SYNTHESIS AND CHARACTERISTICS OF SULFATED CHITOSAN BASED ON CHITIN/CHITOSAN FROM ARTEMIA PARTHENOGENETICA CYSTS

Abstract

This article presents for the first-time the sulfated chitosan (SCS), synthesized on the basis of chitin/chitosan obtained from cysts of the Karakalpak population of Artemia parthenogenetica of the Aral Sea, and some of its physicochemical characteristics. Structural studies of chitin/chitosan and SCS were carried out by infrared spectroscopy. The IR spectrum data showed amide I (C=O-NHR) bands characteristic of chitin at 1640 cm-1 , and for chitosan absorption bands of the amino group (-NH2) at 1588 cm -1 . Signals at 870 and 614 cm-1 and 1125-1388 cm-1 in SCS comply with C-O-S and S=O bonds, which indicates the inclusion of sulfate groups in chitosan. The degree of substitution (DS) established by the gravimetric method, is in line with 1.32. The molecular weights of chitosan and SCS were calculated using size exclusion chromatography (SEC). According to the calculation of SEC for chitosan, an average molecular weight (MW) complies with 16 kDa, and for SCS - 1-3 kDa.