{"title":"Genetic Relationship amongSome Wheat Genotypes Using Ten ISSR Markers","authors":"Melathkadem FarhoodAL Ghufaili, A. Al-Tamimi","doi":"10.9790/3008-1203063036","DOIUrl":null,"url":null,"abstract":"Ten of ISSRs (interSimple Sequence Repeats) markers were used for revealing genetic relationship, genetic diversity and DNA fingerprint of ten bread wheat (Triticumaestivum L.) genotypes. Primers varied among them in giving unique DNA fingerprint hence primers HB12, UBC811andUBC852 gave unique fingerprint for all ten studied wheat genotypes, others varied between 2-6 fingerprint genotypes while primer 17899A gave no unique fingerprint. High genetic distance was 0.661 while low genetic distance was 0.131.Cluster analysis (Phylogenetic tree) grouped studied genotypes in to two main groups ,the first small one included only one genotype while the other large main group included the rest genotypes which further divided in to two sub groups .ISSRs represent good marker for revealing genetic diversity and genotypes fingerprinting. The results could help plant breeder in breeding programs.","PeriodicalId":14548,"journal":{"name":"IOSR Journal of Pharmacy and Biological Sciences","volume":"1 1","pages":"30-36"},"PeriodicalIF":0.0000,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"IOSR Journal of Pharmacy and Biological Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.9790/3008-1203063036","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
Ten of ISSRs (interSimple Sequence Repeats) markers were used for revealing genetic relationship, genetic diversity and DNA fingerprint of ten bread wheat (Triticumaestivum L.) genotypes. Primers varied among them in giving unique DNA fingerprint hence primers HB12, UBC811andUBC852 gave unique fingerprint for all ten studied wheat genotypes, others varied between 2-6 fingerprint genotypes while primer 17899A gave no unique fingerprint. High genetic distance was 0.661 while low genetic distance was 0.131.Cluster analysis (Phylogenetic tree) grouped studied genotypes in to two main groups ,the first small one included only one genotype while the other large main group included the rest genotypes which further divided in to two sub groups .ISSRs represent good marker for revealing genetic diversity and genotypes fingerprinting. The results could help plant breeder in breeding programs.
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