Comparative Study of the Bromelain Extracted from Pineapple Peel (Ananas Comosus) and Curaua Leaves (Ananas Erectifolius) Varieties White and Purple

T. Silva, I. P. Bresolin, I. Garrard, E. Tambourgi
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Abstract

Curaua is a fibrous plant found in northern of Brazil, is a Bromeliaceae that contains significant levels of bromelain, a group of proteolytic enzymes of high commercial value and wide application in pharmaceutical, food and cosmetics. The aim of this study was to extract bromelain from pineapple and curaua (white and purple) to evaluate enzyme activity and determining the optimum values of pH and temperature, in order to optimize the conditions of use and application for further purification. In the results, bromelain from white curaua has a pH optimum of 6.0 and optimum temperature of 40°C and 60°C. For the species purple the optimum pH is 6.0 to 7.0 and optimum temperature is 40°C. On the other hand, bromelain from pineapple peel has a pH optimum of 6.0 and 7.0 and temperature optimum at 40°C
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白、紫凤梨叶与凤梨皮中菠萝蛋白酶的比较研究
Curaua是一种产于巴西北部的纤维植物,是凤梨科的一种,含有大量的菠萝蛋白酶,这是一组具有很高商业价值的蛋白水解酶,在制药、食品和化妆品中有着广泛的应用。本研究的目的是从菠萝和curaua(白色和紫色)中提取菠萝蛋白酶,评价酶活性,确定最佳pH值和温度,以优化使用条件和进一步纯化应用。结果表明,产白瓜菠萝蛋白酶的最适pH为6.0,最适温度为40℃和60℃。紫色的适宜pH为6.0 ~ 7.0,适宜温度为40℃。另一方面,菠萝皮中菠萝蛋白酶的最适pH值为6.0和7.0,最适温度为40℃
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