The Study on Activity of Bee Venom Phospholipase A2 from Apis mellifera caucasia L in Honey Bees

Abstract

This study investigates of phospholipase A2 activity in venom extracts from honey bees (Apis mellifera caucasica l) from different regions of Azerbaijan (on apiaries of surrounding area of Baku, Sumgait, Sheki, Balaken, Zagatala, Shamakhi). Collection of venom carried out in the spring, summer, autumn. The PLA2s activity in the venom was determined the titration method. The lowest phospholipase A2 activity was found in venom collected in suburb of Baku (134, 7 IU/mg), and highest in Zaqatala (183, 7 IU/mg). Phospholipase A2 activity was obtained in venom with respect to season of the year. The highest mean activity of phospholipase A2 in venom was found in summer season, the lowest activity is during autumn in all regions. The results of experimental data can be used for determining the biological activity of identification and standardization of honey bee venom.