin flap in rats. METHODS: In this experimental study, 40 male albino Wistar rats weighing 250 – 300 g were divided into four groups of 10 (control, CEE/BM-MSCs, CEE and BM-MSCs). Skin flap (30 × 80 mm) was created behind the animals. Surgery was performed on day zero and therapeutic intervention was done on the same day. Mesenchymal stem cells were extracted from rat bone marrow and were injected. CEE was prepared from a 9-day-old embryo of Marandi chicken. On the seventh day after the surgery, samples were assessed in term of type, and the total number of mast cells (type 1 to 3) in the transfer line. FINDINGS: The difference between the mean number of mast cells type 1 (3.67±1.91) (p=0.99), and type 3 (1.9±1.47) (p=0.384) was not significant in the study groups, but was statistically significant in type 2 (2.27±1.42) in different study groups (p=0.005). There was also a statistically significant difference between the mean total number of mast cells (2.32±0.84) in the BM-MSCs group and other experimental groups (p=0.001). CONCLUSION: Based on the results of this study, increase in mast cell type 2, the improvement of small vessels and decrease in mast cell type 3 lead to the reduction of scarring and fibrosis.