In silico approach for mining of potential drug targets from hypothetical proteins of bacterial proteome

Abstract

Protein consists of four levels structure which is primary, secondary, tertiary and quaternary structure. The structures are built based on the linking amino acids. For sequences of amino acids of lesser than fifty amino acids linked together are known as peptides and are usually the primary structure of the protein.1 Hence, the primary structure is described as linear chain blocks of amino acids. If the amino acids are more than fifty, it is described as polypeptides. Secondary structures of the protein are known as the “folding protein” where the polypeptides are either folded to α-helices, β-strands and random coil.2 The structures are folded by linking the C=O and N-H by hydrogen bonds that make the structures more stable.3 Tertiary structures are known as the whole structure of the protein in a 3-Dimensional shape (3D) of which the protein structures are folded. Quaternary structures are referred to as the spatial relationship or the interaction between the subunits of the protein or also known as the individuals’ polypeptide chain.