Growth hormone modulates Trypanosoma cruzi infection in vitro

IF 1.6 4区 医学 Q4 CELL BIOLOGY Growth Hormone & Igf Research Pub Date : 2022-06-01 DOI:10.1016/j.ghir.2022.101460
Patricia Mora-Criollo , Reetobrata Basu , Yanrong Qian , Jaime A. Costales , Jaime Guevara-Aguirre , Mario J. Grijalva , John J. Kopchick
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引用次数: 3

Abstract

Objective

Chagas disease (CD) is caused by the protozoan parasite, Trypanosoma cruzi. It affects 7 to 8 million people worldwide and leads to approximately 50,000 deaths per year. In vitro and in vivo studies had demonstrated that Trypanosoma cruziinfection causes an imbalance in the hypothalamic-pituitary-adrenal (HPA) axis that is accompanied by a progressive decrease in growth hormone (GH) and prolactin (PRL) production. In humans, inactivating mutations in the GH receptor gene cause Laron Syndrome (LS), an autosomal recessive disorder. Affected subjects are short, have increased adiposity, decreased insulin-like growth factor-I (IGFI), increased serum GH levels, are highly resistant to diabetes and cancer, and display slow cognitive decline. In addition, CD incidence in these individuals is diminished despite living in highly endemic areas. Consequently, we decided to investigate the in vitro effect of GH/IGF-I on T. cruzi infection.

Design

We first treated the parasite and/or host cells with different peptide hormones including GH, IGFI, and PRL. Then, we treated cells using different combinations of GH/IGF-I attempting to mimic the GH/IGF-I serum levels observed in LS subjects.

Results

We found that exogenous GH confers protection against T. cruzi infection. Moreover, this effect is mediated by GH and not IGFI. The combination of relatively high GH (50 ng/ml) and low IGF-I (20 ng/ml), mimicking the hormonal pattern seen in LS individuals, consistently decreased T. cruzi infection in vitro.

Conclusions

The combination of relatively high GH and low IGF-I serum levels in LS individuals may be an underlying condition providing partial protection against T. cruzi infection.

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生长激素对克氏锥虫体外感染的调节作用
目的查加斯病(CD)是由克氏锥虫原虫引起的疾病。它影响到全世界700万至800万人,每年导致约5万人死亡。体外和体内研究表明,克氏锥虫感染导致下丘脑-垂体-肾上腺(HPA)轴失衡,并伴有生长激素(GH)和催乳素(PRL)分泌的逐渐减少。在人类中,生长激素受体基因的失活突变导致Laron综合征(LS),这是一种常染色体隐性遗传病。受影响的受试者身材矮小,肥胖增加,胰岛素样生长因子- 1 (IGFI)降低,血清生长激素水平升高,对糖尿病和癌症具有高度抵抗力,并表现出缓慢的认知能力下降。此外,尽管生活在高流行地区,这些人的乳糜泻发病率却有所下降。因此,我们决定研究GH/ igf - 1在体外对克氏锥虫感染的影响。我们首先用不同的肽激素(包括GH、IGFI和PRL)处理寄生虫和/或宿主细胞。然后,我们使用不同的GH/IGF-I组合处理细胞,试图模拟在LS受试者中观察到的GH/IGF-I血清水平。结果外源性生长激素对克氏锥虫感染具有保护作用。此外,这种作用是由生长激素介导的,而不是IGFI。相对较高的生长激素(50 ng/ml)和较低的IGF-I (20 ng/ml)的组合,模拟了LS个体的激素模式,在体外持续降低克氏体感染。结论LS患者血清中相对较高的生长激素和较低的igf - 1水平可能是对克氏t细胞感染提供部分保护的潜在条件。
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来源期刊
Growth Hormone & Igf Research
Growth Hormone & Igf Research 医学-内分泌学与代谢
CiteScore
3.30
自引率
0.00%
发文量
38
审稿时长
57 days
期刊介绍: Growth Hormone & IGF Research is a forum for research on the regulation of growth and metabolism in humans, animals, tissues and cells. It publishes articles on all aspects of growth-promoting and growth-inhibiting hormones and factors, with particular emphasis on insulin-like growth factors (IGFs) and growth hormone. This reflects the increasing importance of growth hormone and IGFs in clinical medicine and in the treatment of diseases.
期刊最新文献
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