DETERMINING THE TANNIN CONTENT IN PISANG AMBON (Musa × paradisiaca L.) WITH THE POTENTIAL AS ANTHELMINTIC

Dwi Haryatmi, I. Susilowati
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Abstract

Tannins result from plants' secondary metabolism, closely associated with plant defense mechanisms against insects. Condensed tannins can disrupt the life cycle of parasitic nematodes starting from eggs, adult worms, and larvae. Currently, the antiparasitic properties of condensed tannins are being investigated as an alternative for controlling parasites. The people use the young Pisang Ambon (Musa × parasidiaca L.) as an anthelmintic. People generally use this part of the banana peel. Based on this, a study was conducted to determine the tannin content in the peel and fruit of Pisang Ambon (Musa × parasidiaca L.). The research was conducted by extracting the fruit and peel of young Pisang Ambon by maceration method using 96% ethanol as solvent. The Harborne method and Thin Layer Chromatography tested the presence of secondary metabolites. The tannin content was determined by the visible, ultraviolet spectrophotometric method. The qualitative results showed that the peel and fruit of Pisang Ambon contained condensed tannins. Meanwhile, the quantitative results showed that the tannin content of the fruit (54.98% w/w) was higher than the peel (14.32 %w/w).
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PISANG AMBON (Musa x paradisiaca L.)单宁含量的测定有驱虫的潜力
单宁来源于植物的次生代谢,与植物对昆虫的防御机制密切相关。浓缩单宁可以破坏寄生线虫从卵、成虫和幼虫开始的生命周期。目前,人们正在研究缩合单宁的抗寄生性能,以作为控制寄生虫的替代方法。人们使用年轻的Pisang Ambon (Musa x parasidiaca L.)作为驱虫剂。人们通常使用香蕉皮的这一部分。在此基础上,对枇杷果皮和果实中的单宁含量进行了测定。以96%乙醇为溶剂,采用浸渍法提取枇杷幼果和果皮。Harborne法和薄层色谱法检测了次生代谢物的存在。采用可见、紫外分光光度法测定单宁含量。定性分析结果表明,枇杷果皮和果实中含有浓缩单宁。定量结果表明,果实单宁含量(54.98% w/w)高于果皮(14.32% w/w)。
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