Surgical and clinical aspects of colorectal carcinoma and their correlation with BRAFV600E expression in Iraqi patients

Abstract

background:Colorectal cancer (CRC) the most common gastrointestinal cancer in the world . In Iraq it's fifth of the 5 commonest cancer between male 6.33% and fourth of commonest cancer in between female 4.35% .Co-morbidity ,mortality and the prognosis depended on several factors like age, gender ,stage , grade ,general health of patient , although the stage and grade are the most important factors . That is why different types and modality of diagnostic tools and methods had been liberated to the field of such a serious disease to altering it's progression. The oncogenic mutation in the kinase region of BRAF gene result in abnormal cellular growth , apoptosis ,resistance , tumor progression and metastasis.Objects /To high light on surgical and clinical criteria of colonic tumorsand specially focus on CRC in Iraqi patients .Alsoto analyses the efficacy of use immunohistochemical expression of BRAFV600E biomarker in colorectal tissues of Iraqi patients.Material and Methods/ In this study , a total of 90patients of colonic tumor referring to Al-Yarmouk Teaching Hospital ,all investigations done for them clinical ,laboratory , radiology and endoscopic with biopsy were done to them. Then after all cases with CRC (60 patients) under gone surgery each case according to standard surgical technique he needed and specimen send to histopathological examination , form each case 2 slides was done at a private lab. One For H&E stain and , second one for IHC.Results/In our study the CRC is more common in old age , males , left colon and most cases of high grade (III) and high stage (III and IV) and more than 5cm. The current study shown a correlation of BRAF Journal of University of Shanghai for Science and Technology ISSN: 1007-6735 Volume 23, Issue 6, June 2021 Page -345 expression with age, tumor size , tumor grade and stage of CRC patients is significant value , but with other variables are not significant correlation in CRC pateints.conclucion /According to our result most of the CRC cases occurs in the 7th decade of life and most of the cases were stage 3 tumor . We found significant correlation between BRAF expression and patient's age, tumor site , tumor size , pathological stage and histological grade. Key words/Colorectal cancer (CRC), BRAF and immunohistochemistry ( IHC)