La selección espermática mediante técnica de separación magnética de células activadas (MACS) en las técnicas de reproducción asistida

Abstract

The sperm integrity is essential to ensure reproductive competence. Several studies have shown that ejaculated sperm exhibit changes associated with apoptosis, in particular externalisation of phosphatidylserine, decreased MMP integrity, and DNA fragmentation.

The classification of magnetically activated cells with Annexin V microspheres recognises phosphatidylserine residues externalised on the surface of apoptotic spermatozoa. In addition, the use of Annexin V microspheres to select non-apoptotic spermatozoa reduces the percentage of altered cells with DNA fragmentation and aneuploidy, improving the possibility of pregnancy after assisted reproduction techniques.

In this review, our objective is to evaluate the possible value of activated cell magnetic classification technology in infertile patients undergoing assisted reproduction techniques.