Liquid Chromatography of Aflatoxins

Abstract

There are numerous reports on studies of aflatoxins, but there are only a few reports on the isolation and mutual separation of aflatoxins by liquid chromatography. Following the previous reports on the liquid chromatography of four kinds (B1, B2, G1 and G2) of aflatoxins, the authors carried out the chromatography of six kinds of aflatoxins including aflatoxins B2a and G2a. Various kinds of adsorbents and eluting solvents were examined, and then the good mutual separation of aflatoxins was obtained by using the Sephadex G-10 (CM), a newly prepared adsorbent containing carboxymethyl group, and pure water for eluting solvent. Aflatoxins G2a, B2a, G2, B2, G1 and B1 were eluted in this order.