Accumulation of cadmium (Cd) in T1 transgenic tobacco seedlings expressing metallothionein gene from Eleusine indica

N. Sidik, R. M. Yazid, Dhalila Zafirah Mohd Dahlan, B. Othman, I. Ismail
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引用次数: 4

Abstract

Cadmium (Cd) contamination of urban and agricultural soils is toxic to humans, animals and may cause negative effects on plant growth and crop production. The existing conventional methods are found to be not efficient to remove Cd from contaminated soil. The present experiment reports the analysis of nine T1 lines of transgenic tobacco carrying metallothionein gene (eiMT1) from Eleusine indica, with potential for high efficiency to remediate Cd in contaminated soils. Thirty-days old tobacco plants were treated with different concentrations of CdNO3 (0, 50, 100 and, 150 µmol) for seven days and the accumulation of Cd in the whole seedling was quantitatively determined by using atomic absorption spectrometer (AAS). All transgenic tobacco lines showed greater tolerance and accumulated higher level of Cd than the wild type with lines 18D, 20D1 and, 18C were among the highest (678.7, 623.0 and 611.9 mgkg-1 Cd, respectively). Meanwhile, transgenic tobacco lines 18B1 and 20D1 showed higher expression of eiMT1 gene. These results suggest that the cadmium accumulation in transgenic tobacco did not strictly associate with the expression level of eiMT1 gene. However, expression of eiMT1 greatly required for higher accumulation of Cd in transgenic tobacco seedling.
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表达烟草金属硫蛋白基因T1转基因烟草幼苗镉(Cd)的积累
城市和农业土壤的镉污染对人类和动物有毒,并可能对植物生长和作物生产造成负面影响。现有的常规方法对污染土壤中镉的去除效果不佳。本试验报道了9个携带烟中金属硫蛋白基因(eiMT1)的转基因烟草T1系,该烟草具有高效修复土壤镉污染的潜力。采用不同浓度的CdNO3(0、50、100和150µmol)处理30 d烟叶7 d,利用原子吸收光谱仪(AAS)定量测定全苗Cd积累量。所有转基因烟草株系对Cd的耐受性和累积量均高于野生型,其中18D、20D1和18C株系最高(分别为678.7、623.0和611.9 mgkg-1 Cd)。同时,转基因烟草株系18B1和20D1的eiMT1基因表达量较高。这些结果表明,转基因烟草中镉的积累与eiMT1基因的表达水平没有严格的关系。然而,eiMT1的表达对转基因烟草幼苗的Cd积累有很大的要求。
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