Protective effects of Nymphaea lotus Linn (Nymphaeaceae) on L-NAME-induced tissular oxidative damages and erectile dysfunction in hypertensive male rat
K. Mireille, D. Désiré, Bilanda Danielle Claude, M. N. Y. Sandrine, Mballa Marguerite Francine, Ngoungoure Madeleine Chantal, O. Carolle, D. Théophile, Kamtchouing Pierre
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引用次数: 5
Abstract
To investigate Nymphaea lotus aqueous extract on L-NAME-mediated sexual dysfunction and tissular oxidative stress in male Wistar rats. Methods: Fifty adult male Wistar rats were randomly classified into 5 groups; Control, L-NAME (10 mg/kg), L-NAME + losartan (10 mg/kg), L-NAME + N. lotus at the dose of 75 mg/kg and 200 mg/kg. L-NAME was administered during 8 weeks, but others treatments started from the 4th week and were administered continuously with L-NAME (10 mg/kg) during 4 additional weeks. Results: L-NAME administration caused marked hit of sexual behaviour, specifically failure of penile insertion or difficulty to ejaculate during the test interval. Further L-NAME causes a significant decrease in antioxidant products as reduced gluthatione (GSH) and nitrites (NO) in aorta and penile tissues, as compared to control group. N. lotus co-treatment for 4 weeks increased markedly the erectile index and the ejaculation rates contrarily to losartan in comparison to negative control receiving only L-NAME. N. lotus, but not the positive drug losartan, induced a significant increase in the anti-oxidant enzymes activities and GSH and NO levels, as well as a significant decrease in MDA levels compared to L-NAME group. These results suggests N. lotus may provide significant protection against L-NAME-induced tissular oxidative damages by up-regulation of antioxidant systems and promotion of the vasodilator factors in chronic NO-deficient rats. These alleviating effects of Nymphaea lotus denote a pro-sexual, antioxidant and vasodilatatory properties that was more appreciated after histological examination where remodeling of aorta were visibly reduced. Conclusion: N. lotus may be considered as a potentially useful strategy to limit erectile dysfunction and toxicity associated with hypertension.