The Influence of Aia Tempayang on Interleukin-2 (IL2) Levels on Female Rattus Norvegicus Sprague Dawley Strains in Breast Cancer Prevention

Abstract

Background: Breast cancer is a condition in which the cells in the breast tissue grow rapidly and uncontrollably. Risk factors for cancer are those caused by carcinogenic factors, behavior, and diet. Breast cancer causes a decrease in immunity. It can be prevented by consuming high-antioxidant foods such as Aia Tempayang. Aia Tempayang is made from caesalpinia sappan l., scaphium scaphigerum/seeds and ocimum basilicum seeds, each of which contains an antioxidant compound that prevents cancer.Objectives: To analyze the effect of Aia Tempayang  on the decline of interleukin-2 (il-2) as a deterrent to breast cancer.Materials and Methods: Female Sprague Dawley was 35 days old n= 30, and divided into five groups: normal control group (K1); control groups induced by DMBA without intervention (K2); treatment group induced by DMBA + caesalpinia sappan l. 0.072 g + scaphium scaphigerum 0.012 g + basilicum seeds 0.045 g (X1); treatment group induced by DMBA + caesalpinia sappan l 0.144 g + scaphium scaphigerum 0.024 g + basilicum seeds 0.09 g (X2); and treatment group induced by DMBA + caesalpinia sappan l 0.288 g + scaphium scaphigerum 0.048 g + basilicum seeds 0.18 g (X3). After 35 days of intervention, serum IL-2 was analyzed using ELISA method. Data analysis used Paired-T Test, One Way ANOVA, and Post-Hoc Bonferroni follow-up test. Results: There was a significant difference in serum IL-2 (p=0,000) between supplementation groups found after intervention.The X1, X2, and X3 groups showed decreased of IL-2 to the K2 group without intervention.Conclusion: Aia tempayang was effective in reducing interleukin-2 levels in the group of mice induced by DMBA for 35 days with doses of  X1, X2 and X3. The dose closest to the normal group is the intervention group X3 with a dose of Caesalpinia sappan l 0.288 g + Scaphium scaphigerum 0.048 g + Basilicum seed 0.18 g.