Metabolit-induzierte inaktivierung von glutaminsynthetase aus Escherichia coli im zellfreien system

Abstract

A system is described in which glutaminine synthetase (EC 6.3.1.2) from Escherichia coli B is irreversibly inactivated by incubation with ATP, Mg²⁺ and gluatmine or glutamate in vitro. Under the conditions chose, the glutamyl transferase activity does not change significantly.

Mechanisms are discussed for the in activation of glutamine synthetase in vitro as well as the relationship of this process to the inactivation of the enzyme by NH₄⁺ in intact cells.